VPS28 (NM_183057) Human Untagged Clone

CAT#: SC321875

VPS28 (untagged)-Human vacuolar protein sorting 28 homolog (S. cerevisiae) (VPS28), transcript variant 2



  "NM_183057" in other vectors (6)

CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
VPS28 mouse monoclonal antibody, clone OTI1A8 (formerly 1A8)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for NM_183057.1 CTTCCCGACCGCGAGCCGTCCAGGTCTCAGTGCTGTGCCCCCCCCAGAGCCTAGAGGATG
TTTCATGGGATCCCAGCCACGCCGGGCATAGGAGCCCCTGGGAACAAGCCGGAGCTGTAT
GAGGAAGTGAAGTTGTACAAGAACGCCCGGGAGAGGGAGAAGTACGACAACATGGCAGAG
CTGTTTGCGGTGGTGAAGACAATGCAAGCCCTGGAGAAGGCCTACATCAAGGACTGTGTC
TCCCCCAGCGAGTACACTGCAGCCTGCTCCCGGCTCCTGGTCCAATACAAAGCTGCCTTC
AGGCAGGTCCAGGGCTCAGAAATCAGCTCTATTGACGAATTCTGCCGCAAGTTCCGCCTG
GACTGCCCGCTGGCCATGGAGCGGATCAAGGAGGACCGGCCCATCACCATCAAGGACGAC
AAGGGCAACCTCAACCGCTGCATCGCAGACGTGGTCTCGCTCTTCATCACGGTCATGGAC
AAGCTGCGCCTGGAGATCCGCGCCATGGATGAGATCCAGCCCGACCTGCGAGAGCTGATG
GAGACCATGCACCGCATGAGCCACCTCCCACCCGACTTTGAGGGCCGCCAGACGGTCAGC
CAGTGGTGGGTGTCCCTCCCAGCCAGGCAGAGCCCCGCAGTGCCCGAGACCCTCCCAGCC
AGGCGGAGCCCCGCAGTGCCCCTGAGGCCCTCAGCCCCCACATGCCCTGTGCTGCACTCC
CAGGCTGCAGACCCTGAGCGGCATGTCGGCGTCAGATGAGCTGGACGACTCACAGGTGCG
TCAGATGCTGTTCGACCTGGAGTCAGCCTACAACGCCTTCAACCGCTTCCTGCATGCCTG
AGCCCGGGGCACTAGCCCTTGCACAGAAGGGCAGAGTCTGAGGCGATGGCTCCTGGTCCC
CTGTCCGCCACACAGGCCGTGGTCATCCACACAACTCACTGTCTGCAGCTGCCTGTCTGG
TGTCTGTCTTTGGTGTCAGAACTTTGGGGGCCGGGCCCCTCCCCACAATAAAGATGCTCT
CCGACCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_183057
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_183057.1, NP_898880.1
RefSeq Size 1160 bp
RefSeq ORF 702 bp
Locus ID 51160
UniProt ID Q9UK41
Protein Pathways Endocytosis
Gene Summary This gene encodes a protein subunit of the ESCRT-I complex (endosomal complexes required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. This complex can also be hijacked to facilitate the budding of enveloped viruses from the cell membrane. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]
Transcript Variant: This variant (2) contains an alternate segment in ther 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) is longer and has a distinct C-terminus, compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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