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PCGF1 (NM_032673) Human Untagged Clone
CAT＃: SC317369
PCGF1 (untagged)-Human polycomb group ring finger 1 (PCGF1)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2010002K04Rik; NSPC1; RNF3A-2; RNF68 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC317369 representing NM_032673.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTA CCGAGGAGATCTGCCGCCGCGATCGCCGGCGCGCCC ATGGCGTCTCCTCAGGGGGGCCAGATTGCGATCGCGATGAGGCTTCGGAACCAGCTCCAGTCAGTGTAC AAGATGGACCCGCTACGGAACGAGGAGGAGGTTCGAGTGAAGATCAAAGACTTGAATGAACACATTGTT TGCTGCCTATGCGCCGGCTACTTCGTGGATGCCACCACCATCACAGAGTGTCTTCATACTTTCTGCAAG AGTTGTATTGTGAAGTACCTCCAAACTAGCAAGTACTGCCCCATGTGCAACATTAAGATCCACGAGACA CAGCCACTGCTCAACCTCAAACTGGACCGGGTCATGCAGGACATCGTGTATAAGCTGGTGCCTGGCTTG CAAGACAGTGAAGAGAAACGGATTCGGGAATTCTACCAGTCCCGAGGTTTGGACCGGGTCACCCAGCCC ACTGGGGAAGAGCCAGCACTGAGCAACCTCGGCCTCCCCTTCAGCAGCTTTGACCACTCTAAAGCCCAC TACTATCGCTATGATGAGCAGTTGAACCTGTGCCTGGAGCGGCTGAGTTCTGGCAAAGACAAGAATAAA AGCGTCCTGCAGAACAAGTATGTCCGATGTTCTGTTAGAGCTGAGGTACGCCATCTCCGGAGGGTCCTG TGTCACCGCTTGATGCTAAACCCTCAGCATGTGCAGCTCCTTTTTGACAATGAAGTTCTCCCTGATCAC ATGACAATGAAGCAGATATGGCTCTCCCGCTGGTTCGGCAAGCCATCCCCTTTGCTTTTACAATACAGT GTGAAAGAGAAGAGGAGGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | AscI-MluI |
| ACCN | NM_032673 |
| Insert Size | 780 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_032673.2 |
| RefSeq Size | 939 bp |
| RefSeq ORF | 780 bp |
| Locus ID | 84759 |
| UniProt ID | Q9BSM1 |
| Domains | RING |
| MW | 30.3 kDa |
| Gene Summary | PCGF1 is a mammalian homolog of the Drosophila polycomb group genes, which act as transcriptional repressors to regulate anterior-posterior patterning in early embryonic development (Nunes et al., 2001 [PubMed 11287196]). See also PCGF2 (MIM 600346).[supplied by OMIM, Aug 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216322 | PCGF1 (Myc-DDK-tagged)-Human polycomb group ring finger 1 (PCGF1) |
CNY 2,400.00 |
|
| RC216322L1 | Lenti ORF clone of Human polycomb group ring finger 1 (PCGF1), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC216322L2 | Lenti ORF clone of Human polycomb group ring finger 1 (PCGF1), mGFP tagged |
CNY 5,890.00 |
|
| RC216322L3 | Lenti ORF clone of Human polycomb group ring finger 1 (PCGF1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC216322L4 | Lenti ORF clone of Human polycomb group ring finger 1 (PCGF1), mGFP tagged |
CNY 5,890.00 |
|
| RG216322 | PCGF1 (tGFP-tagged) - Human polycomb group ring finger 1 (PCGF1) |
CNY 4,370.00 |
|
| SC103969 | PCGF1 (untagged)-Human polycomb group ring finger 1 (PCGF1) |
CNY 2,400.00 |
