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PTCH1 (NM_001083603) Human Untagged Clone
CAT＃: SC316084
PTCH1 (untagged)-Human patched 1 (PTCH1), transcript variant 1a'
CN¥ 27,840.00
Product images
CN¥ 1,999.00
CN¥ 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BCNS; NBCCS; PTC; PTC1; PTCH |
| Vector | pCMV6 series |
| Sequence Data |
>NCBI ORF sequence for NM_001083603, the custom clone sequence may differ by one or more nucleotides
ATGGAACTGCTTAATAGAAACAGGCTTGTAATTGTGAGTCCGCGCTGCACTCCGCCGAAA GCCTCCGGCGGCCCAGCGCGCCGGGGTTTTTACACTTTCCGTTCCTTTTGTAAAGACGGA GGAGGAGGAGAAGAAGAAGAAGAAAACGGAGGAGAAGAAAAAGACGACAGGGGAGACAAA GAGACCCGCAGCGACAAGGGGAAGGCTACTGGCCGGAAAGCGCCGCTGTGGCTGAGAGCG AAGTTTCAGAGACTCTTATTTAAACTGGGTTGTTACATTCAAAAAAACTGCGGCAAGTTC TTGGTTGTGGGCCTCCTCATATTTGGGGCCTTCGCGGTGGGATTAAAAGCAGCGAACCTC GAGACCAACGTGGAGGAGCTGTGGGTGGAAGTTGGAGGACGAGTAAGTCGTGAATTAAAT TATACTCGCCAGAAGATTGGAGAAGAGGCTATGTTTAATCCTCAACTCATGATACAGACC CCTAAAGAAGAAGGTGCTAATGTCCTGACCACAGAAGCGCTCCTACAACACCTGGACTCG GCACTCCAGGCCAGCCGTGTCCATGTATACATGTACAACAGGCAGTGGAAATTGGAACAT TTGTGTTACAAATCAGGAGAGCTTATCACAGAAACAGGTTACATGGATCAGATAATAGAA TATCTTTACCCTTGTTTGATTATTACACCTTTGGACTGCTTCTGGGAAGGGGCGAAATTA CAGTCTGGGACAGCATACCTCCTAGGTAAACCTCCTTTGCGGTGGACAAACTTCGACCCT TTGGAATTCCTGGAAGAGTTAAAGAAAATAAACTATCAAGTGGACAGCTGGGAGGAAATG CTGAATAAGGCTGAGGTTGGTCATGGTTACATGGACCGCCCCTGCCTCAATCCGGCCGAT CCAGACTGCCCCGCCACAGCCCCCAACAAAAATTCAACCAAACCTCTTGATATGGCCCTT GTTTTGAATGGTGGATGTCATGGCTTATCCAGAAAGTATATGCACTGGCAGGAGGAGTTG ATTGTGGGTGGCACAGTCAAGAACAGCACTGGAAAACTCGTCAGCGCCCATGCCCTGCAG ACCATGTTCCAGTTAATGACTCCCAAGCAAATGTACGAGCACTTCAAGGGGTACGAGTAT GTCTCACACATCAACTGGAACGAGGACAAAGCGGCAGCCATCCTGGAGGCCTGGCAGAGG ACATATGTGGAGGTGGTTCATCAGAGTGTCGCACAGAACTCCACTCAAAAGGTGCTTTCC TTCACCACCACGACCCTGGACGACATCCTGAAATCCTTCTCTGACGTCAGTGTCATCCGC GTGGCCAGCGGCTACTTACTCATGCTCGCCTATGCCTGTCTAACCATGCTGCGCTGGGAC TGCTCCAAGTCCCAGGGTGCCGTGGGGCTGGCTGGCGTCCTGCTGGTTGCACTGTCAGTG GCTGCAGGACTGGGCCTGTGCTCATTGATCGGAATTTCCTTTAACGCTGCAACAACTCAG GTTTTGCCATTTCTCGCTCTTGGTGTTGGTGTGGATGATGTTTTTCTTCTGGCCCACGCC TTCAGTGAAACAGGACAGAATAAAAGAATCCCTTTTGAGGACAGGACCGGGGAGTGCCTG AAGCGCACAGGAGCCAGCGTGGCCCTCACGTCCATCAGCAATGTCACAGCCTTCTTCATG GCCGCGTTAATCCCAATTCCCGCTCTGCGGGCGTTCTCCCTCCAGGCAGCGGTAGTAGTG GTGTTCAATTTTGCCATGGTTCTGCTCATTTTTCCTGCAATTCTCAGCATGGATTTATAT CGACGCGAGGACAGGAGACTGGATATTTTCTGCTGTTTTACAAGCCCCTGCGTCAGCAGA GTGATTCAGGTTGAACCTCAGGCCTACACCGACACACACGACAATACCCGCTACAGCCCC CCACCTCCCTACAGCAGCCACAGCTTTGCCCATGAAACGCAGATTACCATGCAGTCCACT GTCCAGCTCCGCACGGAGTACGACCCCCACACGCACGTGTACTACACCACCGCTGAGCCG CGCTCCGAGATCTCTGTGCAGCCCGTCACCGTGACACAGGACACCCTCAGCTGCCAGAGC CCAGAGAGCACCAGCTCCACAAGGGACCTGCTCTCCCAGTTCTCCGACTCCAGCCTCCAC TGCCTCGAGCCCCCCTGTACGAAGTGGACACTCTCATCTTTTGCTGAGAAGCACTATGCT CCTTTCCTCTTGAAACCAAAAGCCAAGGTAGTGGTGATCTTCCTTTTTCTGGGCTTGCTG GGGGTCAGCCTTTATGGCACCACCCGAGTGAGAGACGGGCTGGACCTTACGGACATTGTA CCTCGGGAAACCAGAGAATATGACTTTATTGCTGCACAATTCAAATACTTTTCTTTCTAC AACATGTATATAGTCACCCAGAAAGCAGACTACCCGAATATCCAGCACTTACTTTACGAC CTACACAGGAGTTTCAGTAACGTGAAGTATGTCATGTTGGAAGAAAACAAACAGCTTCCC AAAATGTGGCTGCACTACTTCAGAGACTGGCTTCAGGGACTTCAGGATGCATTTGACAGT GACTGGGAAACCGGGAAAATCATGCCAAACAATTACAAGAATGGATCAGACGATGGAGTC CTTGCCTACAAACTCCTGGTGCAAACCGGCAGCCGCGATAAGCCCATCGACATCAGCCAG TTGACTAAACAGCGTCTGGTGGATGCAGATGGCATCATTAATCCCAGCGCTTTCTACATC TACCTGACGGCTTGGGTCAGCAACGACCCCGTCGCGTATGCTGCCTCCCAGGCCAACATC CGGCCACACCGACCAGAATGGGTCCACGACAAAGCCGACTACATGCCTGAAACAAGGCTG AGAATCCCGGCAGCAGAGCCCATCGAGTATGCCCAGTTCCCTTTCTACCTCAACGGCTTG CGGGACACCTCAGACTTTGTGGAGGCAATTGAAAAAGTAAGGACCATCTGCAGCAACTAT ACGAGCCTGGGGCTGTCCAGTTACCCCAACGGCTACCCCTTCCTCTTCTGGGAGCAGTAC ATCGGCCTCCGCCACTGGCTGCTGCTGTTCATCAGCGTGGTGTTGGCCTGCACATTCCTC GTGTGCGCTGTCTTCCTTCTGAACCCCTGGACGGCCGGGATCATTGTGATGGTCCTGGCG CTGATGACGGTCGAGCTGTTCGGCATGATGGGCCTCATCGGAATCAAGCTCAGTGCCGTG CCCGTGGTCATCCTGATCGCTTCTGTTGGCATAGGAGTGGAGTTCACCGTTCACGTTGCT TTGGCCTTTCTGACGGCCATCGGCGACAAGAACCGCAGGGCTGTGCTTGCCCTGGAGCAC ATGTTTGCACCCGTCCTGGATGGCGCCGTGTCCACTCTGCTGGGAGTGCTGATGCTGGCG GGATCTGAGTTCGACTTCATTGTCAGGTATTTCTTTGCTGTGCTGGCGATCCTCACCATC CTCGGCGTTCTCAATGGGCTGGTTTTGCTTCCCGTGCTTTTGTCTTTCTTTGGACCATAT CCTGAGGTGTCTCCAGCCAACGGCTTGAACCGCCTGCCCACACCCTCCCCTGAGCCACCC CCCAGCGTGGTCCGCTTCGCCATGCCGCCCGGCCACACGCACAGCGGGTCTGATTCCTCC GACTCGGAGTATAGTTCCCAGACGACAGTGTCAGGCCTCAGCGAGGAGCTTCGGCACTAC GAGGCCCAGCAGGGCGCGGGAGGCCCTGCCCACCAAGTGATCGTGGAAGCCACAGAAAAC CCCGTCTTCGCCCACTCCACTGTGGTCCATCCCGAATCCAGGCATCACCCACCCTCGAAC CCGAGACAGCAGCCCCACCTGGACTCAGGGTCCCTGCCTCCCGGACGGCAAGGCCAGCAG CCCCGCAGGGACCCCCCCAGAGAAGGCTTGTGGCCACCCCCCTACAGACCGCGCAGAGAC GCTTTTGAAATTTCTACTGAAGGGCATTCTGGCCCTAGCAATAGGGCCCGCTGGGGCCCT CGCGGGGCCCGTTCTCACAACCCTCGGAACCCAGCGTCCACTGCCATGGGCAGCTCCGTG CCCGGCTACTGCCAGCCCATCACCACTGTGACGGCTTCTGCCTCCGTGACTGTCGCCGTG CACCCGCCGCCTGTCCCTGGGCCTGGGCGGAACCCCCGAGGGGGACTCTGCCCAGGCTAC CCTGAGACTGACCACGGCCTGTTTGAGGACCCCCACGTGCCTTTCCACGTCCGGTGTGAG AGGAGGGATTCGAAGGTGGAAGTCATTGAGCTGCAGGACGTGGAATGCGAGGAGAGGCCC CGGGGAAGCAGCTCCAAC |
| Restriction Sites | Please inquire |
| ACCN | NM_001083603 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001083603.1, NP_001077072.1 |
| RefSeq Size | 7911 bp |
| RefSeq ORF | 4341 bp |
| Locus ID | 5727 |
| UniProt ID | Q13635 |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Transmembrane |
| Protein Pathways | Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer |
| Gene Summary | This gene encodes a member of the patched family of proteins and a component of the hedgehog signaling pathway. Hedgehog signaling is important in embryonic development and tumorigenesis. The encoded protein is the receptor for the secreted hedgehog ligands, which include sonic hedgehog, indian hedgehog and desert hedgehog. Following binding by one of the hedgehog ligands, the encoded protein is trafficked away from the primary cilium, relieving inhibition of the G-protein-coupled receptor smoothened, which results in activation of downstream signaling. Mutations of this gene have been associated with basal cell nevus syndrome and holoprosencephaly. [provided by RefSeq, Aug 2017] Transcript Variant: This variant (1a') differs in the 5' UTR and has multiple differences in the 5' coding region, compared to variant 1b. These differences result in a protein (isoform L') with a shorter N-terminus and distinct translation initiation site, compared to isoform L. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220617 | PTCH1 (Myc-DDK-tagged)-Human patched 1 (PTCH1), transcript variant 1a' |
CN¥ 14,440.00 |
|
| RC220617L3 | Lenti-ORF clone of PTCH1 (Myc-DDK-tagged)-Human patched 1 (PTCH1), transcript variant 1a' |
CN¥ 16,340.00 |
|
| RC220617L4 | Lenti-ORF clone of PTCH1 (mGFP-tagged)-Human patched 1 (PTCH1), transcript variant 1a' |
CN¥ 16,340.00 |
|
| RG220617 | PTCH1 (tGFP-tagged) - Human patched 1 (PTCH1), transcript variant 1a' |
CN¥ 15,870.00 |
