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RAPGEF5 (NM_012294) Human Untagged Clone
CAT＃: SC318214
RAPGEF5 (untagged)-Human Rap guanine nucleotide exchange factor (GEF) 5 (RAPGEF5)
CNY 14,060.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GFR; MR-GEF; REPAC |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC318214 representing NM_012294.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCATAGGAGTCTGGCAACTCCTACTGGACATGGGAATTATGTTATCAGTGGACCAGCATCTATAC TTTCAAGATACTTATGTTTTCTACCAGTTTTCCTCTGATGAATGTAGCTACTTGTACTGTGAATTTGAA AGAGAAGAAGAATGGCAAAATGGTGTCAAGCTTTTACTGCAACTTGTGCCTCTCATTCCTGCCAGAGGT GGCATCTGTGAACTGTCTCATCAGAAAATTGAAGACTCCGAAGAAAGCAGTGATGAAATTCTTGTGCGT CTAACATCTGCGGTGCAGAGAGAGCTAGCAGCTGTTATTGCTTTGAAAGCAAGGAAGTCTGCAATTGAA CAAGATGAAGAAAACAACGACAAACATGTAGCTGTAACAGAAGCCGAAAGTGTTCCAGATTCTCAGGCA GGGGTGATGTGCAAGCTCCAGGAAAGAGATGAAATCGGACGAATTGAACTAGTCCAGAAGCTGGCAAAA GAAAACTATCAGTTTTTGCAGACGGACAAAAAAGAACAGGAGAAGTCTGAACACCAAGATGATGAAGTG ACGACTGTTCAGGTTAAAGAGCAAGACCAGAGCGTCCTGGTGCTGAAGAAAGTGCAGTGCTGTGGCCCA GCCCCCACAGCTGGGAGTGCGGAGAGCCATTGGAGATATGTGGTGGTGTCCGGGACCCCGGAGAAGATT TTGGAGCACCTTTTGAATGACTTGCACCTGGAAGAAGTCCAGGACAAAGAAACAGAGACCCTCCTGGAT GACTTCCTTCTCACGTACACTGTCTTCATGACAACTGATGACTTGTGCCAGGCTCTGTTAAGGCACTAT TCTGCTAAGAAGTATCAAGGCAAAGAGGAAAACTCAGACGTTCCGCGTAGGAAACGTAAAGTCTTGCAT CTTGTTTCCCAGTGGATTGCTCTGTACAAAGACTGGTTACCTGAAGATGAACATTCAAAAATGTTTTTA AAGACCATATATAGGAATGTACTGGATGATGTTTATGAATATCCAATACTTGAAAAAGAATTGAAAGAA TTTCAAAAGATACTTGGAATGCACCGTCGTCACACTGTAGATGAATATTCACCACAAAAAAAGAATAAA GCCCTTTTCCACCAATTCAGTCTTAAGGAGAACTGGCTCCAGCATAGAGGAACTGTGACTGAAACGGAG GAAATTTTCTGCCACGTGTATATAACAGAGCACTCCTATGTCAGTGTGAAGGCAAAAGTTTCCAGTATA GCCCAAGAGATCCTAAAAGTCGTGGCAGAAAAGATCCAGTATGCAGAAGAGGATCTGGCTCTGGTGGCC ATCACATTCTCTGGGGAAAAGCATGAACTTCAGCCAAATGACTTAGTCATCTCCAAATCCCTCGAGGCA TCTGGTCGAATATATGTCTACCGGAAAGACCTGGCGGACACTTTGAACCCATTTGCAGAAAATGAGGAA TCACAGCAAAGGTCGATGAGGATTTTGGGAATGAACACTTGGGATCTTGCTCTGGAATTAATGAATTTT GATTGGAGTCTATTCAATTCAATTCACGAGCAAGAGCTGATCTACTTCACGTTCAGCAGACAGGGAAGT GGGGAACACACTGCAAATCTCAGCCTTCTGCTCCAGAGATGCAATGAGGTCCAGCTTTGGGTGGCCACG GAGATTCTGCTCTGCAGCCAGCTGGGCAAGCGAGTGCAGCTGGTGAAAAAATTCATCAAAATTGCGGCT CACTGCAAAGCCCAGAGAAACCTGAATTCTTTCTTTGCCATTGTGATGGGTCTCAACACTGCTTCTGTC AGTCGACTGTCGCAGACCTGGGAGAAAATCCCTGGGAAGTTTAAGAAACTTTTCTCTGAACTTGAAAGT TTAACAGATCCTTCCCTAAATCACAAAGCCTACAGAGATGCATTCAAAAAGATGAAGCCACCAAAAATC CCTTTCATGCCCTTATTGCTTAAAGATGTAACATTTATTCATGAAGGAAATAAAACTTTTTTGGATAAT CTTGTCAATTTTGAAAAGCTGCATATGATCGCAGACACTGTCCGAACCCTGAGACACTGCAGGACTAAC CAGTTTGGTGACCTGTCTCCAAAAGAGCATCAAGAGTTAAAGTCCTATGTTAATCACCTGTATGTCATT GACAGCCAGCAGGCTCTGTTTGAGCTCTCACACAGGATCGAGCCTCGGGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_012294 |
| Insert Size | 2193 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012294.3 |
| RefSeq Size | 6629 bp |
| RefSeq ORF | 2193 bp |
| Locus ID | 9771 |
| UniProt ID | Q92565 |
| MW | 84.7 kDa |
| Gene Summary | Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF5, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. CCDS Note: The coding region has been updated to extend the N-terminus to one that is more supported by available conservation data. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218010 | RAPGEF5 (Myc-DDK-tagged)-Human Rap guanine nucleotide exchange factor (GEF) 5 (RAPGEF5) |
CNY 5,344.00 |
|
| RC218010L3 | Lenti ORF clone of Human Rap guanine nucleotide exchange factor (GEF) 5 (RAPGEF5), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC218010L4 | Lenti ORF clone of Human Rap guanine nucleotide exchange factor (GEF) 5 (RAPGEF5), mGFP tagged |
CNY 7,700.00 |
|
| RG218010 | RAPGEF5 (tGFP-tagged) - Human Rap guanine nucleotide exchange factor (GEF) 5 (RAPGEF5) |
CNY 6,370.00 |
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