BMT2 (NM_152556) Human Untagged Clone

CAT#: SC317648

C7orf60 (untagged)-Human chromosome 7 open reading frame 60 (C7orf60)



  "NM_152556" in other vectors (4)

CNY 7,220.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms C7orf60; SAMTOR
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC317648 representing NM_152556.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGAGCCAGGGGCCGGCGGCCGAAATACTGCTCGTGCGCAGAGGGCCGGGTCCCCGAACACTCCGCCG
CCCCGGGAGCAGGAGCGGAAACTGGAGCAGGAGAAGCTCTCCGGTGTGGTGAAGAGCGTCCACCGGCGG
CTCCGCAAGAAGTACCGAGAAGTGGGAGATTTTGATAAGATCTGGCGAGAACACTGTGAGGATGAAGAA
ACTCTTTGTGAATATGCCGTTGCAATGAAAAATTTGGCAGATAATCATTGGGCAAAAACTTGTGAGGGC
GAAGGTCGTATTGAATGGTGTTGTAGTGTATGCAGAGAATATTTCCAAAATGGTGGGAAGAGAAAAGCA
CTTGAGAAAGATGAAAAAAGAGCTGTACTTGCCACTAAGACCACTCCAGCCTTAAATATGCATGAGTCT
TCTCAACTTGAAGGTCATTTAACCAACCTCAGCTTTACAAACCCTGAATTTATAACTGAGTTGCTACAA
GCCTCAGGAAAAATCAGATTACTTGATGTTGGCAGCTGCTTTAACCCATTTCTGAAGTTTGAAGAATTT
CTAACTGTTGGCATAGATATTGTACCTGCTGTAGAGAGTGTCTATAAATGTGATTTCCTGAACTTACAG
CTTCAGCAACCACTCCAGCTTGCACAGGATGCTATAGATGCTTTTTTGAAGCAGCTGAAAAACCCTATT
GATTCTCTTCCTGGAGAGCTTTTCCATGTGGTTGTTTTCTCTCTCCTTCTTTCTTATTTTCCATCTCCT
TACCAGCGATGGATTTGCTGCAAGAAAGCCCATGAACTGTTAGTGTTAAATGGTTTATTACTAATCATC
ACACCTGATTCCTCCCATCAGAACCGTCATGCTATGATGATGAAAAGCTGGAAGATTGCTATAGAGTCC
CTGGGCTTTAAACGCTTCAAGTACTCAAAATTTTCACATATGCATCTGATGGCATTTAGGAAAATCTCT
CTAAAAACCACAAGTGACTTGGTTAGTAGGAACTACCCAGGAATGTTATATATTCCTCAAGATTTCAAC
AGTATAGAAGATGAGGAATATTCTAACCCTTCCTGCTATGTTCGATCAGATATAGAAGATGAACAACTA
GCATATGGTTTCACAGAACTCCCTGATGCGCCATATGACTCAGATTCTGGAGAAAGTCAAGCCAGCTCT
ATTCCTTTCTATGAGCTAGAAGACCCCATATTACTTTTAAGTTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_152556
Insert Size 1218 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_152556.2
RefSeq Size 3969 bp
RefSeq ORF 1218 bp
Locus ID 154743
UniProt ID Q1RMZ1
MW 46.3 kDa
Gene Summary S-adenosyl-L-methionine-binding protein that acts as an inhibitor of mTORC1 signaling via interaction with the GATOR1 and KICSTOR complexes (PubMed:29123071). Acts as a sensor of S-adenosyl-L-methionine to signal methionine sufficiency to mTORC1: in presence of methionine, binds S-adenosyl-L-methionine, leading to disrupt interaction with the GATOR1 and KICSTOR complexes and promote mTORC1 signaling (PubMed:29123071). Upon methionine starvation, S-adenosyl-L-methionine levels are reduced, thereby promoting the association with GATOR1 and KICSTOR, leading to inhibit mTORC1 signaling (PubMed:29123071). Probably also acts as a S-adenosyl-L-methionine-dependent methyltransferase (Potential).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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