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RRP8 (NM_015324) Human Untagged Clone
CAT＃: SC319409
RRP8 (untagged)-Human ribosomal RNA processing 8, methyltransferase, homolog (yeast) (RRP8)
CN¥ 3,656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | KIAA0409; NML |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_015324.2
CTGAGCGCTCCGACTTCCAGAGGAGCGCTGTGCACGTGGAGAAGAGCGGGGACTCGGCGA
CCCTGCCCTCCCGACCCTCATGTTCGAAGAGCCTGAGTGGGCCGAGGCGGCCCCAGTAGC CGCGGGCCTTGGGCCCGTAATCTCACGACCTCCGCCTGCGGCCTCCTCGCAAAACAAGGG CTCCAAGCGCCGCCAGCTCTTGGCCACATTACGGGCCCTAGAGGCAGCATCTCTTTCCCA GCATCCCCCCAGCCTATGTATAAGTGACTCTGAGGAGGAGGAGGAGGAAAGGAAGAAGAA ATGCCCCAAAAAGGCATCATTTGCCAGTGCCTCTGCTGAAGTAGGGAAGAAAGGGAAGAA GAAATGTCAAAAACAGGGCCCACCTTGCAGTGACTCTGAGGAAGAAGTAGAAAGGAAGAA GAAATGCCACAAACAGGCTCTTGTTGGCAGTGACTCTGCTGAAGATGAGAAAAGAAAGAG GAAATGCCAGAAACATGCCCCTATAAATTCAGCCCAGCACCTGGACAATGTTGACCAAAC AGGTCCCAAAGCCTGGAAGGGTAGTACTACAAATGATCCACCAAAGCAAAGCCCTGGGTC CACTTCCCCTAAACCCCCTCATACATTAAGCCGCAAGCAGTGGCGGAACCGGCAAAAGAA TAAGAGAAGATGTAAGAACAAGTTTCAGCCACCTCAGGTGCCAGACCAGGCCCCAGCTGA GGCCCCCACAGAGAAGACAGAGGTGTCTCCTGTTCCCAGGACAGACAGCCATGAGGCTCG GGCAGGGGCTTTGCGAGCCCGCATGGCACAGCGGCTGGATGGGGCCCGATTTCGCTACCT CAATGAACAGTTGTACTCAGGGCCCAGCAGTGCTGCACAGCGTCTCTTCCAGGAAGACCC TGAGGCTTTTCTTCTCTACCACCGCGGCTTCCAGAGCCAAGTGAAGAAGTGGCCACTGCA GCCAGTGGACCGCATCGCCAGGGATCTTCGCCAGCGGCCTGCATCCCTAGTGGTGGCTGA CTTCGGCTGTGGGGATTGCCGCTTGGCTTCAAGTATCCGGAACCCTGTGCATTGCTTTGA CTTGGCTTCTCTGGACCCTAGGGTCACTGTGTGTGACATGGCCCAGGTTCCTCTGGAGGA TGAGTCTGTGGATGTGGCTGTGTTTTGCCTTTCACTGATGGGAACCAACATCAGGGACTT CCTAGAGGAGGCAAATAGAGTACTGAAGCCAGGGGGTCTCCTGAAAGTGGCTGAGGTCAG CAGCCGCTTTGAGGATGTTCGAACCTTTCTGCGGGCTGTGACCAAGCTAGGCTTCAAGAT TGTCTCCAAGGACCTGACCAACAGCCATTTCTTCTTGTTTGATTTCCAAAAGACTGGGCC CCCTCTGGTAGGGCCCAAGGCTCAGCTTTCAGGCCTGCAGCTTCAGCCATGTCTCTACAA GCGCAGGTGACCTCTGGATCTTCCTTGAAAGGGGAGGCAGATCTCAAACTCCAGGCTCAG AACTGTGAAGACTGTTTCCAGCCTGGCTGTGAGCCAAGACCTGGTTCCTGGTGGACCCTG AGGACAAAGTGTGATAAAACCTCTGGCTCAGACTTGCTCTACTGAAGGCTTCTTGGTTAT AAGATGCATAAAGTCACTGGGGCTAGCTAAACAATAAAGAGTTTATTGTGAGAAAAAAAA AAAAAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_015324 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015324.2, NP_056139.1 |
| RefSeq Size | 1699 bp |
| RefSeq ORF | 1371 bp |
| Locus ID | 23378 |
| UniProt ID | O43159 |
| Domains | DUF691 |
| Gene Summary | Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200844 | RRP8 (Myc-DDK-tagged)-Human ribosomal RNA processing 8, methyltransferase, homolog (yeast) (RRP8) |
CN¥ 3,656.00 |
|
| RC200844L3 | Lenti ORF clone of Human ribosomal RNA processing 8, methyltransferase, homolog (yeast) (RRP8), Myc-DDK-tagged |
CN¥ 5,890.00 |
|
| RC200844L4 | Lenti ORF clone of Human ribosomal RNA processing 8, methyltransferase, homolog (yeast) (RRP8), mGFP tagged |
CN¥ 5,890.00 |
|
| RG200844 | RRP8 (tGFP-tagged) - Human ribosomal RNA processing 8, methyltransferase, homolog (yeast) (RRP8) |
CN¥ 5,256.00 |
