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TJP1 (NM_003257) Human Tagged Lenti ORF Clone
CAT＃: RC222836L1
- LentiORF®
Lenti ORF clone of Human tight junction protein 1 (zona occludens 1) (TJP1), transcript variant 1, Myc-DDK-tagged
"NM_003257" in other vectors (5)
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CNY 20,024.00
Product images
CNY 600.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ZO-1 |
| Vector | pLenti-C-Myc-DDK |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
The ORF insert of this clone is exactly the same as(RC222836).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003257 |
| ORF Size | 5244 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003257.3 |
| RefSeq Size | 7165 bp |
| RefSeq ORF | 5247 bp |
| Locus ID | 7082 |
| UniProt ID | Q07157 |
| Domains | ZU5, PDZ, Guanylate_kin, GuKc |
| Protein Families | Druggable Genome |
| Protein Pathways | Adherens junction, Epithelial cell signaling in Helicobacter pylori infection, Gap junction, Tight junction, Vibrio cholerae infection |
| MW | 195.3 kDa |
| Gene Summary | This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222836 | TJP1 (Myc-DDK-tagged)-Human tight junction protein 1 (zona occludens 1) (TJP1), transcript variant 1 |
CNY 17,624.00 |
|
| RC222836L3 | Lenti ORF clone of Human tight junction protein 1 (zona occludens 1) (TJP1), transcript variant 1, Myc-DDK-tagged |
CNY 20,024.00 |
|
| RG222836 | TJP1 (tGFP-tagged) - Human tight junction protein 1 (zona occludens 1) (TJP1), transcript variant 1 |
CNY 19,224.00 |
|
| SC127835 | TJP1 (untagged)-Human tight junction protein 1 (zona occludens 1) (TJP1), transcript variant 1 |
CNY 17,512.00 |
|
| SC318666 | TJP1 (untagged)-Human tight junction protein 1 (zona occludens 1) (TJP1), transcript variant 1 |
CNY 26,790.00 |
