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USP22 (NM_015276) Human Untagged Clone
CAT＃: SC316543
USP22 (untagged)-Human ubiquitin specific peptidase 22 (USP22)
CN¥ 5,872.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | USP3L |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_015276 edited
ATGGTGTCCCGGCCAGAGCCCGAGGGCGAGGCCATGGACGCCGAGCTGGCGGTAGCGCCG CCGGGCTGCTCGCACCTGGGCAGCTTCAAGGTGGACAACTGGAAGCAGAACCTGCGGGCC ATCTACCAGTGCTTCGTGTGGAGCGGCACGGCTGAGGCCCGCAAGCGCAAGGCCAAGTCC TGTATCTGCCATGTCTGTGGCGTCCACCTCAACAGGCTGCATTCCTGCCTCTACTGTGTC TTCTTCGGCTGTTTCACAAAGAAGCATATTCACGAGCATGCGAAGGCGAAGCGGCACAAC CTGGCCATTGATCTGATGTATGGAGGCATCTACTGTTTTCTGTGCCAGGACTACATCTAT GACAAAGACATGGAAATAATCGCCAAGGAGGAGCAGCGAAAAGCTTGGAAAATGCAAGGC GTTGGAGAGAAGTTTTCAACTTGGGAACCAACCAAACGGGAGCTTGAACTGCTGAAGCAC AACCCGAAAAGGAGAAAGATCACCTCGAACTGCACCATAGGTCTGCGTGGGCTGATCAAC CTTGGGAACACATGCTTCATGAACTGCATCGTGCAGGCCCTGACCCACACGCCACTTCTG CGGGACTTCTTCCTGTCTGACAGGCACCGCTGTGAGATGCAGAGCCCCAGCTCCTGTCTG GTCTGTGAGATGTCCTCACTGTTTCAGGAGTTTTACTCTGGACACCGGTCCCCTCACATC CCGTATAAGTTGCTGCACCTGGTGTGGACCCACGCGAGGCACCTAGCAGGCTACGAGCAG CAGGACGCCCACGAGTTCCTCATCGCGGCCCTGGACGTGCTCCACCGACACTGCAAAGGT GATGACAATGGGAAGAAGGCCAACAACCCCAACCACTGCAACTGCATCATAGACCAGATC TTCACAGGCGGGTTGCAGTCAGACGTCACCTGCCAAGTCTGCCATGGAGTCTCCACCACC ATCGACCCCTTCTGGGACATCAGCTTGGATCTCCCCGGCTCTTCCACCCCATTCTGGCCC CTGAGCCCAGGGAGCGAGGGCAACGTGGTAAACGGGGAAAGCCACGTGTCGGGAACCACC ACGCTCACGGACTGCCTGCGACGATTCACCAGACCAGAGCACTTGGGCAGCAGCGCCAAG ATCAAGTGCAGCGGTTGCCATAGCTACCAGGAGTCCACAAAGCAGCTCACTATGAAGAAA CTGCCCATCGTAGCCTGTTTTCATCTCAAACGATTTGAACACTCAGCCAAGCTGCGGCGG AAGATCACCACGTATGTGTCCTTCCCCCTGGAGCTGGACATGACCCCTTTCATGGCCTCC AGCAAAGAGAGCAGGATGAATGGACAGTACCAGCAGCCCACGGACAGTCTCAACAATGAC AACAAGTATTCCCTGTTTGCTGTTGTTAACCATCAAGGGACCTTGGAGAGTGGCCACTAC ACCAGCTTTATCCGGCAGCACAAAGACCAGTGGTTCAAGTGTGACGATGCCATCATCACC AAGGCCAGCATCAAGGACGTCCTGGACAGCGAAGGGTACTTGCTGTTCTATCACAAACAG TTCCTGGAATACGAGTAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_015276 |
| Insert Size | 5000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | ORF was fully sequenced and the sequence matches with that of NM_015276.1.One SNP was observed in the ORF, which does not change amino acid. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015276.1, NP_056091.1 |
| RefSeq Size | 5220 bp |
| RefSeq ORF | 1578 bp |
| Locus ID | 23326 |
| UniProt ID | Q9UPT9 |
| Protein Families | Protease |
| Gene Summary | Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation and cell cycle progression.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
USP22 acts as an oncogene by regulating the stability of cyclooxygenase-2 in non-small cell lung cancer
,Xiao, H;Tian, Y;Yang, Y;Hu, F;Xie, X;Mei, J;Ding, F;,
Biochem. Biophys. Res. Commun.
,PubMed ID 25817787
[USP22]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219906 | USP22 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 22 (USP22) |
CN¥ 5,864.00 |
|
| RC219906L1 | Lenti ORF clone of Human ubiquitin specific peptidase 22 (USP22), Myc-DDK-tagged |
CN¥ 8,264.00 |
|
| RC219906L2 | Lenti ORF clone of Human ubiquitin specific peptidase 22 (USP22), mGFP tagged |
CN¥ 6,460.00 |
|
| RC219906L3 | Lenti ORF clone of Human ubiquitin specific peptidase 22 (USP22), Myc-DDK-tagged |
CN¥ 6,460.00 |
|
| RC219906L4 | Lenti ORF clone of Human ubiquitin specific peptidase 22 (USP22), mGFP tagged |
CN¥ 8,264.00 |
|
| RG219906 | USP22 (tGFP-tagged) - Human ubiquitin specific peptidase 22 (USP22) |
CN¥ 7,464.00 |
