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Axin 1 (AXIN1) (NM_181050) Human Untagged Clone
CAT＃: SC319963
AXIN1 (untagged)-Human axin 1 (AXIN1), transcript variant 2
CNY 6,056.00
CNY 7,220.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AXIN; PPP1R49 |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_181050.1
GCTGCCGCTGAGCGCATGGGCCGGGCCGCGCCGCGCCGCTCCGGGAGCCGGGCCCGGGGT
CCCGCCACCACCGCGCGCGGGACAGATTGATTCACTTTGGAGCTGTAAGTACTGATGTAT TAGGGTGCAGCGCTCATTGTTCCTTGACGCAGAGTCCCAAAATGAATATCCAAGAGCAGG GTTTCCCCTTGGACCTCGGAGCAAGTTTCACCGAAGATGCTCCCCGACCCCCAGTGCCTG GTGAGGAGGGAGAACTGGTGTCCACAGACCCGAGGCCCGCCAGCTACAGTTTCTGCTCCG GGAAAGGTGTTGGCATTAAAGGTGAGACTTCGACGGCCACTCCGAGGCGCTCGGATCTGG ACCTGGGGTATGAGCCTGAGGGCAGTGCCTCCCCCACCCCACCATACTTGAAGTGGGCTG AGTCACTGCATTCCCTGCTGGATGACCAAGATGGGATAAGCCTGTTCAGGACTTTCCTGA AGCAGGAGGGCTGTGCCGACTTGCTGGACTTCTGGTTTGCCTGCACTGGCTTCAGGAAGC TGGAGCCCTGTGACTCGAACGAGGAGAAGAGGCTGAAGCTGGCGAGAGCCATCTACCGAA AGTACATTCTTGATAACAATGGCATCGTGTCCCGGCAGACCAAGCCAGCCACCAAGAGCT TCATAAAGGGCTGCATCATGAAGCAGCTGATCGATCCTGCCATGTTTGACCAGGCCCAGA CCGAAATCCAGGCCACTATGGAGGAAAACACCTATCCCTCCTTCCTTAAGTCTGATATTT ATTTGGAATATACGAGGACAGGCTCGGAGAGCCCCAAAGTCTGTAGTGACCAGAGCTCTG GGTCAGGGACAGGGAAGGGCATATCTGGATACCTGCCGACCTTAAATGAAGATGAGGAAT GGAAGTGTGACCAGGACATGGATGAGGACGATGGCAGAGACGCTGCTCCCCCCGGAAGAC TCCCTCAGAAGCTGCTCCTGGAGACAGCTGCCCCGAGGGTCTCCTCCAGTAGACGGTACA GCGAAGGCAGAGAGTTCAGGTATGGATCCTGGCGGGAGCCAGTCAACCCCTATTATGTCA ATGCCGGCTATGCCCTGGCCCCAGCCACCAGTGCCAACGACAGCGAGCAGCAGAGCCTGT CCAGCGATGCAGACACCCTGTCCCTCACGGACAGCAGCGTGGATGGGATCCCCCCATACA GGATCCGTAAGCAGCACCGCAGGGAGATGCAGGAGAGCGTGCAGGTCAATGGGCGGGTGC CCCTACCTCACATTCCCCGCACGTACCGGGTGCCGAAGGAGGTCCGCGTGGAGCCTCAGA AGTTCGCGGAGGAGCTCATCCACCGCCTGGAGGCTGTGCAGCGCACGCGGGAGGCCGAGG AGAAGCTGGAGGAGCGGCTGAAGCGCGTGCGCATGGAGGAGGAAGGTGAGGACGGCGATC CATCGTCAGGGCCCCCAGGGCCGTGTCACAAGCTGCCTCCCGCCCCCGCTTGGCACCACT TCCCGCCCCGCTGTGTGGACATGGGCTGTGCCGGGCTCCGGGATGCACACGAGGAGAACC CTGAGAGCATCCTGGACGAGCACGTACAGCGTGTGCTGAGGACACCTGGCCGCCAGTCGC CTGGGCCTGGCCATCGCTCCCCGGACAGTGGGCACGTGGCCAAGATGCCAGTGGCACTGG GGGGTGCCGCCTCGGGGCACGGGAAGCACGTACCCAAGTCAGGGGCGAAGCTGGACGCGG CCGGCCTGCACCACCACCGACACGTCCACCACCACGTCCACCACAGCACAGCCCGGCCCA AGGAGCAGGTGGAGGCCGAGGCCACCCGCAGGGCCCAGAGCAGCTTCGCCTGGGGCCTGG AACCACACAGCCATGGGGCAAGGTCCCGAGGCTACTCAGAGAGTGTTGGCGCTGCCCCCA ACGCCAGTGATGGCCTCGCCCACAGTGGGAAGGTGGGCGTGGCGTGCAAAAGAAATGCCA AGAAGGCTGAGTCGGGGAAGAGCGCCAGCACCGAGGTGCCAGGTGCCTCGGAGGATGCGG AGAAGAACCAGAAAATCATGCAGTGGATCATTGAGGGGGAAAAGGAGATCAGCAGGCACC GCAGGACCGGCCACGGGTCTTCGGGGACGAGGAAGCCACAGCCCCATGAGAACTCCAGAC CCTTGTCCCTTGAGCACCCCTGGGCCGGCCCTCAGCTCCGGACCTCCGTGCAGCCCTCCC ACCTCTTCATCCAAGACCCCACCATGCCACCCCACCCAGCTCCCAACCCCCTAACCCAGC TGGAGGAGGCGCGCCGACGTCTGGAGGAGGAAGAAAAGAGAGCCAGCCGAGCACCCTCCA AGCAGAGGACAAGATCGCAGAGGAAGGTGGGCGGCGGGAGTGCCCAGCCGTGTGACAGCA TCGTTGTGGCGTACTACTTCTGCGGGGAACCCATCCCCTACCGCACCCTGGTGAGGGGCC GCGCTGTCACCCTGGGCCAGTTCAAGGAGCTGCTGACCAAAAAGGGCAGCTACAGATACT ACTTCAAGAAAGTGAGCGACGAGTTTGACTGTGGGGTGGTGTTTGAGGAGGTTCGAGAGG ACGAGGCCGTCCTGCCCGTCTTTGAGGAGAAGATCATCGGCAAAGTGGAGAAGGTGGACT GATAGGCTGGTGGGCTGGCCGCTGTGCCAGGCGAGGCCCTTGGCGGGCACGGGTGTCACG GCCAGGCAGATGACCTCGTACTCAGGAGCCCGATGGGGAACAGTGTTGGGTGTACCACCC ATCCCTGTGGTCTACCCGTGTCTAGAGGCAGGTAGGGGGTCCCTCCAAGTGGTCCACAAG CTTCTGTCCTGCCCCCAAGGAGGCAGCCTGGACCACTCCTCATAGCAATACTTGGAGGGC CCAGCCCAAGTGAGGCAGCCGAGGTCCCTGCTGCCAGCTTCAGGTGACCCCCCCCCATCC CCCGGCACCTCCCTTGGGCACGTGTGCTGGGATCTACTTTCCCTCTGGGATTTGCCCACG TACCCAGGTCTGGCTGGGGCCCAGGCCCGGATGCAGAGGCCTGCAGGGCCTCTGTCAATT GTACGCGCCACCGAGTGCCTTCAACACAGCTTGTCTCTTGCCTGCCACTGTGTGAATCGG CGACGGAGCACTGCACCTGCCTCCAGCCGCCGGCTGTGCAGTCCTGGGTCCTCCTTTCTG AGGGCCCGTGTAAATATGTACATTTCTCAGGCTAGGCCAGCAGGGGCTGCCCGAGTCTGT TTTTCATGCGATGACACTTGTACAATTAATTATCTTTTCAAAGGTACTTGGATAATAATG AAATAAAACTGTTTTTGAACCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_181050 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_181050.1, NP_851393.1 |
| RefSeq Size | 3369 bp |
| RefSeq ORF | 2481 bp |
| Locus ID | 8312 |
| UniProt ID | O15169 |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - Wnt Signaling pathway |
| Protein Pathways | Basal cell carcinoma, Colorectal cancer, Endometrial cancer, Pathways in cancer, Wnt signaling pathway |
| Gene Summary | This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016] Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, compared to variant 1. The resulting protein (isoform b) is shorter, compared to isoform a. An upstream in-frame AUG is present that could extend the N-terminus of this isoform by 49 aa; however, there is no evidence that the upstream AUG is used, so the shorter N-terminus is being annotated. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Identification and Characterization of a Small-Molecule Inhibitor of Wnt Signaling in Glioblastoma Cells
,Alessandra De Robertis, Silvia Valensin, Marco Rossi, Patrizia Tunici, Margherita Verani, Antonella De Rosa, Cinzia Giordano, Maurizio Varrone, Arianna Nencini, Carmela Pratelli, Tiziana Benicchi, Annette Bakker, Jeffrey Hill, Kanda Sangthongpitag, Vishal Pendharkar, Boping Liu, Fui Mee Ng, Siew Wen Then, Shi Jing Tai, Seong-Moon Cheong, Xi He, Andrea Caricasole, and Massimiliano Salerno,
Mol. Cancer Ther., Jul 2013; 12: 1180 - 1189.
,PubMed ID 23619303
[Axin1]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208349 | AXIN1 (Myc-DDK-tagged)-Human axin 1 (AXIN1), transcript variant 2 |
CNY 6,048.00 |
|
| RC208349L1 | Lenti ORF clone of Human axin 1 (AXIN1), transcript variant 2, Myc-DDK-tagged |
CNY 8,448.00 |
|
| RC208349L2 | Lenti ORF clone of Human axin 1 (AXIN1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC208349L3 | Lenti ORF clone of Human axin 1 (AXIN1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208349L4 | Lenti ORF clone of Human axin 1 (AXIN1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG208349 | AXIN1 (tGFP-tagged) - Human axin 1 (AXIN1), transcript variant 2 |
CNY 7,648.00 |
