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GLI2 (NM_005270) Human Untagged Clone
CAT＃: SC318642
GLI2 (untagged)-Human GLI family zinc finger 2 (GLI2)
CNY 30,590.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CJS; HPE9; PHS2; THP1; THP2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC318642 representing NM_005270.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGACGTCTGCCTCAGCCACTGCCTCCGAGAAGCAAGAAGCCAAAAGTGGGATCCTGGAGGCCGCT GGCTTCCCCGACCCGGGTAAAAAGGCCTCTCCTTTGGTGGTGGCTGCAGCGGCAGCAGCAGCGGTAGCT GCCCAAGGAGTGCCGCAGCATCTCTTGCCACCATTCCATGCGCCCCTACCGATTGACATGCGACACCAG GAAGGAAGGTACCATTACGAGCCTCATTCTGTCCACGGTGTGCACGGGCCCCCTGCCCTCAGCGGCAGC CCTGTCATCTCTGACATCTCCTTGATCCGGCTTTCCCCGCACCCGGCTGGCCCTGGGGAGTCCCCCTTC AACGCCCCCCACCCGTACGTGAACCCCCACATGGAGCACTACCTCCGTTCTGTGCACAGCAGCCCCACG CTCTCCATGATCTCTGCAGCCAGGGGCCTCAGCCCCGCTGATGTGGCCCAGGAGCACCTTAAGGAGAGG GGACTGTTTGGCCTTCCTGCTCCAGGCACCACCCCCTCAGACTATTACCACCAGATGACCCTCGTGGCA GGCCACCCCGCGCCCTACGGGGACCTGCTGATGCAGAGCGGGGGCGCTGCCAGCGCACCCCATCTCCAC GACTACCTCAACCCCGTGGACGTGTCCCGTTTCTCCAGCCCGCGGGTGACGCCCCGCCTGAGCCGCAAG CGGGCGCTGTCCATCTCCCCACTCTCAGACGCCAGCCTGGACCTGCAGCGGATGATCCGCACCTCACCC AACTCGCTAGTGGCCTACATCAACAACTCCCGAAGCAGCTCGGCGGCCAGCGGTTCCTACGGGCATCTG TCAGCGGGTGCCCTCAGCCCAGCCTTCACCTTCCCCCACCCCATCAACCCCGTGGCCTACCAGCAGATT CTGAGCCAGCAGAGGGGTCTGGGGTCAGCCTTTGGACACACACCACCCCTGATCCAGCCCTCACCCACC TTCCTGGCCCAGCAGCCCATGGCCCTCACCTCCATCAATGCCACGCCCACCCAGCTCAGCAGCAGCAGC AACTGTCTGAGTGACACCAACCAGAACAAGCAGAGCAGTGAGTCGGCCGTCAGCAGCACCGTCAACCCT GTCGCCATTCACAAGCGCAGCAAGGTCAAGACCGAGCCTGAGGGCCTGCGGCCGGCCTCCCCTCTGGCG CTGACGCAGGGCCAGGTGTCTGGACACGGCTCATGTGGGTGTGCCCTTCCCCTCTCCCAGGAGCAGCTG GCTGACCTCAAGGAAGATCTGGACAGGGATGACTGTAAGCAGGAGGCTGAGGTGGTCATCTATGAGACC AACTGCCACTGGGAAGACTGCACCAAGGAGTACGACACCCAGGAGCAGCTGGTGCATCACATCAACAAC GAGCACATCCACGGGGAGAAGAAGGAGTTTGTGTGCCGCTGGCAGGCCTGCACGCGGGAGCAGAAGCCC TTCAAGGCGCAGTACATGCTGGTGGTGCACATGCGGCGACACACGGGCGAGAAGCCCCACAAGTGCACG TTCGAGGGCTGCTCGAAGGCCTACTCCCGCCTGGAGAACCTGAAGACACACCTGCGGTCCCACACCGGG GAGAAGCCATATGTGTGTGAGCACGAGGGCTGCAACAAAGCCTTCTCCAACGCCTCGGACCGCGCCAAG CACCAGAATCGCACCCACTCCAACGAGAAACCCTACATCTGCAAGATCCCAGGCTGCACCAAGAGATAC ACAGACCCCAGCTCTCTCCGGAAGCATGTGAAAACGGTCCACGGCCCAGATGCCCACGTCACCAAGAAG CAGCGCAATGACGTGCACCTCCGCACACCGCTGCTCAAAGAGAATGGGGACAGTGAGGCCGGCACGGAG CCTGGCGGCCCAGAGAGCACCGAGGCCAGCAGCACCAGCCAGGCCGTGGAGGACTGCCTGCACGTCAGA GCCATCAAGACCGAGAGCTCCGGGCTGTGTCAGTCCAGCCCCGGGGCCCAGTCGTCCTGCAGCAGCGAG CCCTCTCCTCTGGGCAGTGCCCCCAACAATGACAGTGGCGTGGAGATGCCGGGGACGGGGCCCGGGAGC CTGGGAGACCTGACGGCACTGGATGACACACCCCCAGGGGCCGACACCTCAGCCCTGGCTGCCCCCTCC GCTGGTGGCCTCCAGCTGCGCAAACACATGACCACCATGCACCGGTTCGAGCAGCTCAAGAAGGAGAAG CTCAAGTCACTCAAGGATTCCTGCTCATGGGCCGGGCCGACTCCACACACGCGGAACACCAAGCTGCCT CCCCTCCCGGGAAGTGGCTCCATCCTGGAAAACTTCAGTGGCAGTGGGGGCGGCGGGCCCGCGGGGCTG CTGCCGAACCCGCGGCTGTCGGAGCTGTCCGCGAGCGAGGTGACCATGCTGAGCCAGCTGCAGGAGCGC CGCGACAGCTCCACCAGCACGGTCAGCTCGGCCTACACCGTGAGCCGCCGCTCCTCCGGCATCTCCCCC TACTTCTCCAGCCGCCGCTCCAGCGAGGCCTCGCCCCTGGGCGCCGGCCGCCCGCACAACGCGAGCTCC GCTGACTCCTACGACCCCATCTCCACGGACGCGTCGCGGCGCTCGAGCGAGGCCAGCCAGTGCAGCGGC GGCTCCGGGCTGCTCAACCTCACGCCGGCGCAGCAGTACAGCCTGCGGGCCAAGTACGCGGCAGCCACT GGCGGCCCCCCGCCCACTCCGCTGCCGGGCCTGGAGCGCATGAGCCTGCGGACCAGGCTGGCGCTGCTG GACGCGCCCGAGCGCACGCTGCCCGCCGGCTGCCCACGCCCACTGGGGCCGCGGCGTGGCAGCGACGGG CCGACCTATGGCCACGGCCACGCGGGGGCTGCGCCCGCCTTCCCCCACGAGGCTCCAGGCGGCGGAGCC AGGCGGGCCAGCGACCCTGTGCGGCGGCCCGATGCCCTGTCCCTGCCGCGGGTGCAGCGCTTCCACAGC ACCCACAACGTGAACCCCGGCCCGCTGCCGCCCTGTGCCGACAGGCGAGGCCTCCGCCTGCAGAGCCAC CCGAGCACCGACGGCGGCCTGGCCCGCGGCGCCTACTCGCCCCGGCCGCCTAGCATCAGCGAGAACGTG GCGATGGAGGCCGTGGCGGCAGGAGTGGACGGCGCGGGGCCCGAGGCCGACCTGGGGCTGCCGGAGGAC GACCTGGTGCTTCCAGACGACGTGGTGCAGTACATCAAGGCGCACGCCAGTGGCGCTCTGGACGAGGGC ACCGGGCAGGTGTATCCCACGGAAAGCACTGGCTTCTCTGACAACCCCAGACTACCCAGCCCGGGGCTG CACGGCCAGCGCAGGATGGTGGCTGCGGACTCCAACGTGGGCCCCTCCGCCCCTATGCTGGGAGGATGC CAGTTAGGCTTTGGGGCGCCCTCCAGCCTGAACAAAAATAACATGCCTGTGCAGTGGAATGAGGTGAGC TCCGGCACCGTAGACGCCCTGGCCAGCCAGGTGAAGCCTCCACCCTTTCCTCAGGGCAACCTGGCGGTG GTGCAGCAGAAGCCTGCCTTTGGCCAGTACCCGGGCTACAGTCCGCAAGGCCTACAGGCTAGCCCTGGG GGCCTGGACAGCACGCAGCCACACCTGCAGCCCCGCAGCGGAGCCCCCTCCCAGGGCATCCCCAGGGTA AACTACATGCAGCAGCTGCGACAGCCAGTGGCAGGCAGCCAGTGTCCTGGCATGACTACCACTATGAGC CCCCATGCCTGCTATGGCCAAGTCCACCCCCAGCTGAGCCCCAGCACCATCAGTGGGGCCCTCAACCAG TTCCCCCAATCCTGCAGCAACATGCCAGCCAAGCCAGGGCATCTGGGGCACCCTCAGCAGACAGAAGTG GCACCTGACCCCACCACGATGGGCAATCGCCACAGGGAACTTGGGGTCCCCGATTCAGCCCTGGCTGGA GTGCCACCACCTCACCCAGTCCAGAGCTACCCACAGCAGAGCCATCACCTGGCAGCCTCCATGAGCCAG GAGGGCTACCACCAGGTCCCCAGCCTTCTGCCTGCCCGCCAGCCTGGCTTCATGGAGCCCCAAACAGGC CCGATGGGGGTGGCTACAGCAGGCTTTGGCCTAGTGCAGCCCCGGCCTCCCCTCGAGCCCAGCCCCACT GGCCGCCACCGTGGGGTACGTGCTGTGCAGCAGCAGCTGGCCTACGCCAGGGCCACAGGCCATGCCATG GCTGCCATGCCGTCCAGTCAGGAAACAGCAGAGGCTGTGCCCAAGGGAGCGATGGGCAACATGGGGTCG GTGCCTCCCCAGCCGCCTCCGCAGGACGCAGGTGGGGCCCCGGACCACAGCATGCTCTACTACTACGGC CAGATCCACATGTACGAACAGGATGGAGGCCTGGAGAACCTCGGGAGCTGCCAGGTCATGCGGTCCCAG CCACCACAGCCACAGGCCTGTCAGGACAGCATCCAGCCCCAGCCCTTGCCCTCACCAGGGGTCAACCAG GTGTCCAGCACTGTGGACTCCCAGCTCCTGGAGGCCCCCCAGATTGACTTCGATGCCATCATGGATGAT GGCGATCACTCGAGTTTGTTCTCGGGTGCTCTGAGCCCCAGCCTCCTCCACAGCCTCTCCCAGAACTCC TCCCGCCTCACCACCCCCCGAAACTCCTTGACCCTGCCCTCCATCCCCGCAGGCATCAGCAACATGGCT GTCGGGGACATGAGCTCCATGCTCACCAGCCTCGCCGAGGAGAGCAAGTTCCTGAACATGATGACCTAG ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAG GATGACGACGATAAGGTTTAAACGGCCGGCCGCGGT |
| Restriction Sites | SgfI-NotI |
| ACCN | NM_005270 |
| Insert Size | 4761 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005270.4 |
| RefSeq Size | 6780 bp |
| RefSeq ORF | 4761 bp |
| Locus ID | 2736 |
| UniProt ID | P10070 |
| Protein Families | Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS |
| Protein Pathways | Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer |
| MW | 167.8 kDa |
| Gene Summary | This gene encodes a protein which belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (4) lacks an internal 51 nt, but maintains the same reading frame as variant 1 until the second exclusion. This exclusion occurs 1231 nt from the 3' end of the coding region and causes a frameshift. Thus, the resulting iso |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Vitamin D3 triggers antitumor activity through targeting hedgehog signaling in human renal cell carcinoma
,Valérian Dormoy, Claire Béraud, Véronique Lindner, Catherine Coquard, Mariette Barthelmebs, David Brasse, Didier Jacqmin, Hervé Lang, and Thierry Massfelder,
Carcinogenesis, Nov 2012; 33: 2084 - 2093.
[GLI2]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217291 | GLI2 (Myc-DDK-tagged)-Human GLI family zinc finger 2 (GLI2) |
CNY 16,280.00 |
|
| RC217291L1 | Lenti ORF clone of Human GLI family zinc finger 2 (GLI2), Myc-DDK-tagged |
CNY 18,680.00 |
|
| RC217291L2 | Lenti ORF clone of Human GLI family zinc finger 2 (GLI2), mGFP tagged |
CNY 18,680.00 |
|
| RC217291L3 | Lenti ORF clone of Human GLI family zinc finger 2 (GLI2), Myc-DDK-tagged |
CNY 15,680.00 |
|
| RC217291L4 | Lenti ORF clone of Human GLI family zinc finger 2 (GLI2), mGFP tagged |
CNY 18,680.00 |
|
| RG217291 | GLI2 (tGFP-tagged) - Human GLI family zinc finger 2 (GLI2) |
CNY 17,880.00 |
|
| SC303613 | GLI2 (untagged)-Human GLI family zinc finger 2 (GLI2) |
CNY 8,928.00 |
