United States
Germany
Japan
United Kingdom
China
ZCWPW1 (NM_017984) Human Untagged Clone
CAT＃: SC318109
ZCWPW1 (untagged)-Human zinc finger, CW type with PWWP domain 1 (ZCWPW1)
CN¥ 10,360.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ZCW1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC318109 representing NM_017984.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGATGACAACGTTGCAGAATAAAGAAGAATGTGGAAAGGGACCAAAGAGAATCTTTGCCCCACCTGCA CAAAAATCTTACAGCCTGTTACCTTGTAGCCCTAACTCCCCTAAGGAGGAGACCCCGGGGATCAGTTCC CCAGAGACAGAGGCCAGGATAAGCCTGCCAAAGGCCAGTTTAAAGAAGAAAGAGGAAAAAGCAACCATG AAGAATGTTCCAAGCAGGGAACAGGAGAAAAAAAGAAAGGCACAAATCAACAAGCAAGCAGAGAAGAAA GAAAAGGAAAAATCAAGTCTTACCAATGCAGAATTTGAGGAGATTGTCCAGATTGTTCTGCAGAAGTCC CTTCAGGAGTGCTTGGGGATGGGATCTGGCCTTGATTTTGCAGAGACTTCTTGTGCCCAGCCCGTAGTA TCTACCCAATCAGACAAGGAGCCAGGAATTACTGCTTCTGCTACTGATACTGATAATGCTAATGGAGAG GAGGTACCACATACTCAAGAGATTTCAGTGTCTTGGGAAGGTGAAGCTGCCCCTGAGATAAGGACATCT AAGTTAGGCCAGCCAGATCCTGCACCCTCTAAGAAGAAATCCAATAGACTCACCTTAAGCAAAAGAAAG AAGGAAGCTCATGAGAAGGTGGAGAAAACTCAAGGTGGACATGAGCACAGACAGGAAGACCGACTAAAG AAAACAGTTCAGGATCATTCTCAGATCAGGGACCAGCAAAAAGGAGAGATAAGTGGTTTTGGTCAATGT CTGGTCTGGGTCCAGTGTTCCTTCCCAAACTGTGGGAAATGGAGGCGGCTGTGTGGGAACATTGACCCC TCAGTTCTCCCAGATAATTGGTCCTGTGATCAGAACACAGATGTGCAGTATAATCGCTGTGATATTCCT GAGGAGACCTGGACAGGGCTTGAGAGTGATGTGGCCTATGCCTCCTACATCCCAGGATCCATCATCTGG GCCAAGCAATACGGTTACCCCTGGTGGCCAGGCATGATAGAATCTGATCCTGACTTAGGGGAATATTTT CTTTTTACTTCCCATCTTGATTCCCTGCCGTCTAAGTACCATGTGACGTTTTTTGGAGAAACAGTTTCT CGTGCATGGATCCCAGTCAACATGCTAAAGAACTTCCAGGAGCTGTCCCTGGAGCTATCAGTCATGAAA AAGCGCAGAAATGACTGCAGCCAGAAACTGGGGGTGGCCCTGATGATGGCTCAAGAGGCAGAACAGATC AGCATTCAGGAACGGGTTAACTTGTTTGGTTTCTGGAGCCGATTCAACGGATCTAACAGTAATGGGGAA AGAAAAGACTTACAGCTCTCTGGTTTGAACAGCCCAGGATCCTGCTTAGAGAAAAAGGAGAAAGAGGAA GAGTTGGAAAAGGAGGAAGGAGAGAAAACAGACCCAATTTTGCCCATTCGTAAGCGAGTCAAAATACAG ACCCAAAAAACCAAGCCAAGAGGGCTTGGGGGTGATGCAGGCACAGCAGATGGCCGAGGCAGGACACTG CAGAGGAAGATAATGAAGAGATCTCTAGGCAGGAAATCCACAGCTCCTCCTGCACCCAGAATGGGAAGG AAAGAAGGCCAAGGGAATTCAGATTCTGACCAGCCAGGCCCTAAGAAAAAATTTAAAGCTCCCCAGAGC AAGGCCTTGGCAGCCAGCTTTTCAGAGGGAAAAGAAGTTAGAACAGTGCCAAAGAACCTGGGCCTATCA GCGTGTAAGGGGGCCTGCCCCTCATCTGCGAAAGAAGAGCCCAGACACCGGGAACCCCTGACCCAGGAG GCTGGAAGTGTCCCCCTTGAGGACGAAGCCTCCAGTGACCTGGACCTGGAGCAACTCATGGAAGATGTT GGGAGAGAGCTGGGGCAGAGCGGGGAGCTGCAGCACAGCAACAGTGATGGCGAGGACTTCCCCGTGGCG CTGTTTGGGAAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_017984 |
| Insert Size | 1947 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_017984.4 |
| RefSeq Size | 2485 bp |
| RefSeq ORF | 1947 bp |
| Locus ID | 55063 |
| UniProt ID | Q9H0M4 |
| Domains | PWWP |
| MW | 72 kDa |
| Gene Summary | Dual histone methylation reader specific for PRDM9-catalyzed histone marks (H3K4me3 and H3K36me3) (PubMed:32744506, PubMed:20826339). Facilitates the repair of PRDM9-induced meiotic double-strand breaks (DSBs) (By similarity). Essential for male fertility and spermatogenesis (By similarity). Required for meiosis prophase I progression in male but not in female germ cells (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214455 | ZCWPW1 (Myc-DDK-tagged)-Human zinc finger, CW type with PWWP domain 1 (ZCWPW1) |
CN¥ 4,824.00 |
|
| RC214455L3 | Lenti ORF clone of Human zinc finger, CW type with PWWP domain 1 (ZCWPW1), Myc-DDK-tagged |
CN¥ 6,460.00 |
|
| RC214455L4 | Lenti ORF clone of Human zinc finger, CW type with PWWP domain 1 (ZCWPW1), mGFP tagged |
CN¥ 6,460.00 |
|
| RG214455 | ZCWPW1 (tGFP-tagged) - Human zinc finger, CW type with PWWP domain 1 (ZCWPW1) |
CN¥ 5,040.00 |
