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VILL (NM_015873) Human Tagged ORF Clone
CAT＃: RG215217
- TrueORF®
VILL (tGFP-tagged) - Human villin-like (VILL)
ORF Plasmid: DDK
"NM_015873" in other vectors (4)
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CNY 7,890.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG215217 representing NM_015873
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACATCAGCAAGGGCCTCCCAGGCATGCAGGGAGGCCTCCACATATGGATCTCTGAGAACCGGAAGA TGGTGCCGGTACCCGAGGGGGCTTACGGGAACTTTTTTGAGGAACACTGCTATGTCATCCTCCACGTCCC CCAGAGCCCGAAGGCCACGCAGGGGGCGTCCAGCGACCTGCACTACTGGGTCGGGAAGCAGGCGGGTGCG GAAGCGCAGGGCGCTGCGGAGGCCTTCCAGCAGCGCCTACAGGACGAGCTGGGGGGCCAGACCGTGCTGC ACCGCGAGGCGCAGGGCCACGAGTCCGACTGCTTCTGCAGCTACTTCCGCCCGGGAATCATCTACAGGAA GGGAGGCCTAGCATCTGACCTCAAGCATGTGGAGACCAACTTGTTCAACATCCAGCGACTGCTGCACATC AAAGGGAGGAAGCACGTGTCTGCCACTGAGGTGGAGCTCTCCTGGAACAGCTTTAATAAGGGTGACATCT TCCTGCTGGACCTAGGCAAGATGATGATTCAGTGGAATGGGCCCAAGACCAGCATTTCTGAGAAGGCTCG GGGGCTGGCTTTGACCTACAGCCTCCGGGACAGGGAACGTGGTGGTGGTCGTGCACAGATTGGTGTGGTG GATGATGAGGCCAAAGCCCCGGACCTCATGCAGATCATGGAGGCTGTGCTGGGCCGCAGGGTGGGCAGCC TGCGTGCCGCCACGCCCAGCAAGGATATCAACCAGCTGCAGAAGGCCAATGTTCGCCTGTACCATGTCTA TGAGAAGGGCAAAGACCTGGTGGTCCTGGAGTTGGCGACCCCCCCACTGACCCAGGACCTGCTGCAGGAG GAGGACTTCTACATCCTGGACCAGGGTGGCTTCAAGATCTATGTGTGGCAGGGACGCATGTCTAGCCTCC AGGAGAGAAAGGCTGCCTTCAGCCGGGCTGTGGGCTTCATCCAGGCCAAGGGCTACCCGACCTACACCAA CGTGGAGGTGGTGAACGACGGCGCCGAGTCGGCCGCGTTCAAGCAGCTCTTCCGGACTTGGTCTGAGAAG CGGCGCAGGAACCAGAAGCTCGGCGGGAGGGATAAATCGATTCATGTAAAGCTGGACGTGGGCAAGCTGC ACACCCAGCCTAAGTTAGCGGCCCAGCTCAGGATGGTGGACGACGGCTCTGGGAAGGTGGAGGTGTGGTG CATCCAGGACTTACACAGGCAGCCCGTGGACCCCAAGCGTCATGGACAGCTGTGTGCAGGCAACTGCTAC CTTGTGCTCTACACATACCAGAGGCTGGGCCGTGTCCAGTACATCCTGTACCTATGGCAGGGCCACCAGG CCACTGCGGATGAGATTGAGGCCCTGAACAGCAACGCTGAGGAACTAGATGTCATGTATGGTGGCGTCCT AGTACAGGAGCATGTGACCATGGGCAGCGAGCCCCCCCACTTCCTCGCCATCTTCCAGGGCCAGCTGGTG ATCTTCCAGGAGAGAGCTGGGCACCATGGAAAGGGGCAGTCAGCATCCACCACAAGGCTTTTCCAAGTGC AAGGCACTGACAGCCACAACACCAGGACCATGGAGGTGCCAGCCCGTGCCTCATCCCTCAACTCCAGTGA CATCTTCTTGCTGGTCACAGCCAGCGTCTGCTACCTCTGGTTTGGGAAGGGCTGTAATGGTGATCAGCGT GAGATGGCACGGGTGGTGGTCACTGTCATTTCCAGGAAGAATGAGGAAACGGTGCTGGAGGGTCAGGAGC CTCCCCACTTCTGGGAGGCCCTGGGAGGCCGGGCCCCCTACCCCAGCAACAAGAGGCTCCCTGAGGAGGT CCCCAGCTTCCAGCCACGACTGTTTGAGTGCTCCAGCCACATGGGCTGCCTGGTCCTCGCAGAAGTGGGG TTCTTCAGCCAGGAGGACCTGGACAAGTATGACATCATGTTACTGGACACCTGGCAGGAGATCTTCCTGT GGCTTGGGGAAGCTGCAAGTGAGTGGAAGGAGGCGGTGGCCTGGGGCCAGGAGTACCTGAAGACTCACCC AGCAGGGAGGAGCCCGGCCACACCCATCGTGCTGGTCAAGCAGGGCCATGAGCCTCCCACCTTCATTGGA TGGTTCTTCACTTGGGACCCCTACAAGTGGACTAGCCACCCGTCCCACAAGGAAGTGGTGGATGGCAGCC CGGCAGCAGCATCAACCATCTCTGAGATAACAGCAGAAGTCAACAACTTGCGGCTATCCAGATGGCCGGG CAATGGCAGGGCAGGTGCCGTGGCCCTGCAGGCCCTCAAGGGCTCCCAGGACAGCTCAGAGAATGATCTG GTGCGAAGCCCCAAGTCGGCTGGCAGCAGAACCAGCAGCTCCGTCAGCAGCACCAGCGCCACGATCAACG GGGGCCTGCGCCGGGAACAACTGATGCACCAGGCTGTTGAGGACCTGCCAGAGGGCGTGGACCCTGCCCG CAGGGAGTTCTATCTCTCAGACTCTGACTTCCAAGATATCTTTGGGAAATCCAAGGAGGAATTCTACAGC ATGGCCACGTGGAGGCAGCGGCAGGAGAAAAAGCAGCTGGGCTTCTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG215217 representing NM_015873
Red=Cloning site Green=Tags(s) MDISKGLPGMQGGLHIWISENRKMVPVPEGAYGNFFEEHCYVILHVPQSPKATQGASSDLHYWVGKQAGA EAQGAAEAFQQRLQDELGGQTVLHREAQGHESDCFCSYFRPGIIYRKGGLASDLKHVETNLFNIQRLLHI KGRKHVSATEVELSWNSFNKGDIFLLDLGKMMIQWNGPKTSISEKARGLALTYSLRDRERGGGRAQIGVV DDEAKAPDLMQIMEAVLGRRVGSLRAATPSKDINQLQKANVRLYHVYEKGKDLVVLELATPPLTQDLLQE EDFYILDQGGFKIYVWQGRMSSLQERKAAFSRAVGFIQAKGYPTYTNVEVVNDGAESAAFKQLFRTWSEK RRRNQKLGGRDKSIHVKLDVGKLHTQPKLAAQLRMVDDGSGKVEVWCIQDLHRQPVDPKRHGQLCAGNCY LVLYTYQRLGRVQYILYLWQGHQATADEIEALNSNAEELDVMYGGVLVQEHVTMGSEPPHFLAIFQGQLV IFQERAGHHGKGQSASTTRLFQVQGTDSHNTRTMEVPARASSLNSSDIFLLVTASVCYLWFGKGCNGDQR EMARVVVTVISRKNEETVLEGQEPPHFWEALGGRAPYPSNKRLPEEVPSFQPRLFECSSHMGCLVLAEVG FFSQEDLDKYDIMLLDTWQEIFLWLGEAASEWKEAVAWGQEYLKTHPAGRSPATPIVLVKQGHEPPTFIG WFFTWDPYKWTSHPSHKEVVDGSPAAASTISEITAEVNNLRLSRWPGNGRAGAVALQALKGSQDSSENDL VRSPKSAGSRTSSSVSSTSATINGGLRREQLMHQAVEDLPEGVDPARREFYLSDSDFQDIFGKSKEEFYS MATWRQRQEKKQLGFF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015873 |
| ORF Size | 2568 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015873.3, NP_056957.3 |
| RefSeq Size | 2787 bp |
| RefSeq ORF | 2571 bp |
| Locus ID | 50853 |
| UniProt ID | O15195 |
| Domains | VHP, GEL, Gelsolin |
| Gene Summary | The protein encoded by this gene belongs to the villin/gelsolin family. It contains 6 gelsolin-like repeats and a headpiece domain. It may play a role in actin-bundling. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215217 | VILL (Myc-DDK-tagged)-Human villin-like (VILL) |
CNY 6,272.00 |
|
| RC215217L3 | Lenti ORF clone of Human villin-like (VILL), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC215217L4 | Lenti ORF clone of Human villin-like (VILL), mGFP tagged |
CNY 9,030.00 |
|
| SC318333 | VILL (untagged)-Human villin-like (VILL) |
CNY 16,530.00 |
