United States
Germany
Japan
United Kingdom
China
TJP1 (NM_003257) Human Untagged Clone
CAT＃: SC318666
TJP1 (untagged)-Human tight junction protein 1 (zona occludens 1) (TJP1), transcript variant 1
CNY 26,790.00
货期*
4周
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ZO-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC318666 representing NM_003257.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCCGCCAGAGCTGCGGCCGCCAAGAGCACAGCAATGGAGGAAACAGCTATATGGGAACAACATACA GTGACGCTTCACAGGGCTCCTGGATTTGGATTTGGAATTGCAATATCTGGTGGACGAGATAATCCTCAT TTTCAGAGTGGGGAAACGTCAATAGTGATTTCAGATGTGCTGAAAGGAGGACCAGCTGAAGGACAGCTA CAGGAAAATGACCGAGTTGCAATGGTTAACGGAGTTTCAATGGATAATGTTGAACATGCTTTTGCTGTT CAGCAACTAAGGAAAAGTGGGAAAAATGCAAAAATTACAATTAGAAGGAAGAAGAAAGTTCAAATACCA GTAAGTCGTCCTGATCCTGAACCAGTATCTGATAATGAAGAAGATAGTTATGATGAGGAAATACATGAT CCAAGAAGTGGCCGGAGTGGTGTGGTTAACAGAAGGAGTGAGAAGATTTGGCCGAGGGATAGAAGTGCA AGTAGAGAGAGGAGCTTGTCCCCGCGGTCAGACAGGCGGTCAGTGGCTTCCAGCCAGCCTGCTAAACCT ACTAAAGTCACACTGGTGAAATCCCGGAAAAATGAAGAATATGGTCTTCGATTGGCAAGCCATATATTT GTTAAGGAAATTTCACAAGATAGTTTGGCAGCAAGAGATGGCAATATTCAAGAAGGTGATGTTGTATTG AAGATAAATGGTACTGTGACAGAAAATATGTCATTGACAGATGCAAAGACATTGATAGAAAGGTCTAAA GGCAAATTAAAAATGGTAGTTCAAAGAGATGAACGGGCTACGCTATTGAATGTCCCTGATCTTTCTGAC AGCATCCACTCTGCTAATGCCTCTGAGAGAGACGACATTTCAGAAATTCAGTCACTGGCATCAGATCAT TCTGGTCGATCACACGATAGGCCTCCCCGCCGCAGCCGGTCACGATCTCCTGACCAGCGGTCAGAGCCT TCTGATCATTCCAGGCACTCGCCGCAGCAGCCAAGCAATGGCAGTCTCCGGAGTAGAGATGAAGAGAGA ATTTCTAAACCTGGGGCTGTCTCAACTCCTGTAAAGCATGCTGATGATCACACACCTAAAACAGTGGAA GAAGTTACAGTTGAAAGAAATGAGAAACAAACACCTTCTCTTCCAGAACCAAAGCCTGTGTATGCCCAA GTTGGGCAACCAGATGTGGATTTACCTGTCAGTCCATCTGATGGTGTCCTACCTAATTCAACTCATGAA GATGGGATTCTTCGGCCCAGCATGAAATTGGTAAAATTCAGAAAAGGAGATAGTGTGGGTTTGCGGCTG GCTGGTGGAAATGATGTTGGAATATTTGTAGCTGGCGTTCTAGAAGATAGCCCTGCAGCCAAGGAAGGC TTAGAGGAAGGTGATCAAATTCTCAGGGTAAACAACGTAGATTTTACAAATATCATAAGAGAAGAAGCC GTCCTTTTCCTGCTTGACCTCCCTAAAGGAGAAGAAGTGACCATATTGGCTCAGAAGAAGAAGGATGTT TATCGTCGCATTGTAGAATCAGATGTAGGAGATTCTTTCTATATTAGAACCCATTTTGAATATGAAAAG GAATCTCCCTATGGACTTAGTTTTAACAAAGGAGAGGTGTTCCGTGTTGTGGATACCTTGTACAATGGA AAACTGGGCTCTTGGCTTGCTATTCGAATTGGTAAAAATCATAAGGAGGTAGAACGAGGCATCATCCCT AATAAGAACAGAGCTGAGCAGCTAGCCAGTGTACAGTATACACTTCCAAAAACAGCAGGCGGAGACCGT GCTGACTTCTGGAGATTCAGAGGTCTTCGCAGCTCCAAGAGAAATCTTCGAAAAAGCAGAGAGGATTTG TCCGCTCAGCCTGTTCAAACAAAGTTTCCAGCTTATGAAAGAGTGGTTCTTCGAGAAGCTGGATTTCTG AGGCCTGTAACCATTTTTGGACCAATAGCTGATGTTGCCAGAGAAAAGCTGGCAAGAGAAGAACCAGAT ATTTATCAAATTGCAAAGAGTGAACCACGAGACGCTGGAACTGACCAACGTAGCTCTGGCATTATTCGC CTGCATACAATAAAGCAAATCATAGATCAAGACAAACATGCTTTATTAGATGTAACACCAAATGCAGTT GATCGTCTTAACTATGCCCAGTGGTATCCAATTGTTGTATTTCTTAACCCTGATTCTAAGCAAGGAGTA AAAACAATGAGAATGAGGTTATGTCCAGAATCTCGGAAAAGTGCCAGGAAGTTATACGAGCGATCTCAT AAACTTCGTAAAAATAATCACCATCTTTTTACAACTACAATTAACTTAAATTCAATGAATGATGGTTGG TATGGTGCGCTGAAAGAAGCAATTCAACAACAGCAAAACCAGCTGGTATGGGTTTCCGAGGGAAAGGCG GATGGTGCTACAAGTGATGACCTTGATTTGCATGATGATCGTCTGTCCTACCTGTCAGCTCCAGGTAGT GAATACTCAATGTATAGCACGGACAGTAGACACACTTCTGACTATGAAGACACAGACACAGAAGGCGGG GCCTACACTGATCAAGAACTAGATGAAACTCTTAATGATGAGGTTGGGACTCCACCGGAGTCTGCCATT ACACGGTCCTCTGAGCCTGTAAGAGAGGACTCCTCTGGAATGCATCATGAAAACCAAACATATCCTCCT TACTCACCACAAGCGCAGCCACAACCAATTCATAGAATAGACTCCCCTGGATTTAAGCCAGCCTCTCAA CAGAAAGCAGAAGCTTCATCTCCAGTCCCTTACCTTTCGCCTGAAACAAACCCAGCATCATCAACCTCT GCTGTTAATCATAATGTAAATTTAACTAATGTCAGACTGGAGGAGCCCACCCCAGCTCCTTCCACCTCT TACTCACCACAAGCTGATTCTTTAAGAACACCAAGTACTGAGGCAGCTCACATAATGCTAAGAGATCAA GAACCATCATTGTCGTCGCATGTAGATCCAACAAAGGTGTATAGAAAGGATCCATATCCCGAGGAAATG ATGAGGCAGAACCATGTTTTGAAACAGCCAGCCGTTAGTCACCCAGGGCACAGGCCAGACAAAGAGCCT AATCTGACCTATGAACCCCAACTCCCATACGTAGAGAAACAAGCCAGCAGAGACCTCGAGCAGCCCACA TACAGATACGAGTCCTCAAGCTATACGGACCAGTTTTCTCGAAACTATGAACATCGTCTGCGATACGAA GATCGCGTCCCCATGTATGAAGAACAGTGGTCATATTATGATGACAAACAGCCCTACCCATCTCGGCCA CCTTTTGATAATCAGCACTCTCAAGACCTTGACTCCAGACAGCATCCCGAAGAGTCCTCAGAACGAGGG TACTTTCCACGTTTTGAAGAGCCAGCCCCTCTGTCTTACGACAGCAGACCACGTTACGAACAGGCACCT AGAGCATCCGCCCTGCGGCACGAAGAGCAGCCAGCTCCTGGGTATGACACACATGGTAGACTCAGACCG GAAGCCCAGCCCCACCCTTCAGCAGGGCCCAAGCCTGCAGAGTCCAAGCAGTATTTTGAGCAATATTCA CGCAGTTACGAGCAAGTACCACCCCAAGGATTTACCTCTAGAGCAGGTCATTTTGAGCCTCTCCATGGT GCTGCAGCTGTCCCTCCGCTGATACCTTCATCTCAGCATAAGCCAGAAGCTCTGCCTTCAAACACCAAA CCACTGCCTCCACCCCCAACTCAAACCGAAGAAGAGGAAGATCCAGCAATGAAGCCACAGTCTGTACTC ACCAGAGTTAAGATGTTTGAAAACAAAAGATCTGCATCCTTAGAGACCAAGAAGGATGTAAATGACACT GGCAGTTTTAAGCCTCCAGAAGTAGCATCTAAACCTTCAGGTGCTCCCATCATTGGTCCCAAACCCACT TCTCAGAATCAATTCAGTGAACATGACAAAACTCTGTACAGGATCCCAGAACCTCAAAAACCTCAACTG AAGCCACCTGAAGATATTGTTCGGTCCAATCATTATGACCCTGAAGAAGATGAAGAATATTATCGAAAA CAGCTGTCATACTTTGACCGAAGAAGTTTTGAGAATAAGCCTCCTGCACACATTGCCGCCAGCCATCTC TCCGAGCCTGCAAAGCCAGCGCATTCTCAGAATCAATCAAATTTTTCTAGTTATTCTTCAAAGGGAAAG CCTCCTGAAGCTGATGGTGTGGATAGATCATTTGGCGAGAAACGCTATGAACCCATCCAGGCCACTCCC CCTCCTCCTCCATTGCCCTCGCAGTATGCCCAGCCATCTCAGCCTGTCACCAGCGCGTCTCTCCACATA CATTCTAAGGGAGCACATGGTGAAGGTAATTCAGTGTCATTGGATTTTCAGAATTCCTTAGTGTCCAAA CCAGACCCACCTCCATCTCAGAATAAGCCAGCAACTTTCAGACCACCAAACCGAGAAGATACTGCTCAG GCAGCTTTCTATCCCCAGAAAAGTTTTCCAGATAAAGCCCCAGTTAATGGAACTGAACAGACTCAGAAA ACAGTCACTCCAGCATACAATCGATTCACACCAAAACCATATACAAGTTCTGCCCGACCATTTGAACGC AAGTTTGAAAGTCCTAAATTCAATCACAATCTTCTGCCAAGTGAAACTGCACATAAACCTGACTTGTCT TCAAAAACTCCCACTTCTCCAAAAACTCTTGTGAAATCGCACAGTTTGGCACAGCCTCCTGAGTTTGAC AGTGGAGTTGAAACTTTCTCTATCCATGCAGAGAAGCCTAAATATCAAATAAATAATATCAGCACAGTG CCTAAAGCTATTCCTGTGAGTCCTTCAGCTGTGGAAGAGGATGAAGATGAAGATGGTCATACTGTGGTG GCCACAGCCCGAGGCATATTTAACAGCAATGGGGGCGTGCTGAGTTCCATAGAAACTGGTGTTAGTATA ATTATCCCTCAAGGAGCCATTCCCGAAGGAGTTGAGCAGGAAATCTATTTCAAGGTCTGCCGGGACAAC AGCATCCTTCCACCTTTAGATAAAGAGAAAGGTGAAACACTGCTGAGTCCTTTGGTGATGTGTGGTCCC CATGGCCTCAAGTTCCTGAAGCCTGTGGAGCTGCGCTTACCACACTGTGATCCTAAAACCTGGCAAAAC AAGTGTCTTCCCGGAGATCCAAATTATCTCGTTGGAGCAAACTGTGTTTCTGTCCTTATTGACCACTTT TAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_003257 |
| Insert Size | 5247 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003257.4 |
| RefSeq Size | 7971 bp |
| RefSeq ORF | 5247 bp |
| Locus ID | 7082 |
| UniProt ID | Q07157 |
| Domains | ZU5, PDZ, Guanylate_kin, GuKc |
| Protein Families | Druggable Genome |
| Protein Pathways | Adherens junction, Epithelial cell signaling in Helicobacter pylori infection, Gap junction, Tight junction, Vibrio cholerae infection |
| MW | 195.5 kDa |
| Gene Summary | This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017] Transcript Variant: This variant (1, also known as ZO-1alpha+) encodes isoform a, which is found in most epithelial cell junctions. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222836 | TJP1 (Myc-DDK-tagged)-Human tight junction protein 1 (zona occludens 1) (TJP1), transcript variant 1 |
CNY 17,624.00 |
|
| RC222836L1 | Lenti ORF clone of Human tight junction protein 1 (zona occludens 1) (TJP1), transcript variant 1, Myc-DDK-tagged |
CNY 20,024.00 |
|
| RC222836L3 | Lenti ORF clone of Human tight junction protein 1 (zona occludens 1) (TJP1), transcript variant 1, Myc-DDK-tagged |
CNY 20,024.00 |
|
| RG222836 | TJP1 (tGFP-tagged) - Human tight junction protein 1 (zona occludens 1) (TJP1), transcript variant 1 |
CNY 19,224.00 |
|
| SC127835 | TJP1 (untagged)-Human tight junction protein 1 (zona occludens 1) (TJP1), transcript variant 1 |
CNY 17,512.00 |
Customer
Reviews
Loading...
