EIF4E1B (NM_001099408) Human Untagged Clone

CAT#: SC316492

EIF4E1B (untagged)-Human eukaryotic translation initiation factor 4E family member 1B (EIF4E1B)



  "NM_001099408" in other vectors (4)

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-EIF4E1B Antibody
    • 100 ul

CNY 5,250.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC316492 representing NM_001099408.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCTTGCTGTTGAGGTGAGTGAAGCTGAGGGTGGAATCCGAGAGTGGGAGGAGGAGGAGAAGGAGGAG
GAGGCAGCAGAGAGGACGCCCACAGGAGAAAAGTCTCCAAACTCTCCCAGGACTTTGCTGTCTCTGAGA
GGGAAGGCCCGGACGGGGGGCCCCATGGAAGTCAAGCTGGAGCTGCACCCCTTGCAGAACAGGTGGGCT
CTGTGGTTCTTCAAGAATGACCGCAGCCGGGCCTGGCAGGACAACCTGCACCTGGTCACCAAGGTGGAC
ACTGTGGAGGACTTCTGGGCGCTATACAGTCACATCCAGCTGGCCAGCAAGCTCTCCTCTGGCTGTGAC
TACGCCCTCTTCAAGGATGGCATCCAGCCCATGTGGGAGGACAGCAGGAATAAACGGGGTGGCCGCTGG
CTGGTCAGCCTGGCCAAGCAGCAGCGCCACATTGAGCTGGACCGGCTGTGGCTGGAGACGCTGCTGTGT
CTGATCGGGGAGAGCTTTGAGGAACACAGCAGAGAGGTATGTGGGGCCGTCGTCAACATCCGCACCAAG
GGGGACAAGATCGCTGTGTGGACGAGGGAGGCGGAAAACCAGGCGGGCGTGCTGCACGTTGGGCGTGTA
TACAAAGAGCGCCTGGGCCTCTCCCCAAAGACCATCATTGGGTACCAGGCCCATGCAGACACAGCCACC
AAGAGCAACTCCCTAGCCAAGAACAAGTTTGTGGTGTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_001099408
Insert Size 729 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001099408.1
RefSeq Size 1974 bp
RefSeq ORF 729 bp
Locus ID 253314
UniProt ID A6NMX2
Protein Pathways Insulin signaling pathway, mTOR signaling pathway
MW 27.6 kDa
Gene Summary Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structure.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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