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CDC27 (NM_001114091) Human Untagged Clone
CAT＃: SC318990
CDC27 (untagged)-Human cell division cycle 27 homolog (S. cerevisiae) (CDC27), transcript variant 1
CNY 6,088.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E; H-NUC; HNUC; NUC2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_001114091 edited
CCCCGGTAACGGTCGCTGGTGAGTTTAAATGAGCAGGGGCTGGCCGGGCCGGAGCCGCTA CAGGGGGGGCCTGAGGCACTGCAGAAAGTGGGCCTGAGCCTCGAGGATGACGGTGCTGCA GGAACCCGTCCAGGCTGCTATATGGCAAGCACTAAACCACTATGCTTACCGAGATGCGGT TTTCCTCGCAGAACGCCTTTATGCAGAAGTACACTCAGAAGAAGCCTTGTTTTTACTGGC AACCTGTTATTACCGCTCAGGAAAGGCATATAAAGCATATAGACTCTTGAAAGGACACAG TTGTACTACACCGCAATGCAAATACCTGCTTGCAAAATGTTGTGTTGATCTCAGCAAGCT TGCAGAAGGGGAACAAATCTTATCTGGTGGAGTGTTTAATAAGCAGAAAAGCCATGATGA TATTGTTACTGAGTTTGGTGATTCAGCTTGCTTTACTCTTTCATTGTTGGGACATGTATA TTGCAAGACAGATCGGCTTGCCAAAGGATCAGAATGTTACCAAAAGAGCCTTAGTTTAAA TCCTTTCCTCTGGTCTCCCTTTGAATCATTATGTGAAATAGGTGAAAAGCCAGATCCTGA CCAAACATTTAAATTCACATCTTTACAGAACTTTAGCAACTGTCTGCCCAACTCTTGCAC AACACAAGTACCTAATCATAGTTTATCTCACAGACAGCCTGAGACAGTTCTTACGGAAAC ACCCCAGGACACAATTGAATTAAACAGATTGAATTTAGAATCTTCCAATTCAAAGTACTC CTTGAATACAGATTCCTCAGTGTCTTATATTGATTCAGCTGTAATTTCACCTGATACTGT CCCACTGGGAACAGGAACTTCCATATTATCTAAACAGGTTCAAAATAAACCAAAAACTGG TCGAAGTTTATTAGGAGGACCAGCAGCTCTTAGTCCATTAACCCCAAGTTTTGGGATTTT GCCATTAGAAACCCCAAGTCCTGGAGATGGATCCTATTTACAAAACTACACTAATACACC TCCTGTAATTGATGTGCCATCCACCGGAGCCCCTTCAAAAAAGACTTTTCGTGTTTTACA GTCTGTTGCCAGAATCGGCCAAACTGGAACAAAGTCTGTCTTCTCACAGAGTGGAAATAG CCGAGAGGTAACTCCAATTCTTGCACAAACACAAAGTTCTGGTCCACAAACAAGTACAAC ACCTCAGGTATTGAGCCCCACTATTACATCTCCCCCAAACGCACTGCCTCGAAGAAGTTC ACGACTCTTTACTAGTGACAGCTCCACAACCAAGGAGAATAGCAAAAAATTAAAAATGAA GTTTCCACCTAAAATCCCAAACAGAAAAACAAAAAGTAAAACTAATAAAGGAGGAATAAC TCAACCTAACATAAATGATAGCCTGGAAATTACAAAATTGGACTCTTCCATCATTTCAGA AGGGAAAATATCCACAATCACACCTCAGATTCAGGCCTTTAATCTACAAAAAGCAGCAGC AGAAGGTTTGATGAGCCTTCTTCGTGAAATGGGGAAAGGTTATTTAGCTTTGTGTTCATA CAACTGCAAAGAAGCTATAAATATTTTGAGCCATCTACCTTCTCACCACTACAATACTGG TTGGGTACTGTGCCAAATTGGAAGGGCCTATTTTGAACTTTCAGAGTACATGCAAGCTGA AAGAATATTCTCAGAGGTTAGAAGGATTGAGAATTATAGAGTTGAAGGCATGGAGATCTA CTCTACAACACTTTGGCATCTTCAAAAAGATGTTGCTCTTTCAGTTCTGTCAAAAGACTT AACAGACATGGATAAAAATTCGCCAGAGGCCTGGTGTGCTGCAGGGAACTGTTTCAGTCT GCAACGGGAACATGATATTGCAATTAAATTCTTCCAGAGAGCTATCCAAGTTGATCCAAA TTACGCTTATGCCTATACTCTATTAGGGCATGAGTTTGTCTTAACTGAAGAATTGGACAA AGCATTAGCTTGTTTTCGAAATGCTATCAGAGTCAATCCTAGACATTATAATGCATGGTA TGGTTTAGGAATGATTTATTACAAGCAAGAAAAATTCAGCCTTGCAGAAATGCATTTCCA AAAAGCGCTTGATATCAACCCTCAAAGTTCAGTTTTACTTTGCCACATTGGAGTAGTTCA ACATGCACTGAAAAAATCAGAGAAGGCTTTGGATACCCTAAACAAAGCCATTGTCATTGA TCCCAAGAACCCTCTATGCAAATTTCACAGAGCCTCAGTTTTATTTGCAAATGAAAAATA TAAGTCTGCTTTACAAGAACTTGAAGAATTGAAACAAATTGTTCCCAAAGAATCCCTCGT TTACTTCTTAATAGGAAAGGTTTACAAGAAGTTAGGTCAAACGCACCTCGCCCTGATGAA TTTCTCTTGGGCTATGGATTTAGATCCTAAAGGAGCCAATAACCAGATTAAAGAGGCAAT TGATAAGCGTTATCTTCCAGATGATGAGGAGCCAATAACCCAAGAAGAACAGATCATGGG AACAGATGAATCCCAGGAGAGCAGCATGACAGATGCGGATGACACACAACTTCATGCAGC TGAAAGTGATGAATTTTAACTTCTGGAAATCAGACTTTTACAACTGGATGTGTGACTAGT GCTGACGTGTTTCTTGTCCCTCTGTATACTGAGTCTTTACTCTTGAGCTGGCGGTGTCAT CGTCCGTCACTTATACCATGAGTGTGCCACTTTCATTGGACCCTGACTGTATACAGAATG AAAGGCAGTGCAATATTTAGCTGCTAACAAGACTGGCTCTTTTACCAGTATGAATGACAA TTTATGGGGGGTAGGGTGGGGAACTTTCTTTTCTGTTTTTCTTTAATCTCCCTTTGTTGG AAAGTATCATGAAAGGAAGAGTTATGCTTTATCTTGAAGGAACCATTAGATATGGAAAAT AGTGATGAACCAGAGTTTCTTGGTTGCTTTTTCAAAAATTTGTTTTTATTTGGTTCTGTT CCTGATAAACAGAGTAACTGACCTTCATTTCTAGGTTCTTCAAGAATGGTGTTTGCAAGT GCCAGATGGAACAATAAAAGACGTTGCCTATAACAGTGAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_001114091 |
| Insert Size | 3100 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The ORF of this clone has been fully sequenced and found to be a perfect match to NM_001114091.1. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001114091.1, NP_001107563.1 |
| RefSeq Size | 5611 bp |
| RefSeq ORF | 2493 bp |
| Locus ID | 996 |
| UniProt ID | P30260 |
| Protein Families | Druggable Genome |
| Protein Pathways | Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis |
| Gene Summary | The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y. [provided by RefSeq, May 2014] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226198 | CDC27 (Myc-DDK-tagged)-Human cell division cycle 27 homolog (S. cerevisiae) (CDC27), transcript variant 1 |
CNY 6,080.00 |
|
| RC226198L1 | Lenti ORF clone of Human cell division cycle 27 homolog (S. cerevisiae) (CDC27), transcript variant 1, Myc-DDK-tagged |
CNY 8,480.00 |
|
| RC226198L2 | Lenti ORF clone of Human cell division cycle 27 homolog (S. cerevisiae) (CDC27), transcript variant 1, mGFP tagged |
CNY 10,640.00 |
|
| RC226198L3 | Lenti ORF clone of Human cell division cycle 27 homolog (S. cerevisiae) (CDC27), transcript variant 1, Myc-DDK-tagged |
CNY 10,640.00 |
|
| RC226198L4 | Lenti ORF clone of Human cell division cycle 27 homolog (S. cerevisiae) (CDC27), transcript variant 1, mGFP tagged |
CNY 10,640.00 |
|
| RG226198 | CDC27 (tGFP-tagged) - Human cell division cycle 27 homolog (S. cerevisiae) (CDC27), transcript variant 1 |
CNY 9,600.00 |
