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Ataxin 3 (ATXN3) (NM_001127697) Human Untagged Clone
CAT＃: SC322954
ATXN3 (untagged)-Human ataxin 3 (ATXN3), transcript variant e
CNY 6,270.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AT3; ATX3; JOS; MJD; MJD1; SCA3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC322954 representing NM_001127697.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGTCCATCTTCCACGAGAAACAAGAAGGCTCACTTTGTGCTCAACATTGCCTGAATAACTTATTG CAAGGAGAATATTTTAGCCCTGTGGAATTATCCTCAATTGCACATCAGCTGGATGAGGAGGAGAGGATG AGAATGGCAGAAGGAGGAGTTACTAGTGAAGATTATCGCACGTTTTTACAGCAGCCTTCTGGAAATATG GATGACAGTGGTTTTTTCTCTATTCAGTGGTTTAACTTGAATTCTCTCTTGACGGGTCCAGAATTAATA TCAGATACATATCTTGCACTTTTCTTGGCTCAATTACAACAGGAAGGTTATTCTATATTTGTCGTTAAG GGTGATCTGCCAGATTGCGAAGCTGACCAACTCCTGCAGATGATTAGGGTCCAACAGATGCATCGACCA AAACTTATTGGAGAAGAATTAGCACAACTAAAAGAGCAAAGAGTCCATAAAACAGACCTGGAACGAGTG TTAGAAGCAAATGATGGCTCAGGAATGTTAGACGAAGATGAGGAGGATTTGCAGAGGGCTCTGGCACTA AGTCGCCAAGAAATTGACATGGAAGATGAGGAAGCAGATCTCCGCAGGGCTATTCAGCTAAGTATGCAA GGTAGTTCCAGAAACATATCTCAAGATATGACACAGACATCAGGTACAAATCTTACTTCAGAAGAGCTT CGGAAGAGACGAGAAGCCTACTTTGAAAAACAGCAGCAAAAGCAGCAACAGCAGCAGCAGCAGCAGCAG CAGGGGGACCTATCAGGACAGAGTTCACATCCATGTGAAAGGCCAGCCACCAGTTCAGGAGCACTTGGG AGTGATCTAGGTGATGCTATGAGTGAAGAAGACATGCTTCAGGCAGCTGTGACCATGTCTTTAGAAACT GTCAGAAATGATTTGAAAACAGAAGGAAAAAAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001127697 |
| Insert Size | 933 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001127697.2 |
| RefSeq Size | 6770 bp |
| RefSeq ORF | 933 bp |
| Locus ID | 4287 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 35.1 kDa |
| Gene Summary | Machado-Joseph disease, also known as spinocerebellar ataxia-3, is an autosomal dominant neurologic disorder. The protein encoded by this gene contains (CAG)n repeats in the coding region, and the expansion of these repeats from the normal 12-44 to 52-86 is one cause of Machado-Joseph disease. There is a negative correlation between the age of onset and CAG repeat numbers. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2016] Transcript Variant: This variant (e, also known as variant 4) is one of several transcript variants described in figure 2 of Bettencourt et al. (PMID: 19714377). This variant encodes isoform e (also known as isoform 4). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229152 | ATXN3 (Myc-DDK-tagged)-Human ataxin 3 (ATXN3), transcript variant e |
CNY 2,400.00 |
|
| RC229152L3 | Lenti ORF clone of Human ataxin 3 (ATXN3), transcript variant e, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC229152L4 | Lenti ORF clone of Human ataxin 3 (ATXN3), transcript variant e, mGFP tagged |
CNY 5,890.00 |
|
| RG229152 | ATXN3 (tGFP-tagged) - Human ataxin 3 (ATXN3), transcript variant e |
CNY 4,370.00 |
|
| SC327787 | ATXN3 (untagged)-Human ataxin 3 (ATXN3) transcript variant e |
CNY 6,270.00 |
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