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SUCLG2 (NM_003848) Human Untagged Clone
CAT＃: SC317700
SUCLG2 (untagged)-Human succinate-CoA ligase, GDP-forming, beta subunit (SUCLG2), nuclear gene encoding mitochondrial protein, transcript variant 2
CN¥ 7,220.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | G-SCS; GBETA; GTPSCS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC317700 representing NM_003848.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGTCCCCCGTAGCAGCGCAGGCCGGGAAGCTTCTGCGAGCCCTAGCGCTGCGGCCCCGCTTCCTG GCGGCCGGGTCCCAGGCAGTTCAATTAACCTCCAGAAGATGGCTGAACCTGCAGGAATACCAGAGCAAG AAACTGATGTCTGACAACGGAGTGAGAGTTCAAAGATTCTTTGTAGCAGACACTGCAAATGAAGCTCTC GAGGCTGCTAAGAGACTAAATGCAAAAGAAATTGTTTTAAAAGCCCAGATCTTAGCTGGAGGAAGAGGA AAAGGTGTCTTCAATAGTGGTTTGAAAGGAGGTGTTCATTTAACAAAAGACCCTAATGTTGTGGGACAG CTGGCTAAACAGATGATTGGGTACAATCTAGCGACAAAACAAACTCCAAAAGAAGGTGTGAAAGTTAAC AAGGTGATGGTTGCTGAAGCCTTGGATATTTCCAGAGAAACCTACCTGGCAATTCTGATGGACCGGTCC TGCAATGGCCCCGTGCTGGTGGGCAGCCCCCAGGGGGGCGTCGACATTGAAGAGGTGGCTGCTTCAAAC CCGGAGCTCATTTTTAAGGAGCAAATTGACATTTTTGAAGGAATAAAGGACAGCCAAGCTCAGCGGATG GCCGAAAATCTAGGCTTCGTTGGGCCTTTGAAAAGCCAGGCTGCAGATCAAATTACGAAGCTGTATAAT CTCTTCCTGAAAATTGATGCTACTCAGGTGGAAGTGAATCCCTTTGGTGAAACTCCAGAAGGACAAGTT GTCTGTTTTGATGCCAAGATAAACTTTGATGACAACGCAGAATTCCGACAAAAAGACATATTTGCTATG GACGACAAATCAGAGAATGAGCCCATTGAAAATGAAGCTGCCAAATATGATCTAAAATACATAGGACTA GATGGGAACATTGCCTGCTTTGTGAATGGTGCTGGGCTCGCCATGGCTACTTGTGATATCATTTTCCTT AATGGTGGGAAGCCAGCCAACTTCTTGGATCTTGGAGGTGGTGTAAAGGAAGCTCAAGTATATCAAGCA TTCAAATTGCTCACAGCTGATCCTAAGGTTGAAGCCATCCTTGTCAATATATTTGGTGGTATCGTCAAC TGTGCCATCATTGCCAATGGGATCACCAAAGCCTGCCGGGAGCTAGAACTCAAGGTGCCCCTGGTGGTC CGGCTTGAAGGAACCAACGTCCAAGAGGCCCAGAAGATACTCAACAACAGCGGACTCCCCATTACTTCA GCCATTGACCTGGAGGATGCAGCCAAGAAGGCTGTGGCCAGTGTGGCCAAGAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_003848 |
| Insert Size | 1299 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003848.3 |
| RefSeq Size | 2363 bp |
| RefSeq ORF | 1299 bp |
| Locus ID | 8801 |
| UniProt ID | Q96I99 |
| Protein Pathways | Citrate cycle (TCA cycle), Metabolic pathways, Propanoate metabolism |
| MW | 46.5 kDa |
| Gene Summary | This gene encodes a GTP-specific beta subunit of succinyl-CoA synthetase. Succinyl-CoA synthetase catalyzes the reversible reaction involving the formation of succinyl-CoA and succinate. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 5 and 12. [provided by RefSeq, Apr 2010] Transcript Variant: This variant (2) differs in the 3' UTR, and 3' coding region, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Mitochondrial GTP Links Nutrient Sensing to β Cell Health, Mitochondrial Morphology, and Insulin Secretion Independent of OxPhos
,Jesinkey, SR;Madiraju, AK;Alves, TC;Yarborough, OH;Cardone, RL;Zhao, X;Parsaei, Y;Nasiri, AR;Butrico, G;Liu, X;Molina, AJ;Rountree, AM;Neal, AS;Wolf, DM;Sterpka, J;Philbrick, WM;Sweet, IR;Shirihai, OH;Kibbey, RG;,
Cell Rep
,PubMed ID 31315053
[SUCLG2]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208298 | SUCLG2 (Myc-DDK-tagged)-Human succinate-CoA ligase, GDP-forming, beta subunit (SUCLG2), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CN¥ 3,656.00 |
|
| RC208298L3 | Lenti ORF clone of Human succinate-CoA ligase, GDP-forming, beta subunit (SUCLG2), nuclear gene encoding mitochondrial protein, transcript variant 2, Myc-DDK-tagged |
CN¥ 5,890.00 |
|
| RC208298L4 | Lenti ORF clone of Human succinate-CoA ligase, GDP-forming, beta subunit (SUCLG2), nuclear gene encoding mitochondrial protein, transcript variant 2, mGFP tagged |
CN¥ 5,890.00 |
|
| RG208298 | SUCLG2 (tGFP-tagged) - Human succinate-CoA ligase, GDP-forming, beta subunit (SUCLG2), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CN¥ 5,256.00 |
|
| SC321824 | SUCLG2 (untagged)-Human succinate-CoA ligase, GDP-forming, beta subunit (SUCLG2), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CN¥ 3,656.00 |
