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CLCN3 (NM_173872) Human Untagged Clone
CAT＃: SC318344
CLCN3 (untagged)-Human chloride channel 3 (CLCN3), transcript variant e
CNY 16,720.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ClC-3; CLC3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC318344 representing NM_173872.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTA CCGAGGAGATCTGCCGCCGCGATCGCCGGCGCGCCC ATGGAGTCTGAGCAGCTGTTCCATAGAGGCTACTATAGAAACAGCTACAACAGTATAACAAGTGCAAGT AGTGATGAGGAACTTTTAGATGGAGCAGGTGTTATTATGGACTTTCAAACATCTGAAGATGACAATTTA TTAGATGGTGACACTGCAGTTGGAACTCATTATACAATGACAAATGGAGGCAGCATTAACAGTTCTACA CATTTACTGGATCTTTTGGATGAACCAATTCCAGGTGTTGGTACATATGATGATTTCCATACTATTGAT TGGGTGCGAGAAAAATGTAAAGACAGAGAAAGGCATAGACGGATCAACAGCAAAAAGAAAGAATCAGCA TGGGAAATGACAAAAAGTTTGTATGATGCGTGGTCAGGATGGCTAGTAGTAACACTAACAGGATTGGCA TCAGGGGCACTGGCCGGATTAATAGACATTGCTGCCGATTGGATGACTGACCTAAAGGAGGGCATTTGC CTTAGTGCGTTGTGGTACAACCACGAACAGTGCTGTTGGGGATCTAATGAAACAACATTTGAAGAGAGG GATAAATGTCCACAGTGGAAAACATGGGCAGAATTAATCATAGGTCAAGCAGAGGGTCCTGGTTCTTAT ATCATGAACTACATAATGTACATCTTCTGGGCCTTGAGTTTTGCCTTTCTTGCAGTTTCCCTGGTAAAG GTATTTGCTCCATATGCCTGTGGCTCTGGAATTCCAGAGATTAAAACTATTTTAAGTGGATTCATCATC AGAGGTTACTTGGGAAAATGGACTTTAATGATTAAAACCATCACATTAGTCCTGGCTGTGGCATCAGGT TTGAGTTTAGGAAAAGAAGGTCCCCTGGTACATGTTGCCTGTTGCTGCGGAAATATCTTTTCCTACCTC TTTCCAAAGTATAGCACAAACGAAGCTAAAAAAAGGGAGGTGCTATCAGCTGCCTCAGCTGCAGGGGTT TCTGTAGCTTTTGGTGCACCAATTGGAGGAGTTCTTTTTAGCCTGGAAGAGGTTAGCTATTATTTTCCT CTCAAAACTTTATGGAGATCATTTTTTGCTGCTTTAGTGGCTGCATTTGTTTTGAGGTCCATCAATCCA TTTGGTAACAGCCGTCTGGTCCTTTTTTATGTGGAGTATCATACACCATGGTACCTTTTTGAACTGTTT CCTTTTATTCTTCTAGGGGTATTTGGAGGGCTTTGGGGAGCCTTTTTCATTAGGGCAAATATTGCCTGG TGTCGTCGACGCAAGTCCACGAAATTTGGAAAGTATCCCGTTCTGGAAGTCATTATTGTTGCAGCCATT ACTGCTGTGATAGCCTTCCCTAATCCATACACTAGGCTAAACACCAGTGAACTGATCAAAGAGCTTTTT ACAGACTGTGGTCCCCTGGAATCCTCTTCTCTTTGTGACTACAGAAATGACATGAATGCCAGTAAAATT GTCGATGACATTCCTGATCGTCCAGCAGGCATTGGAGTATATTCAGCTATATGGCAGTTATGCCTGGCA CTCATATTTAAAATCATAATGACAGTATTCACTTTTGGCATCAAGGTTCCATCAGGCTTGTTCATCCCC AGCATGGCCATTGGAGCGATCGCAGGAAGGATTGTGGGGATTGCGGTGGAGCAGCTTGCCTACTATCAC CACGACTGGTTTATCTTTAAGGAGTGGTGTGAGGTCGGGGCTGATTGCATTACACCTGGCCTTTATGCC ATGGTTGGTGCTGCTGCATGCTTAGGTGGTGTGACAAGAATGACTGTCTCCCTGGTGGTTATTGTTTTT GAGCTTACTGGAGGCTTGGAATATATTGTTCCCCTTATGGCTGCAGTCATGACCAGTAAATGGGTTGGA GATGCCTTTGGCAGGGAAGGCATTTATGAAGCACACATCCGATTAAATGGATACCCTTTCTTGGATGCA AAAGAAGAATTCACTCATACCACCCTGGCTGCTGACGTTATGAGACCTCGAAGGAATGATCCTCCCTTA GCTGTCCTGACACAGGACAATATGACAGTGGATGATATAGAAAACATGATTAATGAAACCAGCTACAAT GGATTTCCTGTCATAATGTCAAAAGAATCTCAGAGATTAGTGGGATTTGCCCTCAGAAGAGACCTGACA ATTGCAATAGAAAGTGCCAGGAAAAAACAAGAAGGTATCGTTGGCAGTTCTCGGGTGTGTTTTGCACAG CACACCCCATCTCTTCCAGCAGAAAGTCCTCGGCCATTGAAGCTTCGAAGCATTCTTGACATGAGCCCT TTTACAGTGACAGACCACACCCCAATGGAGATCGTGGTGGATATTTTCCGAAAGCTGGGACTGAGGCAG TGCCTTGTAACTCACAATGGGATTGTCTTGGGGATCATCACAAAGAAGAACATATTAGAGCATCTCGAG CAACTAAAGCAGCACGTCGAACCCTTGGCGCCTCCTTGGCATTATAACAAAAAAAGATATCCTCCGGCA TATGGCCCAGACGGCAAACCAAGACCCCGCTTCAATAATGTTCAACTGAATCTCACAGATGAGGAGAGA GAAGAAACGGAAGAGGAAGTTTATTTGTTGAATAGCACAACTCTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | AscI-MluI |
| ACCN | NM_173872 |
| Insert Size | 2601 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_173872.3 |
| RefSeq Size | 6299 bp |
| RefSeq ORF | 2601 bp |
| Locus ID | 1182 |
| UniProt ID | P51790 |
| Protein Families | Druggable Genome, Ion Channels: Other, Transmembrane |
| MW | 96.7 kDa |
| Gene Summary | This gene encodes a member of the voltage-gated chloride channel (ClC) family. The encoded protein is present in all cell types and localized in plasma membranes and in intracellular vesicles. It is a multi-pass membrane protein which contains a ClC domain and two additional C-terminal CBS (cystathionine beta-synthase) domains. The ClC domain catalyzes the selective flow of Cl- ions across cell membranes, and the CBS domain may have a regulatory function. This protein plays a role in both acidification and transmitter loading of GABAergic synaptic vesicles, and in smooth muscle cell activation and neointima formation. This protein is required for lysophosphatidic acid (LPA)-activated Cl- current activity and fibroblast-to-myofibroblast differentiation. The protein activity is regulated by Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) in glioma cells. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011] Transcript Variant: This variant (e) has an additional exon in the 3' coding region, which results in frame-shift, compared to variant b. The resulting isoform (e) has a longer and distinct C-terminus, compared to isoform b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214183 | CLCN3 (Myc-DDK-tagged)-Human chloride channel 3 (CLCN3), transcript variant e |
CNY 6,344.00 |
|
| RC214183L3 | Lenti-ORF clone of CLCN3 (Myc-DDK-tagged)-Human chloride channel 3 (CLCN3), transcript variant e |
CNY 11,120.00 |
|
| RC214183L4 | Lenti-ORF clone of CLCN3 (mGFP-tagged)-Human chloride channel 3 (CLCN3), transcript variant e |
CNY 11,120.00 |
|
| RG214183 | CLCN3 (tGFP-tagged) - Human chloride channel 3 (CLCN3), transcript variant e |
CNY 7,944.00 |
|
| SC310074 | CLCN3 (untagged)-Human chloride channel 3 (CLCN3), transcript variant e |
CNY 16,720.00 |
