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GSPT1 (NM_001130006) Human Untagged Clone
CAT＃: SC323160
GSPT1 (untagged)-Human G1 to S phase transition 1 (GSPT1), transcript variant 2
CNY 10,260.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | 551G9.2; eRF3a; ETF3A; GST1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC323160 representing NM_001130006.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGATCCGGGCAGTGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGGAGCAGCAGCGGCAGCAGCAGC AGCGACTCGGCGCCTGACTGCTGGGACCAGGCGGACATGGAAGCCCCCGGGCCGGGCCCTTGCGGCGGC GGCGGCTCCCTGGCGGCGGCGGCCGAGGCCCAGCGGGAGAACCTCAGCGCGGCCTTCAGCCGGCAACTC AACGTCAACGCCAAGCCCTTCGTGCCCAACGTCCACGCCGCCGAGTTCGTGCCGTCCTTCCTGCGGGGC CCGGCAGCGCCGCCACCCCCAGCTGGCGGCGCCGCCAATAACCACGGAGCCGGCAGCGGCGCGGGAGGC CGTGCGGCACCTGTGGAATCCTCTCAAGAGGAACAGTCATTGTGTGAAGGTTCAAATTCAGCTGTTAGC ATGGAACTTTCAGAACCTATTGAAAATGGAGAGACAGAAATGTCTCCAGAAGAATCATGGGAGCACAAA GAAGAAATAAGTGAAGCAGAGCCAGGGGGTGGTTCCTTGGGAGATGGAAGGCCGCCAGAGGAAAGTGCC CATGAAATGATGGAGGAGGAAGAGGAAATCCCAAAACCTAAGTCTGTGGTTGCACCGCCAGGTGCTCCT AAGAAAGAGCATGTAAATGTAGTATTCATTGGGCACGTAGATGCTGGCAAGTCAACCATTGGAGGACAA ATAATGTATTTGACTGGAATGGTTGACAAAAGGACGCTTGAAAAGTATGAAAGAGAAGCTAAAGAGAAA AACAGAGAAACTTGGTACTTGTCTTGGGCCTTAGACACAAATCAGGAAGAACGAGACAAGGGTAAAACA GTAGAAGTGGGTCGTGCCTATTTTGAAACCGAAAAGAAGCATTTCACAATTCTAGATGCCCCTGGCCAC AAGAGTTTTGTCCCAAATATGATTGGTGGTGCCTCTCAAGCTGATTTGGCTGTGCTGGTAATCTCAGCC AGGAAAGGAGAGTTTGAAACTGGATTTGAAAAAGGAGGACAGACAAGAGAACATGCAATGTTGGCAAAG ACAGCAGGTGTAAAACACCTAATTGTGCTAATTAATAAGATGGATGATCCAACAGTAAATTGGAGCAAT GAGAGATATGAAGAATGTAAGGAGAAACTAGTGCCATTTTTGAAAAAAGTTGGCTTCAATCCCAAAAAG GACATTCACTTTATGCCCTGCTCAGGACTTACTGGAGCAAATCTCAAAGAGCAGTCGGATTTCTGTCCT TGGTACATTGGATTACCGTTTATTCCATATCTGGATAATTTGCCGAACTTCAATAGATCAGTTGATGGA CCAATCAGGCTGCCAATTGTGGATAAGTACAAGGATATGGGCACTGTGGTCCTGGGAAAGCTGGAATCA GGATCTATTTGTAAAGGCCAGCAGCTTGTGATGATGCCAAACAAGCACAACGTGGAAGTTCTTGGAATA CTTTCCGATGATGTAGAGACTGATACCGTAGCCCCAGGTGAAAACCTCAAAATCAGACTGAAAGGAATT GAAGAAGAGGAGATTCTTCCAGGGTTTATACTTTGTGATCCTAATAATCTTTGTCATTCTGGACGCACA TTTGATGCCCAGATAGTGATTATAGAGCACAAATCCATCATCTGCCCAGGCTATAATGCGGTGCTGCAT ATTCATACCTGTATTGAGGAGGTGGAAATAACAGCCTTAATCTGCTTGGTAGACAAAAAATCAGGAGAA AAAAGTAAGACCCGACCCCGTTTTGTCAAACAAGATCAAGTATGCATTGCTCGCTTAAGGACAGCAGGA ACCATCTGCCTTGAGACCTTTAAAGACTTCCCTCAGATGGGTCGTTTCACCTTAAGAGATGAGGGTAAG ACCATTGCAATTGGAAAAGTTCTGAAACTGGTTCCAGAGAAAGACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001130006 |
| Insert Size | 1911 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001130006.1 |
| RefSeq Size | 7166 bp |
| RefSeq ORF | 1911 bp |
| Locus ID | 2935 |
| UniProt ID | P15170 |
| Protein Families | Transmembrane |
| MW | 68.6 kDa |
| Gene Summary | Involved in translation termination in response to the termination codons UAA, UAG and UGA (By similarity). Stimulates the activity of ETF1 (By similarity). Involved in regulation of mammalian cell growth (PubMed:2511002). Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons (PubMed:24486019).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site in an internal exon, compared to variant 1, resulting in an isoform (2) that is 1 aa shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226048 | GSPT1 (Myc-DDK-tagged)-Human G1 to S phase transition 1 (GSPT1), transcript variant 2 |
CNY 4,736.00 |
|
| RC226048L3 | Lenti ORF clone of Human G1 to S phase transition 1 (GSPT1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC226048L4 | Lenti ORF clone of Human G1 to S phase transition 1 (GSPT1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG226048 | GSPT1 (tGFP-tagged) - Human G1 to S phase transition 1 (GSPT1), transcript variant 2 |
CNY 4,370.00 |
