TFIIF (GTF2F1) (NM_002096) Human Untagged Clone

CAT#: SC320316

GTF2F1 (untagged)-Human general transcription factor IIF, polypeptide 1, 74kDa (GTF2F1)



  "NM_002096" in other vectors (7)

CNY 3,856.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
GTF2F1 mouse monoclonal antibody, clone OTI4B10 (formerly 4B10)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms BTF4; RAP74; TF2F1; TFIIF
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for NM_002096.1 GTGCCAGAGGCGCCTAGGGTTGGGGTCCTCGCTCAGGCACAGAGACCCGACACCGAGCGG
CGGCTTCCCCGGGATCGAGGGACGCGCACGCCAGAGGAGACGAAAGGAACCCGGGTCGGA
CCAGATCGGAACCACTGACCATTGCCCATGGCGGCCCTAGGCCCTAGCAGCCAGAATGTC
ACTGAATACGTCGTTCGAGTTCCTAAGAATACAACCAAAAAATATAACATCATGGCTTTT
AATGCAGCCGACAAAGTCAACTTTGCTACGTGGAATCAGGCTCGGCTGGAGCGGGACTTG
AGCAACAAGAAAATCTACCAAGAGGAGGAGATGCCCGAATCGGGCGCGGGCAGTGAGTTC
AACCGCAAGCTTCGGGAGGAGGCTCGGAGGAAGAAGTACGGCATCGTCCTCAAGGAGTTC
CGGCCCGAGGACCAGCCCTGGCTGCTCCGGGTCAACGGCAAATCAGGCAGGAAGTTCAAG
GGCATCAAGAAGGGAGGCGTAACAGAGAACACGTCCTACTACATCTTCACCCAGTGCCCC
GACGGGGCCTTCGAGGCCTTCCCCGTGCACAACTGGTACAATTTCACACCGCTGGCCCGG
CATCGCACGCTCACTGCCGAGGAGGCCGAGGAGGAGTGGGAGAGGAGGAACAAGGTGCTG
AACCACTTCAGCATCATGCAGCAGCGGCGGCTCAAGGATCAGGACCAGGACGAGGATGAG
GAGGAGAAGGAGAAACGTGGCCGCAGGAAGGCGAGCGAGCTGCGCATCCACGACCTGGAG
GACGACCTGGAGATGTCGTCCGATGCCAGTGATGCCAGTGGTGAGGAGGGGGGCAGAGTC
CCCAAGGCCAAGAAGAAGGCGCCGCTGGCCAAGGGCGGCAGGAAAAAGAAGAAGAAGAAG
GGTTCAGACGACGAGGCCTTCGAGGACAGCGATGATGGGGACTTCGAGGGCCAAGAGGTA
GACTACATGTCAGACGGCTCCAGTAGCTCCCAAGAAGAGCCTGAGAGCAAGGCCAAGGCG
CCGCAGCAGGAGGAGGGGCCCAAGGGTGTCGATGAGCAGAGCGACAGTAGTGAGGAGAGT
GAGGAGGAGAAGCCGCCTGAGGAGGACAAGGAGGAGGAGGAGGAGAAGAAGGCACCCACC
CCGCAGGAGAAGAAGCGCAGGAAAGACAGCAGCGAGGAGTCGGACAGCTCAGAGGAGAGC
GACATTGACAGCGAGGCCTCCTCAGCCCTCTTCATGGCGAAGAAGAAGACGCCACCCAAG
AGAGAGCGGAAGCCGTCGGGAGGGAGCTCAAGGGGCAACAGCCGCCCAGGCACGCCCAGC
GCAGAGGGTGGCAGCACCTCCTCCACCCTGCGGGCGGCTGCCAGCAAACTCGAGCAAGGG
AAGCGGGTGAGCGAGATGCCTGCAGCCAAGCGGTTGCGGCTGGACACGGGACCCCAGAGC
CTGTCTGGGAAGTCGACACCCCAGCCACCATCAGGCAAGACAACACCCAACAGCGGCGAC
GTGCAGGTGACTGAGGATGCCGTGCGCCGCTACCTGACACGGAAGCCCATGACCACTAAG
GACCTGCTGAAAAAGTTCCAGACCAAGAAGACAGGGCTGAGCAGCGAGCAGACAGTGAAC
GTGTTGGCCCAGATCCTCAAGCGACTCAACCCCGAGCGCAAGATGATCAACGACAAAATG
CACTTCTCCCTCAAGGAGTGAGGCTTGGTCCAATACATGGCTCTGCCCCCCAGAACTTAA
GGCTCTCACTGCCCCTTCGCCATCCTAGAGTGAGGCTCTGTCCAATACATGGCTCTGCCC
TCCAGAACTTCAGGCTCTCAGTGACCCTTCGACATCCTGCTTGCTCCCTGACTCCCAGGG
CCCCGTAGTTAGCAATTCTGGAAAAGTTAAGCCATCTCCTCCTCTGGCCCTTCCTTCTGG
AATCTTCAGATGCCTGTTAGGCCTTCTTATTGTCCTCCTCCTCCTGGCTCGGCCTCCCTC
ACACTGACCAAGGGCCTGTGCTGCCCACTGGGTAACTTCTACAGTTCTCCCTTCCACTTC
CCTAAGTCTCTCTTCAGCTGTGACTTATCCACCACATAGCCCAGTAAGTCTTCAGTTTTG
GCTGGGCATGATGGTGAGCGCCTGCAGTCCCAGCTACTTGGGAGGCTAAGCCCAGTTCAA
GGCTGCAGTGAACTATGATGGTGCCACTGCATTCCAGCCTGGGTGACAGAATGAAATCCT
GGCACAAAAAAAAAAAAAAGTAGCCAGGCATGGTGGCGGGAGCCTGTTGTCCCAGCTGTT
CCGTAGGCTGAGGCACGAGATTCACTTGAACCTGGGAGGTGGAGGTTGCTGTGAGCTGAC
ACCATGCCACTGCACTCCAGCCTGGGTGACAGTGAGACTCTGTCTCAATAAATAAAAAAT
AATAATAAATTGAAAAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_002096
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_002096.1, NP_002087.1
RefSeq Size 2440 bp
RefSeq ORF 1554 bp
Locus ID 2962
UniProt ID P35269
Protein Families Druggable Genome, Transcription Factors
Protein Pathways Basal transcription factors
Gene Summary TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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