EIF4E1B (NM_001099408) Human Untagged Clone
CAT#: SC316492
EIF4E1B (untagged)-Human eukaryotic translation initiation factor 4E family member 1B (EIF4E1B)
CNY 3,990.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>SC316492 representing NM_001099408.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCTTGCTGTTGAGGTGAGTGAAGCTGAGGGTGGAATCCGAGAGTGGGAGGAGGAGGAGAAGGAGGAG GAGGCAGCAGAGAGGACGCCCACAGGAGAAAAGTCTCCAAACTCTCCCAGGACTTTGCTGTCTCTGAGA GGGAAGGCCCGGACGGGGGGCCCCATGGAAGTCAAGCTGGAGCTGCACCCCTTGCAGAACAGGTGGGCT CTGTGGTTCTTCAAGAATGACCGCAGCCGGGCCTGGCAGGACAACCTGCACCTGGTCACCAAGGTGGAC ACTGTGGAGGACTTCTGGGCGCTATACAGTCACATCCAGCTGGCCAGCAAGCTCTCCTCTGGCTGTGAC TACGCCCTCTTCAAGGATGGCATCCAGCCCATGTGGGAGGACAGCAGGAATAAACGGGGTGGCCGCTGG CTGGTCAGCCTGGCCAAGCAGCAGCGCCACATTGAGCTGGACCGGCTGTGGCTGGAGACGCTGCTGTGT CTGATCGGGGAGAGCTTTGAGGAACACAGCAGAGAGGTATGTGGGGCCGTCGTCAACATCCGCACCAAG GGGGACAAGATCGCTGTGTGGACGAGGGAGGCGGAAAACCAGGCGGGCGTGCTGCACGTTGGGCGTGTA TACAAAGAGCGCCTGGGCCTCTCCCCAAAGACCATCATTGGGTACCAGGCCCATGCAGACACAGCCACC AAGAGCAACTCCCTAGCCAAGAACAAGTTTGTGGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
Restriction Sites | SgfI-MluI |
ACCN | NM_001099408 |
Insert Size | 729 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001099408.1 |
RefSeq Size | 1974 bp |
RefSeq ORF | 729 bp |
Locus ID | 253314 |
UniProt ID | A6NMX2 |
Protein Pathways | Insulin signaling pathway, mTOR signaling pathway |
MW | 27.6 kDa |
Gene Summary | Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structure.[UniProtKB/Swiss-Prot Function] |
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Other Versions
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RC219823 | EIF4E1B (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4E family member 1B (EIF4E1B) |
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RC219823L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 4E family member 1B (EIF4E1B), Myc-DDK-tagged |
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RC219823L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 4E family member 1B (EIF4E1B), mGFP tagged |
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RG219823 | EIF4E1B (tGFP-tagged) - Human eukaryotic translation initiation factor 4E family member 1B (EIF4E1B) |
CNY 4,370.00 |
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