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Asparagine synthetase (ASNS) (NM_001673) Human Untagged Clone
CAT＃: SC321218
ASNS (untagged)-Human asparagine synthetase (glutamine-hydrolyzing) (ASNS), transcript variant 2
CNY 4,184.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ASNSD; TS11 |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_001673.2
GCCGCCCGTCAAGCTGTCCACATCCCTGGCCTCAGCCCGCCACATCACCCTGACCTGCTT
ACGCCCAGATTTTCTTCAATCACATCTGAATAAATCACTTGAAGAAAGCTTATAGCTTCA TTGCACCATGTGTGGCATTTGGGCGCTGTTTGGCAGTGATGATTGCCTTTCTGTTCAGTG TCTGAGTGCTATGAAGATTGCACACAGAGGTCCAGATGCATTCCGTTTTGAGAATGTCAA TGGATACACCAACTGCTGCTTTGGATTTCACCGGTTGGCGGTAGTTGACCCGCTGTTTGG AATGCAGCCAATTCGAGTGAAGAAATATCCGTATTTGTGGCTCTGTTACAATGGTGAAAT CTACAACCATAAGAAGATGCAACAGCATTTTGAATTTGAATACCAGACCAAAGTGGATGG TGAGATAATCCTTCATCTTTATGACAAAGGAGGAATTGAGCAAACAATTTGTATGTTGGA TGGTGTGTTTGCATTTGTTTTACTGGATACTGCCAATAAGAAAGTGTTCCTGGGTAGAGA TACATATGGAGTCAGACCTTTGTTTAAAGCAATGACAGAAGATGGATTTTTGGCTGTATG TTCAGAAGCTAAAGGTCTTGTTACATTGAAGCACTCCGCGACTCCCTTTTTAAAAGTGGA GCCTTTTCTTCCTGGACACTATGAAGTTTTGGATTTAAAGCCAAATGGCAAAGTTGCATC CGTGGAAATGGTTAAATATCATCACTGTCGGGATGTACCCCTGCACGCCCTCTATGACAA TGTGGAGAAACTCTTTCCAGGTTTTGAGATAGAAACTGTGAAGAACAACCTCAGGATCCT TTTTAATAATGCTGTAAAGAAACGTTTGATGACAGACAGAAGGATTGGCTGCCTTTTATC AGGGGGCTTGGACTCCAGCTTGGTTGCTGCCACTCTGTTGAAGCAGCTGAAAGAAGCCCA AGTACAGTATCCTCTCCAGACATTTGCAATTGGCATGGAAGACAGCCCCGATTTACTGGC TGCTAGAAAGGTGGCAGATCATATTGGAAGTGAACATTATGAAGTCCTTTTTAACTCTGA GGAAGGCATTCAGGCTCTGGATGAAGTCATATTTTCCTTGGAAACTTATGACATTACAAC AGTTCGTGCTTCAGTAGGTATGTATTTAATTTCCAAGTATATTCGGAAGAACACAGATAG CGTGGTGATCTTCTCTGGAGAAGGATCAGATGAACTTACGCAGGGTTACATATATTTTCA CAAGGCTCCTTCTCCTGAAAAAGCCGAGGAGGAGAGTGAGAGGCTTCTGAGGGAACTCTA TTTGTTTGATGTTCTCCGCGCAGATCGAACTACTGCTGCCCATGGTCTTGAACTGAGAGT CCCATTTCTAGATCATCGATTTTCTTCCTATTACTTGTCTCTGCCACCAGAAATGAGAAT TCCAAAGAATGGGATAGAAAAACATCTCCTGAGAGAGACGTTTGAGGATTCCAATCTGAT ACCCAAAGAGATTCTCTGGCGACCAAAAGAAGCCTTCAGTGATGGAATAACTTCAGTTAA GAATTCCTGGTTTAAGATTTTACAGGAATACGTTGAACATCAGGTTGATGATGCAATGAT GGCAAATGCAGCCCAGAAATTTCCCTTCAATACTCCTAAAACCAAAGAAGGATATTACTA CCGTCAAGTCTTTGAACGCCATTACCCAGGCCGGGCTGACTGGCTGAGCCATTACTGGAT GCCCAAGTGGATCAATGCCACTGACCCTTCTGCCCGCACGCTGACCCACTACAAGTCAGC TGTCAAAGCTTAGGTGGTCTTTATGCTGTAATGTGAAAGCAAATATTTCTTCGTGTTGGA TGGGGACTGTGGGTAGATAGGGGAACAATGAGAGTCAACTCAGGCTAACTTGGGTGTGAA AAAAATAAAAGTCCTAAATCTAAAAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_001673 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001673.2, NP_001664.2 |
| RefSeq Size | 1994 bp |
| RefSeq ORF | 1686 bp |
| Locus ID | 440 |
| UniProt ID | P08243 |
| Domains | GATase_2, Asn_synthase |
| Protein Families | Druggable Genome |
| Protein Pathways | Alanine, aspartate and glutamate metabolism, Metabolic pathways, Nitrogen metabolism |
| Gene Summary | The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3, and 7 encode the same isoform (a). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Asparagine couples mitochondrial respiration to ATF4 activity and tumor growth
,Krall, AS;Mullen, PJ;Surjono, F;Momcilovic, M;Schmid, EW;Halbrook, CJ;Thambundit, A;Mittelman, SD;Lyssiotis, CA;Shackelford, DB;Knott, SRV;Christofk, HR;,
Cell metabolism
,PubMed ID 33609439
[ASNS]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201297 | ASNS (Myc-DDK-tagged)-Human asparagine synthetase (glutamine-hydrolyzing) (ASNS), transcript variant 2 |
CNY 4,176.00 |
|
| RC201297L3 | Lenti ORF clone of Human asparagine synthetase (glutamine-hydrolyzing) (ASNS), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201297L4 | Lenti ORF clone of Human asparagine synthetase (glutamine-hydrolyzing) (ASNS), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG201297 | ASNS (tGFP-tagged) - Human asparagine synthetase (glutamine-hydrolyzing) (ASNS), transcript variant 2 |
CNY 5,776.00 |
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