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RAPGEF5 (NM_012294) Human Tagged ORF Clone
CAT＃: RC218010
RAPGEF5 (Myc-DDK-tagged)-Human Rap guanine nucleotide exchange factor (GEF) 5 (RAPGEF5)
ORF Plasmid: tGFP
"NM_012294" in other vectors (4)
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CNY 5,344.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GFR; MR-GEF; REPAC |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC218010 representing NM_012294
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCATAGGAGTCTGGCAACTCCTACTGGACATGGGAATTATGTTATCAGTGGACCAGCATCTATACT TTCAAGATACTTATGTTTTCTACCAGTTTTCCTCTGATGAATGTAGCTACTTGTACTGTGAATTTGAAAG AGAAGAAGAATGGCAAAATGGTGTCAAGCTTTTACTGCAACTTGTGCCTCTCATTCCTGCCAGAGGTGGC ATCTGTGAACTGTCTCATCAGAAAATTGAAGACTCCGAAGAAAGCAGTGATGAAATTCTTGTGCGTCTAA CATCTGCGGTGCAGAGAGAGCTAGCAGCTGTTATTGCTTTGAAAGCAAGGAAGTCTGCAATTGAACAAGA TGAAGAAAACAACGACAAACATGTAGCTGTAACAGAAGCCGAAAGTGTTCCAGATTCTCAGGCAGGGGTG ATGTGCAAGCTCCAGGAAAGAGATGAAATCGGACGAATTGAACTAGTCCAGAAGCTGGCAAAAGAAAACT ATCAGTTTTTGCAGACGGACAAAAAAGAACAGGAGAAGTCTGAACACCAAGATGATGAAGTGACGACTGT TCAGGTTAAAGAGCAAGACCAGAGCGTCCTGGTGCTGAAGAAAGTGCAGTGCTGTGGCCCAGCCCCCACA GCTGGGAGTGCGGAGAGCCATTGGAGATATGTGGTGGTGTCCGGGACCCCGGAGAAGATTTTGGAGCACC TTTTGAATGACTTGCACCTGGAAGAAGTCCAGGACAAAGAAACAGAGACCCTCCTGGATGACTTCCTTCT CACGTACACTGTCTTCATGACAACTGATGACTTGTGCCAGGCTCTGTTAAGGCACTATTCTGCTAAGAAG TATCAAGGCAAAGAGGAAAACTCAGACGTTCCGCGTAGGAAACGTAAAGTCTTGCATCTTGTTTCCCAGT GGATTGCTCTGTACAAAGACTGGTTACCTGAAGATGAACATTCAAAAATGTTTTTAAAGACCATATATAG GAATGTACTGGATGATGTTTATGAATATCCAATACTTGAAAAAGAATTGAAAGAATTTCAAAAGATACTT GGAATGCACCGTCGTCACACTGTAGATGAATATTCACCACAAAAAAAGAATAAAGCCCTTTTCCACCAAT TCAGTCTTAAGGAGAACTGGCTCCAGCATAGAGGAACTGTGACTGAAACGGAGGAAATTTTCTGCCACGT GTATATAACAGAGCACTCCTATGTCAGTGTGAAGGCAAAAGTTTCCAGTATAGCCCAAGAGATCCTAAAA GTCGTGGCAGAAAAGATCCAGTATGCAGAAGAGGATCTGGCTCTGGTGGCCATCACATTCTCTGGGGAAA AGCATGAACTTCAGCCAAATGACTTAGTCATCTCCAAATCCCTCGAGGCATCTGGTCGAATATATGTCTA CCGGAAAGACCTGGCGGACACTTTGAACCCATTTGCAGAAAATGAGGAATCACAGCAAAGGTCGATGAGG ATTTTGGGAATGAACACTTGGGATCTTGCTCTGGAATTAATGAATTTTGATTGGAGTCTATTCAATTCAA TTCACGAGCAAGAGCTGATCTACTTCACGTTCAGCAGACAGGGAAGTGGGGAACACACTGCAAATCTCAG CCTTCTGCTCCAGAGATGCAATGAGGTCCAGCTTTGGGTGGCCACGGAGATTCTGCTCTGCAGCCAGCTG GGCAAGCGAGTGCAGCTGGTGAAAAAATTCATCAAAATTGCGGCTCACTGCAAAGCCCAGAGAAACCTGA ATTCTTTCTTTGCCATTGTGATGGGTCTCAACACTGCTTCTGTCAGTCGACTGTCGCAGACCTGGGAGAA AATCCCTGGGAAGTTTAAGAAACTTTTCTCTGAACTTGAAAGTTTAACAGATCCTTCCCTAAATCACAAA GCCTACAGAGATGCATTCAAAAAGATGAAGCCACCAAAAATCCCTTTCATGCCCTTATTGCTTAAAGATG TAACATTTATTCATGAAGGAAATAAAACTTTTTTGGATAATCTTGTCAATTTTGAAAAGCTGCATATGAT CGCAGACACTGTCCGAACCCTGAGACACTGCAGGACTAACCAGTTTGGTGACCTGTCTCCAAAAGAGCAT CAAGAGTTAAAGTCCTATGTTAATCACCTGTATGTCATTGACAGCCAGCAGGCTCTGTTTGAGCTCTCAC ACAGGATCGAGCCTCGGGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC218010 representing NM_012294
Red=Cloning site Green=Tags(s) MAIGVWQLLLDMGIMLSVDQHLYFQDTYVFYQFSSDECSYLYCEFEREEEWQNGVKLLLQLVPLIPARGG ICELSHQKIEDSEESSDEILVRLTSAVQRELAAVIALKARKSAIEQDEENNDKHVAVTEAESVPDSQAGV MCKLQERDEIGRIELVQKLAKENYQFLQTDKKEQEKSEHQDDEVTTVQVKEQDQSVLVLKKVQCCGPAPT AGSAESHWRYVVVSGTPEKILEHLLNDLHLEEVQDKETETLLDDFLLTYTVFMTTDDLCQALLRHYSAKK YQGKEENSDVPRRKRKVLHLVSQWIALYKDWLPEDEHSKMFLKTIYRNVLDDVYEYPILEKELKEFQKIL GMHRRHTVDEYSPQKKNKALFHQFSLKENWLQHRGTVTETEEIFCHVYITEHSYVSVKAKVSSIAQEILK VVAEKIQYAEEDLALVAITFSGEKHELQPNDLVISKSLEASGRIYVYRKDLADTLNPFAENEESQQRSMR ILGMNTWDLALELMNFDWSLFNSIHEQELIYFTFSRQGSGEHTANLSLLLQRCNEVQLWVATEILLCSQL GKRVQLVKKFIKIAAHCKAQRNLNSFFAIVMGLNTASVSRLSQTWEKIPGKFKKLFSELESLTDPSLNHK AYRDAFKKMKPPKIPFMPLLLKDVTFIHEGNKTFLDNLVNFEKLHMIADTVRTLRHCRTNQFGDLSPKEH QELKSYVNHLYVIDSQQALFELSHRIEPRV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012294 |
| ORF Size | 2190 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012294.3, NP_036426.3 |
| RefSeq Size | 6629 bp |
| RefSeq ORF | 2652 bp |
| Locus ID | 9771 |
| UniProt ID | Q92565 |
| MW | 84.5 kDa |
| Gene Summary | Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF5, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218010L3 | Lenti ORF clone of Human Rap guanine nucleotide exchange factor (GEF) 5 (RAPGEF5), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC218010L4 | Lenti ORF clone of Human Rap guanine nucleotide exchange factor (GEF) 5 (RAPGEF5), mGFP tagged |
CNY 7,700.00 |
|
| RG218010 | RAPGEF5 (tGFP-tagged) - Human Rap guanine nucleotide exchange factor (GEF) 5 (RAPGEF5) |
CNY 6,370.00 |
|
| SC318214 | RAPGEF5 (untagged)-Human Rap guanine nucleotide exchange factor (GEF) 5 (RAPGEF5) |
CNY 14,060.00 |
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