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DDX6 (NM_004397) Human Untagged Clone
CAT＃: SC317807
DDX6 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 (DDX6)
CN¥ 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HLR2; IDDILF; P54; RCK; Rck/p54 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC317807 representing NM_004397.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGCACGGCCAGAACAGAGAACCCTGTTATAATGGGTCTGTCCAGTCAAAATGGTCAGCTGAGAGGC CCTGTGAAACCCACTGGTGGCCCTGGAGGAGGGGGCACACAGACACAGCAACAGATGAACCAGCTGAAA AACACCAACACAATCAATAATGGCACTCAGCAGCAAGCACAGAGTATGACCACCACTATTAAACCTGGT GATGACTGGAAAAAGACTTTAAAACTCCCTCCAAAGGATCTAAGAATCAAAACTTCGGATGTGACCTCC ACAAAAGGAAATGAGTTTGAAGATTACTGTTTGAAACGGGAGTTACTGATGGGAATTTTTGAAATGGGC TGGGAAAAGCCATCTCCTATTCAGGAGGAGAGCATTCCCATTGCTTTATCTGGTAGGGATATCTTAGCT AGAGCAAAAAATGGAACAGGCAAGAGCGGTGCCTACCTCATTCCCTTACTTGAACGGCTAGACCTGAAG AAGGACAATATACAAGCAATGGTGATTGTTCCCACTAGAGAACTTGCTCTACAGGTCAGTCAAATTTGC ATCCAGGTCAGCAAACACATGGGAGGGGCCAAAGTGATGGCAACCACAGGAGGAACCAATTTACGAGAT GACATAATGAGGCTTGATGATACAGTGCACGTGGTGATTGCTACCCCTGGGAGAATCCTGGATCTTATT AAGAAAGGAGTAGCAAAGGTTGATCATGTCCAGATGATAGTATTGGATGAGGCAGATAAGTTGCTGTCA CAGGATTTTGTGCAGATAATGGAGGATATTATTCTCACGCTACCTAAAAACAGGCAGATTTTACTATAT TCCGCTACTTTCCCTCTTAGTGTACAGAAGTTCATGAATTCCCATTTGCAGAAACCCTATGAGATTAAC CTGATGGAGGAACTAACTCTGAAGGGAGTAACCCAGTACTACGCATATGTAACTGAGCGCCAAAAAGTA CACTGCCTCAACACACTTTTCTCCAGGCTTCAGATAAACCAGTCGATCATTTTCTGTAACTCCTCTCAG CGAGTTGAATTGCTAGCCAAGAAGATTTCTCAACTGGGTTATTCTTGCTTCTATATTCATGCTAAAATG AGGCAGGAACATCGAAATCGTGTATTTCATGATTTCCGAAATGGCTTATGCCGCAATCTTGTTTGCACT GATCTGTTTACCCGAGGTATTGATATACAAGCTGTGAATGTGGTAATAAACTTTGATTTCCCAAAGCTG GCAGAGACCTATCTCCATCGTATTGGAAGATCAGGTCGCTTTGGTCATCTTGGCTTAGCCATCAACTTG ATCACATATGATGATCGCTTCAACCTGAAAAGTATTGAGGAGCAGCTGGGAACAGAAATTAAACCTATT CCGAGCAACATTGATAAGAGCCTGTATGTGGCAGAATACCACAGCGAGCCTGTAGAAGATGAGAAACCT TAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_004397 |
| Insert Size | 1452 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004397.5 |
| RefSeq Size | 6246 bp |
| RefSeq ORF | 1452 bp |
| Locus ID | 1656 |
| UniProt ID | P26196 |
| Domains | DEAD, helicase_C |
| Protein Pathways | RNA degradation |
| MW | 54.4 kDa |
| Gene Summary | This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Mar 2012] Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209431 | DDX6 (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 (DDX6) |
CN¥ 3,656.00 |
|
| RC209431L1 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 (DDX6), Myc-DDK-tagged |
CN¥ 6,056.00 |
|
| RC209431L2 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 (DDX6), mGFP tagged |
CN¥ 5,890.00 |
|
| RC209431L3 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 (DDX6), Myc-DDK-tagged |
CN¥ 6,056.00 |
|
| RC209431L4 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 (DDX6), mGFP tagged |
CN¥ 6,056.00 |
|
| RG209431 | DDX6 (tGFP-tagged) - Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 (DDX6) |
CN¥ 5,256.00 |
|
| SC117398 | DDX6 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 (DDX6) |
CN¥ 3,656.00 |
