MYO7B (NM_001080527) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC315902 representing NM_001080527. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCGGGGTTCAGGCTGGGTGACCACGTGTGGCTGGAGCCTCCCTCCACCCACAAGACCGGCGTGGCC ATCGGGGGCATCATCAAAGAGGCAAAGCCAGGCAAAGTCTTGGTTGAAGATGACGAGGGCAAGGAACAC TGGATCCGAGCAGAGGACTTTGGTGTCCTCAGTCCCATGCACCCCAACTCAGTCCAGGGTGTGGACGAC ATGATCCGCCTGGGGGACCTGAACGAGGCAGGCATGGTGCACAACCTCCTGATCCGCTACCAGCAGCAC AAGATCTATACATACACAGGCTCCATCCTGGTGGCCGTCAACCCGTTCCAGGTGCTGCCGCTCTACACC CTGGAGCAGGTACAGCTCTACTACAGCCGCCATATGGGCGAGCTGCCCCCGCATGTCTTTGCCATCGCC AACAACTGCTACTTCAGCATGAAGAGGAACAAGAGGGACCAGTGCTGCATCATCAGCGGCGAGTCTGGG GCTGGCAAGACGGAGACCACCAAGCTCATCCTGCAGTTCCTGGCCACCATCAGTGGCCAGCATTCGTGG ATTGAGCAGCAGGTCCTGGAAGCCAACCCCATCCTGGAGGCCTTTGGAAATGCCAAGACAATCCGCAAC GACAACTCAAGCCGCTTTGGGAAGTACATTGACATCTACTTTAACCCCAGCGGGGTGATCGAGGGCGCG CGCATCGAGCAATTTCTCCTGGAGAAGTCCCGGGTCTGCCGGCAGGCTCCCGAGGAGCGGAACTACCAT ATCTTCTACTGCATGCTCATGGGGGTGAGTGCTGAGGACAAGCAGCTGCTGAGCCTGGGCACGCCCTCC GAGTACCACTACCTGACCATGGGGAACTGCACTTCCTGTGAGGGGCTCAACGACGCCAAGGACTACGCC CACATCCGCTCGGCCATGAAGATCCTCCAGTTCTCCGACTCCGAGAGCTGGGACGTCATCAAGCTGCTG GCTGCCATTCTCCACCTGGGGAATGTGGGGTTCATGGCTTCGGTCTTCGAGAACCTGGACGCCTCAGAC GTGATGGAGACGCCCGCCTTTCCCACCGTGATGAAGTTACTGGAGGTGCAGCACCAGGAGCTCCGGGAC TGTCTGATCAAGCACACCATCCTCATCCGAGGGGAATTTGTCACCAGGTCCCTGAACATTGCCCAGGCT GCTGACCGGAGGGACGCCTTTGTCAAGGGCATCTATGGGCACCTCTTCCTGTGGATTGTCAAGAAGATC AATGCCGCCATCTTCACACCACCAGCCCAGGACCCCAAAAATGTGCGGAGGGCCATCGGCCTCCTGGAC ATATTTGGCTTTGAAAATTTCGAGAACAATAGCTTCGAGCAGCTCTGCATCAACTTCGCCAACGAGCAC CTGCAGCAGTTCTTTGTGCAGCACGTGTTCACCATGGAGCAAGAGGAGTACCGCTCGGAGAACATCTCC TGGGACTATATCCACTACACCGACAATCGGCCCACCCTGGACCTGCTGGCCCTCAAGCCCATGAGCATC ATCTCCCTCCTGGACGAAGAAAGCCGCTTCCCGCAGGGGACAGATCTCACCATGCTGCAAAAGCTGAAC AGCGTCCATGCCAACAACAAGGCCTTCCTACAGCCCAAGAACATCCACGATGCCAGATTTGGCATTGCC CATTTTGCCGGCGAGGTGTACTACCAAGCAGAAGGCTTCCTGGAGAAGAACCGAGACGTGCTGAGCACA GATATCCTCACCCTGGTTTACTCCTCCAAAAACAAGTTTCTGAGGGAGATATTCAACTTGGAGTTAGCA GAGACCAAGCTGGGCCATGGGACCATCCGCCAGGCAAAGGCAGGAAACCATCTCTTCAAGTCTGCAGAC TCAAATAAACGGCCCTCCACCTTAGGAAGCCAGTTCAAACAGTCTCTGGACCAGCTGATGAAAATCCTG ACCAACTGCCAGCCTTACTTCATCCGCTGCATCAAACCTAATGAGTACAAGAAGCCGCTGCTGTTCGAC CGGGAGCTGTGCCTGCGGCAGCTGCGATACTCGGGCATGATGGAGACCGTGCACATCCGCAAGTCGGGC TTCCCCATCCGCTACACGTTCGAGGAGTTCTCGCAGAGGTTCGGCGTGTTGCTGCCCAACGCCATGCGG ATGCAGCTGCAAGGCAAGCTCCGCCAGATGACCCTGGGCATCACTGACGTGTGGCTGCGGACAGACAAA GACTGGAAAGCGGGGAAGACAAAAATTTTCCTGAGGGATCATCAGGACACTCTGCTGGAGGTACAGAGA AGCCAGGTGCTAGACAGAGCGGCGCTCAGCATCCAGAAAGTCCTTCGGGGCTACAGATACAGGAAGGAG TTCCTGAGGCAGAGGCGGGCAGCTGTGACCCTGCAGGCCTGGTGGAGAGGCTACTGCAACAGGAGGAAT TTCAAGCTGATCCTCGTGGGCTTTGAGCGCCTGCAGGCTATTGCCCGGAGCCAGCCGCTGGCGAGGCAG TACCAGGCCATGCGGCAGAGGACAGTCCAGCTGCAGGCCCTGTGCAGGGGATACCTGGTGCGCCAGCAA GTCCAGGCCAAGAGGAGGGCAGTGGTGGTCATTCAGGCCCATGCCAGGGGCATGGCTGCCCGGCGCAAC TTCCAGCAAAGGAAGGCCAATGCGCCGCTGGTCATCCCGGCCGAGGGGCAGAAAAGCCAAGGCGCTCTC CCTGCCAAGAAGCGCAGATCCATCTACGACACCGTCACTGACACGGAGATGGTGGAGAAGGTGTTCGGC TTCCTCCCTGCCATGATTGGGGGCCAGGAGGGCCAGGCCTCGCCGCACTTTGAGGATCTGGAATCGAAG ACCCAGAAGCTGCTTGAGGTTGACCTGGACACAGTCCCCATGGCGGAGGAGCCTGAGGAGGATGTGGAT GGCCTGGCCGAGTACACCTTCCCCAAGTTTGCTGTGACTTACTTCCAGAAATCAGCCAGCCACACACAC ATCCGGCGGCCCCTCCGATACCCGTTGCTTTACCACGAAGATGACACTGACTGCTTGGCCGCCCTGGTC ATATGGAACGTCATCCTGAGGTTCATGGGTGATCTCCCAGAGCCAGTGCTGTATGCCAGGAGCAGCCAG CAGGGCAGCTCAGTGATGCGGCAGATCCATGACACGCTGGGCAGGGAGCACGGTGCCCAGGTTCCACAG CACAGTAGATCTGCACAGGTGGCCAGCCAGCTGAACATTGGAGAGGAGGCATTGGAGCCTGATGGCCTT GGTGCAGACCGGCCCATGTCCAACCTGGAGAAGGTGCACTTCATCGTGGGCTACGCCATCCTGCGGCCC AGCCTCAGGGATGAGATTTACTGCCAGATCTGCAAGCAGCTCTCGGAGAACTTCAAAACAAGCAGCCTG GCCCGGGGCTGGATCCTGCTCAGCCTCTGCCTCGGCTGCTTCCCACCCTCAGAGAGGTTCATGAAGTAT CTACTGAACTTCATCGGCCAAGGGCCGGCGACCTACGGCCCCTTCTGTGCCGAGCGCCTGAGACGCACC TATGCCAATGGGGTGCGTGCGGAGCCCCCCACCTGGCTGGAGCTGCAGGCTGTCAAGTCCAAGAAGCAC ATCCCCATCCAAGTCATCTTGGCCACTGGAGAGAGCCTAACCGTCCCCGTGGACTCAGCCTCCACATCT CGGGAAATGTGCATGCACATCGCTCACAAGCAGGGCCTCAGCGACCACCTGGGCTTCTCCCTCCAGGTC GCCGTGTACGACAAGTTCTGGTCCCTGGGCAGCGGGCGCGACCACATGATGGATGCCATCGCCCGGTGT GAGCAGATGGCCCAGGAGAGGGGCGAGAGCCAGCGCCAGTCACCCTGGCGCATCTACTTCCGGAAGGAA TTCTTCACCCCCTGGCACGACTCCCGGGAGGACCCTGTCAGCACCGAGCTTATTTACCGCCAAGTCCTC CGAGGAGTCTGGTCTGGCGAGTACAGCTTCGAGAAGGAGGAAGAGCTGGTTGAGCTGCTGGCCCGGCAC TGCTACGTGCAGCTCGGCGCCTCAGCAGAGAGCAAGGCTGTCCAGGAGCTGCTGCCCAGCTGCATCCCC CACAAGCTGTACAGGACCAAGCCCCCAGACAGGTGGGCGAGCCTCGTCACTGCCGCCTGCGCCAAGGCC CCATACACTCAGAAGCAAGTCACACCACTGGCCGTGCGAGAGCAGGTGGTGGACGCCGCCCGCCTGCAG TGGCCGCTGCTCTTCTCCCGGCTCTTCGAAGTCATCACACTCTCAGGCCCCCGCCTGCCCAAGACGCAG CTGATCTTGGCTGTTAACTGGAAGGGGCTTTGCTTCCTGGACCAGCAGGAGAAGATGCTGCTGGAACTC TCTTTCCCAGAGGTCATGGGTCTGGCCACCAACAGGGAGGCCCAGGGCGGGCAGAGGCTGCTGCTCTCC ACGATGCATGAGGAGTACGAGTTTGTGTCACCCAGCAGTGTGGCCATCGCTGAGCTGGTGGCCCTGTTC CTGGAGGGCCTGAAGGAGAGGTCCATTTTCGCCATGGCCCTGCAGGACAGGAAGGCCACAGATGACACC ACCCTCCTGGCCTTCAAGAAGGGGGACCTGTTGGTCCTCACAAAGAAGCAGGGGCTGCTGGCCTCTGAG AACTGGACCCTCGGCCAGAACGACAGGACAGGCAAGACGGGGCTGGTGCCCATGGCCTGCCTCTACACC ATCCCCACGGTCACTAAGCCCTCGGCACAGCTGCTGAGCTTGCTTGCCATGTCACCAGAGAAGAGGAAG CTGGCGGCTCAGGAGGGGCAGTTCACAGAGCCACGTCCTGAGGAGCCACCCAAGGAAAAGCTGCACACC CTGGAGGAGTTCTCCTATGAGTTCTTCAGGGCTCCAGAGAAGGACATGGTGAGCATGGCCGTGCTGCCC CTGGCCCGTGCCCGTGGCCACCTGTGGGCCTATTCCTGCGAGCCGCTGCGACAGCCGCTGCTCAAGCGA GTCCACGCCAACGTCGACCTCTGGGACATCGCCTGCCAGATCTTTGTCGCCATCCTCCGGTACATGGGC GACTACCCTTCTCGGCAGGCCTGGCCCACCCTGGAGCTCACCGACCAGATCTTCACACTGGCCCTGCAG CACCCGGCCCTCCAGGACGAGGTCTACTGCCAGATCCTGAAGCAGCTGACGCACAACTCCAACAGGCAC AGCGAAGAGCGGGGCTGGCAGCTGCTGTGGCTGTGCACGGGCCTCTTCCCGCCCAGCAAGGGGCTGCTG CCCCATGCCCAGAAGTTTATAGACACTCGGAGGGGGAAGCTGCTGGCCCCCGACTGCAGCCGCCGAATC CAGAAGGTCCTGAGGACGGGGCCCCGGAAGCAGCCCCCGCACCAGGTGGAGGTGGAGGCCGCAGAGCAG AACGTCTCCCGCATCTGCCACAAGATCTACTTCCCCAATGACACCAGTGAGATGCTGGAGGTGGTTGCC AACACACGGGTGCGGGATGTGTGTGACAGCATTGCCACCAGGCTGCAGCTGGCCTCCTGGGAGGGCTGC AGCCTCTTCATCAAGATTTCAGACAAGGTCATCAGCCAGAAGGAGGGAGACTTCTTCTTTGATTCCTTG AGGGAGGTGTCTGACTGGGTGAAGAAGAACAAGCCCCAGAAAGAAGGGGCCCCCGTGACGCTCCCCTAC CAGGTGTACTTCATGCGGAAATTGTGGCTCAACATATCTCCAGGGAAGGATGTGAATGCAGACACCATA CTCCATTACCACCAGGAGCTGCCCAAGTACCTGCGCGGATTCCACAAGTGTTCGCGGGAGGATGCCATC CACCTGGCGGGCCTCATCTACAAGGCCCAGTTCAACAACGACCGGTCCCAGCTGGCTAGTGTCCCCAAG ATCCTGAGGGAACTGGTGCCTGAGAACCTCACACGCCTGATGTCCTCGGAGGAGTGGAAAAAGAGCATC CTTCTAGCCTATGACAAGCATAAGGACAAGACAGTGGAGGAGGCCAAGGTGGCCTTCCTGAAGTGGATC TGCCGGTGGCCCACCTTCGGATCCGCCTTCTTCGAGGTGAAGCAAACCTCGGAGCCTTCCTACCCGGAC GTCATCCTCATCGCCATCAACCGACATGGGGTTCTGCTCATCCACCCCAAGACCAAGGACCTGCTCACC ACCTATCCCTTCACCAAGATCTCCAGCTGGAGCAGCGGCAGCACCTACTTCCACATGGCGCTGGGGAGC CTGGGCCGTGGCAGCCGCCTGCTGTGCGAGACCTCCCTGGGCTATAAGATGGATGACCTGCTGACCTCA TATGTGCAGCAGCTCCTGAGTGCCATGAACAAGCAGCGGGGCTCCAAGGCCCCAGCCCTGGCCAGCACC TAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001080527
Insert Size	6351 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001080527.1
RefSeq Size	6715 bp
RefSeq ORF	6351 bp
Locus ID	4648
UniProt ID	Q6PIF6
MW	241.6 kDa
Gene Summary	The protein encoded by this gene is found in brush border microvilli of epithelial cells in the intestines and kidneys. The encoded protein is involved in linking protocadherins to the actin cytoskeleton and is essential for proper microvilli function. This protein aids in the accumulation of intermicrovillar adhesion components such as harmonin and ANKS4B, and this accumulation is necessary for normal brush border action. [provided by RefSeq, Jan 2017]

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