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DROSHA (NM_001100412) Human Untagged Clone
CAT＃: SC316870
DROSHA (untagged)-Human drosha, ribonuclease type III (DROSHA), transcript variant 2
CNY 25,750.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ETOHI2; HSA242976; RANSE3L; RN3; RNASE3L; RNASEN |
| Vector | pCMV6 series |
| Sequence Data |
>NCBI ORF sequence for NM_001100412, the custom clone sequence may differ by one or more nucleotides
ATGATGCAGGGAAACACATGTCACAGAATGTCGTTCCACCCGGGACGAGGGTGTCCCCGA GGACGAGGAGGACATGGAGCCAGACCCTCAGCACCATCCTTTAGGCCCCAAAATCTGAGG CTGCTTCACCCTCAGCAGCCTCCTGTGCAATATCAATATGAACCTCCAAGTGCCCCTTCC ACCACTTTCTCAAACTCTCCAGCCCCCAATTTTCTCCCTCCACGACCAGACTTTGTACCC TTCCCCCCACCCATGCCTCCGTCAGCGCAAGGCCCTCTTCCCCCCTGCCCAATCAGGCCG CCTTTCCCCAACCACCAGATGAGGCACCCCTTCCCAGTTCCTCCTTGTTTTCCTCCCATG CCACCACCAATGCCTTGTCCTAATAACCCCCCAGTCCCTGGGGCACCTCCTGGACAAGGC ACTTTCCCCTTCATGATGCCCCCTCCCTCCATGCCTCATCCCCCGCCCCCTCCAGTCATG CCGCAGCAGGTTAATTATCAGTACCCTCCGGGCTATTCTCACCACAACTTCCCACCTCCC AGTTTTAATAGTTTCCAGAACAACCCTAGTTCTTTCCTGCCCAGTGCTAATAACAGCAGT AGTCCTCATTTCAGACATCTCCCTCCATACCCACTCCCAAAGGCTCCCAGTGAGAGAAGG TCCCCAGAAAGGCTGAAACACTATGATGACCACAGGCACCGAGATCACAGTCATGGGCGA GGTGAGAGGCATCGGTCCCTGGATCGGCGGGAGCGAGGCCGCAGTCCCGACAGGAGAAGA CAAGACAGCCGGTACAGATCTGATTATGACCGAGGGAGAACACCATCTCGCCACCGCAGC TACGAACGGAGCAGAGAGCGAGAACGGGAGAGACACAGGCATCGAGACAACCGAAGATCA CCATCTCTGGAAAGGTCCTACAAAAAAGAGTATAAGAGATCTGGAAGTCGCTCCCCAAGT AGGGAGAAGAAGAGAGCTCGTTGGGAGGAAGAAAAAGACCGTTGGAGTGACAACCAGAGT TCTGGCAAAGACAAGAACTATACCTCAATCAAGGAAAAAGAGCCCGAGGAGACCATGCCT GACAAGAATGAGGAGGAAGAAGAAGAACTTCTTAAGCCTGTGTGGATTCGATGCACTCAT TCAGAAAACTACTACTCCAGTGACCCCATGGATCAGGTGGGAGATTCTACAGTGGTTGGA ACGAGTAGGCTTCGTGACTTATATGACAAATTTGAGGAGGAGTTGGGGAGCAGGCAAGAA AAGGCCAAAGCTGCTCGGCCTCCGTGGGAACCTCCAAAGACGAAGCTCGATGAAGATTTA GAGAGTTCCAGTGAATCCGAGTGTGAGTCTGATGAGGACAGCACCTGTTCTAGCAGCTCA GACTCTGAAGTTTTTGACGTTATTGCAGAAATCAAACGCAAAAAGGCCCACCCTGACCGA CTTCATGATGAACTTTGGTACAACGATCCAGGCCAGATGAATGATGGACCACTCTGCAAA TGCAGCGCAAAGGCAAGACGCACAGGAATTAGGCACAGCATTTATCCTGGAGAAGAGGCC ATCAAGCCCTGTCGTCCTATGACCAACAATGCTGGCAGACTTTTCCACTACCGGATCACA GTCTCCCCGCCTACGAACTTTTTAACTGACAGGCCAACTGTTATAGAATACGATGATCAC GAGTATATCTTTGAAGGATTTTCTATGTTTGCACATGCCCCCCTGACCAATATTCCACTG TGTAAAGTAATTAGATTCAACATAGACTACACGATTCATTTCATTGAAGAGATGATGCCG GAGAATTTTTGTGTGAAAGGGCTTGAACTCTTTTCACTGTTCCTATTCAGAGATATTTTG GAATTATATGACTGGAATCTTAAAGGTCCTTTGTTTGAAGACAGCCCTCCCTGCTGCCCA AGATTTCATTTCATGCCACGTTTTGTAAGATTTCTTCCAGATGGAGGAAAGGAAGTGCTG TCCATGCACCAGATTCTCCTGTACTTGTTAAGGTGCAGCAAAGCCCTGGTGCCTGAGGAG GAGATTGCCAATATGCTTCAGTGGGAGGAGCTGGAGTGGCAGAAATATGCAGAAGAATGC AAAGGCATGATTGTTACCAACCCTGGGACGAAACCAAGCTCTGTCCGTATCGATCAACTG GATCGTGAACAGTTCAACCCCGATGTGATTACTTTTCCGATTATCGTCCACTTTGGGATA CGCCCTGCACAGTTGAGTTATGCAGGAGACCCACAGTACCAAAAACTGTGGAAGAGTTAT GTGAAACTTCGCCACCTCCTAGCAAATAGTCCCAAAGTCAAACAAACTGACAAACAGAAG CTGGCACAGAGGGAGGAAGCCCTCCAAAAAATACGGCAGAAGAATACAATGAGACGAGAA GTAACGGTGGAGCTAAGTAGCCAAGGATTCTGGAAAACTGGCATCCGTTCTGATGTCTGT CAGCATGCAATGATGCTACCTGTTCTGACCCATCATATCCGCTACCACCAATGCCTAATG CATTTGGACAAGTTGATAGGATATACTTTCCAAGATCGTTGTCTGTTGCAGCTGGCCATG ACTCATCCAAGTCATCATTTAAATTTTGGAATGAATCCTGATCATGCCAGGAATTCATTA TCTAACTGTGGAATTCGGCAGCCCAAATACGGAGACAGAAAAGTTCATCACATGCACATG CGGAAGAAAGGGATTAACACCTTGATAAATATCATGTCACGCCTTGGCCAAGATGACCCA ACTCCCTCGAGGATTAACCACAATGAACGGTTGGAATTCCTGGGTGATGCTGTTGTTGAA TTTCTGACCAGCGTCCATTTGTACTATTTGTTTCCTAGTCTGGAAGAAGGAGGATTAGCA ACCTATCGGACTGCCATTGTTCAGAATCAGCACCTTGCCATGCTAGCAAAGAAACTTGAA CTGGATCGATTTATGCTGTATGCTCACGGGCCTGACCTTTGTAGAGAATCGGACCTTCGA CATGCAATGGCCAATTGTTTTGAAGCGTTAATAGGAGCTGTTTACTTGGAGGGAAGCCTG GAGGAAGCCAAGCAGTTATTTGGACGCTTGCTCTTTAATGATCCGGACCTGCGCGAAGTC TGGCTCAATTATCCTCTCCACCCACTCCAACTACAAGAGCCAAATACTGATCGACAACTT ATTGAAACTTCTCCAGTTCTACAAAAACTTACTGAGTTTGAAGAAGCAATTGGAGTAATT TTTACTCATGTTCGACTTCTGGCAAGGGCATTCACATTGAGAACTGTGGGATTTAACCAT CTGACCCTAGGCCACAATCAGAGAATGGAATTCCTAGGTGACTCCATAATGCAACTGGTA GCCACAGAGTACTTATTCATTCATTTCCCAGATCATCATGAAGGACACTTAACTTTGTTG CGAAGCTCTTTGGTGAATAATAGAACTCAGGCCAAGGTAGCGGAGGAGCTGGGCATGCAG GAGTACGCCATAACCAACGACAAGACCAAGAGGCCTGTGGCGCTTCGCACCAAGACCTTG GCGGACCTTTTGGAATCATTTATTGCAGCGCTGTACATTGATAAGGATTTGGAATATGTT CATACTTTCATGAATGTCTGCTTCTTTCCACGATTGAAAGAGTTCATTTTGAATCAGGAT TGGAATGACCCCAAATCCCAGCTTCAGCAGTGTTGCTTGACACTTAGGACAGAAGGAAAA GAGCCAGACATTCCTCTGTACAAGACTCTGCAGACAGTGGGCCCATCCCATGCCCGAACC TACACTGTGGCTGTTTATTTCAAGGGAGAAAGAATAGGCTGTGGGAAAGGACCAAGTATT CAGCAAGCGGAAATGGGAGCAGCAATGGATGCGCTTGAAAAATATAATTTTCCCCAGATG GCCCATCAGAAGCGGTTCATCGAACGGAAGTACAGACAAGAGTTAAAAGAAATGAGGTGG GAAAGAGAGCATCAAGAGAGAGAGCCAGATGAGACTGAAGACATCAAGAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_001100412 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001100412.1, NP_001093882.1 |
| RefSeq Size | 5389 bp |
| RefSeq ORF | 4014 bp |
| Locus ID | 29102 |
| UniProt ID | Q9NRR4 |
| Gene Summary | This gene encodes a ribonuclease (RNase) III double-stranded RNA-specific ribonuclease and subunit of the microprocessor protein complex, which catalyzes the initial processing step of microRNA (miRNA) synthesis. The encoded protein cleaves the stem loop structure from the primary microRNA (pri-miRNA) in the nucleus, yielding the precursor miRNA (pre-miRNA), which is then exported to the cytoplasm for further processing. In a human cell line lacking a functional copy of this gene, canonical miRNA synthesis is reduced. Somatic mutations in this gene have been observed in human patients with kidney cancer. [provided by RefSeq, Sep 2016] Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. The resulting protein (isoform 2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217782 | DROSHA (Myc-DDK-tagged)-Human drosha, ribonuclease type III (DROSHA), transcript variant 2 |
CNY 13,300.00 |
|
| RC217782L3 | Lenti-ORF clone of DROSHA (Myc-DDK-tagged)-Human drosha, ribonuclease type III (DROSHA), transcript variant 2 |
CNY 15,200.00 |
|
| RC217782L4 | Lenti-ORF clone of DROSHA (mGFP-tagged)-Human drosha, ribonuclease type III (DROSHA), transcript variant 2 |
CNY 15,200.00 |
|
| RG217782 | DROSHA (tGFP-tagged) - Human drosha, ribonuclease type III (DROSHA), transcript variant 2 |
CNY 14,630.00 |
