VILL (NM_015873) Human Untagged Clone

CAT#: SC318333

VILL (untagged)-Human villin-like (VILL)



  "NM_015873" in other vectors (4)

CNY 16,530.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


VILL rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC318333 representing NM_015873.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGACATCAGCAAGGGCCTCCCAGGCATGCAGGGAGGCCTCCACATATGGATCTCTGAGAACCGGAAG
ATGGTGCCGGTACCCGAGGGGGCTTACGGGAACTTTTTTGAGGAACACTGCTATGTCATCCTCCACGTC
CCCCAGAGCCCGAAGGCCACGCAGGGGGCGTCCAGCGACCTGCACTACTGGGTCGGGAAGCAGGCGGGT
GCGGAAGCGCAGGGCGCTGCGGAGGCCTTCCAGCAGCGCCTACAGGACGAGCTGGGGGGCCAGACCGTG
CTGCACCGCGAGGCGCAGGGCCACGAGTCCGACTGCTTCTGCAGCTACTTCCGCCCGGGAATCATCTAC
AGGAAGGGAGGCCTAGCATCTGACCTCAAGCATGTGGAGACCAACTTGTTCAACATCCAGCGACTGCTG
CACATCAAAGGGAGGAAGCACGTGTCTGCCACTGAGGTGGAGCTCTCCTGGAACAGCTTTAATAAGGGT
GACATCTTCCTGCTGGACCTAGGCAAGATGATGATTCAGTGGAATGGGCCCAAGACCAGCATTTCTGAG
AAGGCTCGGGGGCTGGCTTTGACCTACAGCCTCCGGGACAGGGAACGTGGTGGTGGTCGTGCACAGATT
GGTGTGGTGGATGATGAGGCCAAAGCCCCGGACCTCATGCAGATCATGGAGGCTGTGCTGGGCCGCAGG
GTGGGCAGCCTGCGTGCCGCCACGCCCAGCAAGGATATCAACCAGCTGCAGAAGGCCAATGTTCGCCTG
TACCATGTCTATGAGAAGGGCAAAGACCTGGTGGTCCTGGAGTTGGCGACCCCCCCACTGACCCAGGAC
CTGCTGCAGGAGGAGGACTTCTACATCCTGGACCAGGGTGGCTTCAAGATCTATGTGTGGCAGGGACGC
ATGTCTAGCCTCCAGGAGAGAAAGGCTGCCTTCAGCCGGGCTGTGGGCTTCATCCAGGCCAAGGGCTAC
CCGACCTACACCAACGTGGAGGTGGTGAACGACGGCGCCGAGTCGGCCGCGTTCAAGCAGCTCTTCCGG
ACTTGGTCTGAGAAGCGGCGCAGGAACCAGAAGCTCGGCGGGAGGGATAAATCGATTCATGTAAAGCTG
GACGTGGGCAAGCTGCACACCCAGCCTAAGTTAGCGGCCCAGCTCAGGATGGTGGACGACGGCTCTGGG
AAGGTGGAGGTGTGGTGCATCCAGGACTTACACAGGCAGCCCGTGGACCCCAAGCGTCATGGACAGCTG
TGTGCAGGCAACTGCTACCTTGTGCTCTACACATACCAGAGGCTGGGCCGTGTCCAGTACATCCTGTAC
CTATGGCAGGGCCACCAGGCCACTGCGGATGAGATTGAGGCCCTGAACAGCAACGCTGAGGAACTAGAT
GTCATGTATGGTGGCGTCCTAGTACAGGAGCATGTGACCATGGGCAGCGAGCCCCCCCACTTCCTCGCC
ATCTTCCAGGGCCAGCTGGTGATCTTCCAGGAGAGAGCTGGGCACCATGGAAAGGGGCAGTCAGCATCC
ACCACAAGGCTTTTCCAAGTGCAAGGCACTGACAGCCACAACACCAGGACCATGGAGGTGCCAGCCCGT
GCCTCATCCCTCAACTCCAGTGACATCTTCTTGCTGGTCACAGCCAGCGTCTGCTACCTCTGGTTTGGG
AAGGGCTGTAATGGTGATCAGCGTGAGATGGCACGGGTGGTGGTCACTGTCATTTCCAGGAAGAATGAG
GAAACGGTGCTGGAGGGTCAGGAGCCTCCCCACTTCTGGGAGGCCCTGGGAGGCCGGGCCCCCTACCCC
AGCAACAAGAGGCTCCCTGAGGAGGTCCCCAGCTTCCAGCCACGACTGTTTGAGTGCTCCAGCCACATG
GGCTGCCTGGTCCTCGCAGAAGTGGGGTTCTTCAGCCAGGAGGACCTGGACAAGTATGACATCATGTTA
CTGGACACCTGGCAGGAGATCTTCCTGTGGCTTGGGGAAGCTGCAAGTGAGTGGAAGGAGGCGGTGGCC
TGGGGCCAGGAGTACCTGAAGACTCACCCAGCAGGGAGGAGCCCGGCCACACCCATCGTGCTGGTCAAG
CAGGGCCATGAGCCTCCCACCTTCATTGGATGGTTCTTCACTTGGGACCCCTACAAGTGGACTAGCCAC
CCGTCCCACAAGGAAGTGGTGGATGGCAGCCCGGCAGCAGCATCAACCATCTCTGAGATAACAGCAGAA
GTCAACAACTTGCGGCTATCCAGATGGCCGGGCAATGGCAGGGCAGGTGCCGTGGCCCTGCAGGCCCTC
AAGGGCTCCCAGGACAGCTCAGAGAATGATCTGGTGCGAAGCCCCAAGTCGGCTGGCAGCAGAACCAGC
AGCTCCGTCAGCAGCACCAGCGCCACGATCAACGGGGGCCTGCGCCGGGAACAACTGATGCACCAGGCT
GTTGAGGACCTGCCAGAGGGCGTGGACCCTGCCCGCAGGGAGTTCTATCTCTCAGACTCTGACTTCCAA
GATATCTTTGGGAAATCCAAGGAGGAATTCTACAGCATGGCCACGTGGAGGCAGCGGCAGGAGAAAAAG
CAGCTGGGCTTCTTCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_015873
Insert Size 2571 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_015873.3
RefSeq Size 2787 bp
RefSeq ORF 2571 bp
Locus ID 50853
UniProt ID O15195
Domains VHP, GEL, Gelsolin
MW 95.9 kDa
Gene Summary The protein encoded by this gene belongs to the villin/gelsolin family. It contains 6 gelsolin-like repeats and a headpiece domain. It may play a role in actin-bundling. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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