ATF1 (NM_005171) Human Untagged Clone

CAT#: SC319835

ATF1 (untagged)-Human activating transcription factor 1 (ATF1)



  "NM_005171" in other vectors (6)

CNY 2,400.00

CNY 2,950.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
ATF1 mouse monoclonal antibody, clone OTI2B4 (formerly 2B4)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms EWS-ATF1; FUS/ATF-1; TREB36
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_005171.2 GAGGCGGCAGCCACAGTTGATTATGGAAGATTCCCACAAGAGTACCACGTCAGAGACAGC
ACCTCAACCTGGTTCAGCAGTTCAGGGAGCTCACATTTCTCATATTGCTCAACAGGTATC
ATCTTTATCAGAAAGTGAGGAGTCCCAGGACTCATCCGACAGCATAGGCTCCTCACAGAA
AGCCCACGGGATCCTAGCACGGCGCCCATCTTACAGAAAAATTTTGAAAGACTTATCTTC
TGAAGATACACGGGGCAGAAAAGGAGACGGAGAAAATTCTGGAGTTTCTGCTGCTGTCAC
TTCTATGTCTGTTCCAACTCCCATCTATCAGACTAGCAGCGGACAGTATATTGCCATTGC
CCCAAATGGAGCCTTACAGTTGGCAAGTCCAGGCACAGATGGAGTACAGGGACTTCAGAC
ATTAACCATGACAAATTCAGGCAGTACTCAGCAAGGTACAACTATTCTTCAGTATGCACA
GACCTCTGATGGACAGCAGATACTTGTGCCCAGCAATCAGGTGGTCGTACAAACTGCATC
AGGAGATATGCAAACATATCAGATCCGAACTACACCTTCAGCTACTTCTCTGCCACAAAC
TGTGGTGATGACATCTCCTGTGACTCTCACCTCTCAGACAACTAAGACAGATGACCCCCA
ATTGAAAAGAGAAATAAGGTTAATGAAAAACAGAGAAGCTGCTCGAGAATGTCGCAGAAA
GAAGAAAGAATATGTGAAATGCCTGGAAAACCGAGTTGCAGTCCTGGAAAATCAAAATAA
AACTCTAATAGAAGAGTTAAAAACTTTGAAGGATCTTTATTCCAATAAAAGTGTTTGATT
CCTAAGAAAGAAAATATTTTTGTGGACATGCATAAAAATTAAATGGATTTCCTAGTGGAG
TTTTATAAATTAAAAGGTCAAAACTGAAGCTTTTTATTTAGGCTTTTCCAAATCAAGGAT
AAATATCTTACGCACGATATCTAGTGACAGAGGAGAAAGTGGAAAATGACCTCAAGGAAG
CTACGGGCACAACTGGAAGCCTTGTAGAAATTAAACATATTCAAGGAGCAAGAAATGAAC
TTTCAGCAGTCTAAATTTTCTAAATAACCAATAGTTGCCAATCTAAAGTGGCAGAGAAGA
TGAAATTTGATAAACTGAATTTTTTTTAAAAATCCATTTACCCTACAGGTTTGCATTTGT
TTGCTGAAATTTACCTTTTTTTAGTTATATATATGTGTGTGTGTGTGTGTAATTTCTGCC
AATAAATTCTAAATTACAAAGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_005171
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_005171.2, NP_005162.1
RefSeq Size 1568 bp
RefSeq ORF 816 bp
Locus ID 466
UniProt ID P18846
Domains pKID, BRLZ
Protein Families Druggable Genome, Transcription Factors
Gene Summary This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in angiomatoid fibrous histiocytoma and clear cell sarcoma. This gene has a pseudogene on chromosome 6. [provided by RefSeq, Aug 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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