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ARHGEF7 (NM_001113513) Human Untagged Clone
CAT＃: SC318960
ARHGEF7 (untagged)-Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 5
CNY 10,360.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BETA-PIX; COOL-1; COOL1; Nbla10314; P50; P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC318960 representing NM_001113513.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACCGATAATAGCAACAATCAACTGGTAGTAAGAGCAAAGTTTAACTTCCAGCAGACCAATGAGGAC GAGCTTTCCTTCTCAAAAGGAGACGTCATCCATGTCACCCGTGTGGAAGAGGGAGGCTGGTGGGAGGGC ACACTCAACGGCCGGACCGGCTGGTTCCCCAGCAACTACGTGCGCGAGGTCAAGGCCAGCGAGAAGCCT GTGTCTCCCAAATCAGGAACACTGAAGAGCCCTCCCAAAGGATTTGATACGACTGCCATAAACAAAAGC TATTACAATGTGGTGCTACAGAATATTTTAGAAACAGAAAATGAATATTCTAAAGAACTTCAGACTGTG CTTTCAACGTACCTACGGCCATTGCAGACCAGTGAGAAGTTAAGTTCAGCAAACATTTCATATTTAATG GGAAATCTAGAAGAAATATGTTCTTTCCAGCAAATGCTCGTACAGTCTTTAGAAGAATGCACCAAGTTG CCCGAAGCTCAGCAGAGAGTCGGAGGCTGCTTTTTAAACCTGATGCCACAGATGAAAACCCTGTACCTC ACGTATTGTGCCAATCACCCTTCTGCAGTGAATGTCCTCACGGAACACAGTGAGGAGTTGGGGGAGTTC ATGGAGACCAAAGGTGCCAGCAGCCCTGGGATTCTCGTGCTGACCACGGGCCTGAGCAAACCCTTCATG CGCCTGGATAAATACCCTACGCTGCTCAAAGAGCTCGAGAGACACATGGAGGATTATCATACAGATAGA CAAGATATTCAAAAATCCATGGCTGCCTTCAAAAACCTTTCAGCCCAATGTCAAGAAGTCCGGAAGAGG AAAGAGCTTGAGCTGCAGATCCTGACGGAAGCCATCCGGAACTGGGAGGGCGATGACATTAAAACTCTG GGCAACGTCACTTACATGTCCCAGGTCCTGATTCAGTGTGCCGGAAGTGAGGAAAAGAATGAAAGATAT CTTCTACTCTTCCCAAATGTTTTGCTAATGTTGTCTGCCAGTCCTAGGATGAGTGGCTTTATCTATCAG GGAAAGCTTCCAACGACAGGAATGACAATCACAAAGCTTGAGGACAGTGAAAATCATAGAAATGCATTT GAAATATCAGGGAGCATGATTGAGCGGATATTAGTGTCGTGCAACAACCAGCAGGATCTGCAGGAATGG GTGGAGCACCTACAGAAGCAAACGAAGGTCACGTCTGTGGGAAACCCCACCATAAAGCCTCATTCAGTG CCATCTCATACCCTCCCCTCCCACCCGGTCACTCCGTCCAGCAAGCACGCAGACAGCAAGCCCGCGCCG CTGACGCCCGCCTACCACACGCTGCCCCACCCCTCCCACCACGGCACCCCGCACACCACCATCAACTGG GGACCCCTGGAGCCTCCGAAAACACCCAAGCCCTGGAGCCTGAGCTGCCTGCGGCCCGCGCCTCCCCTC CGGCCCTCAGCTGCTCTCTGCTACAAGGAGGATCTTAGTAAGAGCCCTAAGACCATGAAAAAGCTGCTG CCCAAGCGCAAACCTGAACGGAAGCCTTCAGATGAGGAGTTCGCGTCCCGGAAAAGCACAGCTGCTTTG GAAGAAGATGCTCAGATTCTGAAAGTCATTGAAGCTTACTGCACCAGCGCCAAAACAAGGCAAACACTC AATTCAAGTTCACGCAAAGAATCTGCTCCACAAGTTTTGCTTCCAGAAGAAGAGAAAATTATAGTGGAA GAAACTAAAAGTAATGGTCAGACAGTGATAGAAGAAAAGAGTCTTGTGGATACCGTATATGCATTAAAG GATGAAGTTCAAGAATTAAGACAGGACAACAAAAAGATGAAGAAATCTCTAGAGGAAGAACAGAGAGCC CGCAAAGACCTGGAGAAGCTGGTGAGGAAAGTCCTGAAGAACATGAATGATCCTGCCTGGGATGAGACC AATCTATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001113513 |
| Insert Size | 1941 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001113513.1 |
| RefSeq Size | 4956 bp |
| RefSeq ORF | 1941 bp |
| Locus ID | 8874 |
| UniProt ID | Q14155 |
| Protein Pathways | Regulation of actin cytoskeleton |
| MW | 73.1 kDa |
| Gene Summary | This gene encodes a protein that belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It forms a complex with the small GTP binding protein Rac1 and recruits Rac1 to membrane ruffles and to focal adhesions. Multiple alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Mar 2016] Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region, and contains a different 3' coding region and 3' UTR, compared to variant 1. It initiates translation at a downstream start codon. Variants 1, 5, 6, 18, and 19 all encode isoform a (also known as p85Cool-1), which has a shorter N-terminus and a longer and distinct C-terminus, compared to isoform c. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226057 | ARHGEF7 (Myc-DDK-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 5 |
CNY 4,560.00 |
|
| RC226057L3 | Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 5, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC226057L4 | Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 5, mGFP tagged |
CNY 6,460.00 |
|
| RG226057 | ARHGEF7 (tGFP-tagged) - Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 5 |
CNY 5,040.00 |
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