MYO5B (NM_001080467) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC315899 representing NM_001080467. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCGGTGGGCGAGCTCTACAGCCAGTGCACAAGGGTCTGGATCCCTGACCCTGATGAGGTATGGCGC TCAGCTGAGTTAACCAAGGACTACAAAGAAGGAGACAAGAGCCTACAGCTCAGACTGGAGGATGAAACG ATTCTGGAATACCCAATTGATGTACAACGCAACCAGCTGCCCTTCTTACGGAATCCAGATATCTTGGTG GGAGAAAATGACCTGACTGCCCTTAGCTATCTTCATGAGCCTGCAGTTTTGCATAATTTGAAGGTCCGT TTCCTGGAGTCCAACCATATCTACACTTACTGTGGTATCGTACTTGTTGCCATTAATCCTTATGAACAG TTGCCAATCTATGGACAAGATGTCATCTATACCTACAGTGGCCAAAACATGGGAGACATGGACCCCCAC ATCTTTGCTGTGGCAGAAGAAGCCTACAAGCAGATGGCCAGAGATGAGAAGAATCAGTCCATCATAGTC AGTGGGGAGTCTGGAGCCGGGAAGACGGTATCAGCCAAGTATGCCATGCGCTATTTCGCCACCGTTGGT GGCTCGGCCAGTGAAACCAACATCGAAGAGAAGGTGCTGGCATCCAGTCCCATCATGGAGGCCATTGGA AATGCCAAGACCACCCGCAATGACAACAGCAGCCGTTTTGGCAAGTACATCCAGATTGGCTTTGACAAA AGGTACCACATCATCGGGGCCAACATGAGGACTTACCTCTTGGAGAAGTCCAGAGTGGTCTTCCAGGCA GATGATGAGAGGAATTACCACATCTTTTACCAGCTCTGTGCTGCTGCCGGTCTTCCAGAATTTAAAGAG CTTGCACTAACAAGTGCAGAGGACTTTTTCTATACATCACAGGGAGGAGACACTTCCATCGAGGGTGTG GACGATGCTGAGGACTTTGAGAAGACTCGACAAGCCTTCACACTCCTCGGAGTGAAAGAGTCCCATCAG ATGAGCATTTTTAAGATAATTGCTTCTATCTTGCACCTTGGAAGTGTGGCGATTCAGGCTGAGCGTGAT GGTGATTCCTGTAGTATATCACCCCAGGATGTATACCTAAGCAACTTCTGCCGACTGCTAGGGGTGGAG CACAGTCAGATGGAGCACTGGCTGTGTCATCGCAAGCTGGTCACCACCTCGGAGACCTACGTCAAGACC ATGTCCCTGCAGCAGGTGATCAATGCGCGCAACGCCCTGGCGAAGCACATCTATGCCCAGTTGTTCGGC TGGATTGTGGAGCACATCAACAAGGCCCTGCACACCTCCCTCAAGCAGCACTCCTTCATCGGGGTCCTG GACATCTATGGGTTTGAGACATTTGAGGTAAACAGCTTTGAGCAGTTCTGTATCAACTATGCAAATGAA AAGCTCCAGCAGCAGTTCAACTCGCATGTTTTCAAACTGGAGCAAGAAGAATACATGAAGGAACAGATC CCTTGGACCCTGATTGATTTTTATGATAACCAACCTTGTATCGACCTCATTGAAGCCAAGCTGGGTATC TTGGACCTGTTGGATGAAGAATGTAAGGTCCCCAAAGGAACTGACCAGAACTGGGCTCAGAAGCTCTAT GACCGGCACTCCAGCAGCCAGCACTTCCAGAAGCCCCGCATGTCCAACACGGCCTTCATCATCGTCCAC TTTGCAGACAAGGTGGAGTACCTCTCTGATGGTTTTCTGGAGAAAAACAGAGACACGGTGTATGAAGAG CAGATCAATATCCTGAAGGCCAGCAAGTTCCCACTAGTGGCTGACTTGTTTCATGATGACAAGGACCCT GTTCCTGCCACCACCCCTGGGAAGGGGTCATCTTCGAAGATCAGCGTCCGTTCTGCCAGACCCCCCATG AAAGTCTCCAACAAGGAGCACAAGAAAACCGTTGGCCACCAGTTCCGTACCTCCCTGCATCTGCTCATG GAGACCCTGAATGCCACGACACCTCACTATGTCCGCTGCATCAAGCCCAACGATGAGAAGCTCCCCTTT CACTTTGACCCAAAGAGAGCAGTGCAGCAACTCAGAGCCTGCGGGGTGTTGGAGACGATTCGAATCAGT GCAGCTGGCTACCCATCCAGGTGGGCCTACCATGACTTTTTCAACCGGTATCGGGTGCTGGTCAAGAAG AGAGAGCTCGCCAACACAGACAAAAAGGCCATCTGCAGGTCTGTCCTGGAGAACCTCATCAAGGACCCC GACAAGTTCCAGTTTGGCCGCACCAAGATCTTCTTTCGAGCAGGCCAGGTGGCCTACCTGGAGAAGCTG CGGGCTGACAAGTTCCGGACAGCCACCATCATGATCCAGAAAACTGTCCGGGGATGGCTGCAGAAGGTG AAATATCACAGGCTGAAGGGGGCTACCTTAACCCTGCAGAGGTACTGCCGGGGACACCTGGCCCGCAGG CTGGCTGAGCACCTGCGGAGGATCAGAGCGGCTGTGGTGCTCCAGAAACATTACCGCATGCAGAGGGCC CGCCAGGCCTACCAGAGGGTCCGCAGAGCTGCCGTTGTTATCCAGGCCTTCACCCGGGCCATGTTTGTG CGGAGAACCTACCGCCAGGTCCTCATGGAGCACAAGGCCACCACCATCCAGAAGCACGTGCGGGGCTGG ATGGCACGCAGGCACTTCCAGCGGCTGCGGGATGCAGCCATTGTCATCCAGTGTGCCTTCCGGATGCTC AAGGCCAGGCGGGAGCTGAAGGCCCTCAGGATTGAGGCCCGCTCAGCAGAGCATCTGAAACGTCTCAAC GTGGGCATGGAGAACAAGGTGGTCCAGCTGCAGCGGAAGATCGATGAGCAGAACAAAGAGTTCAAGACA CTTTCAGAGCAGTTGTCCGTGACCACCTCAACATACACCATGGAGGTAGAGCGGCTGAAGAAGGAGCTG GTGCACTACCAGCAGAGCCCAGGTGAGGACACCAGCCTCAGGCTGCAGGAGGAGGTGGAGAGCCTGCGC ACAGAGCTGCAGAGGGCCCACTCGGAGCGCAAGATCTTGGAGGACGCCCACAGCAGGGAGAAAGATGAG CTGAGGAAGCGAGTTGCAGACCTGGAGCAAGAAAATGCTCTCTTGAAAGATGAGAAAGAACAGCTCAAC AACCAAATCCTGTGCCAGTCTAAAGATGAATTTGCCCAGAACTCTGTGAAGGAAAATCTCATGAAGAAA GAACTGGAGGAGGAGCGATCCCGGTACCAGAACCTTGTGAAGGAATATTCACAGTTGGAGCAGAGATAC GACAACCTTCGGGATGAAATGACCATCATAAAGCAAACTCCAGGTCATAGGCGGAACCCATCAAACCAA AGTAGCTTAGAATCTGACTCCAATTACCCCTCCATCTCCACATCTGAGATCGGAGACACTGAGGATGCC CTCCAGCAGGTGGAGGAAATTGGCCTGGAGAAGGCAGCCATGGACATGACGGTCTTCCTGAAGCTGCAG AAGAGAGTACGGGAGCTGGAGCAGGAGAGGAAAAAGCTGCAAGTGCAGCTGGAGAAGAGAGAACAGCAG GACAGCAAGAAAGTCCAGGCGGAACCACCACAGACTGACATAGATTTGGACCCGAATGCAGATCTGGCC TACAATAGTCTGAAGAGGCAAGAGCTGGAGTCAGAGAACAAAAAGCTGAAGAATGACCTGAATGAGCTG AGGAAAGCCGTGGCCGACCAAGCCACGCAGAATAACTCCAGCCACGGCTCCCCAGATAGCTACAGCCTC CTGCTGAACCAGCTCAAGCTGGCCCACGAGGAGCTCGAGGTGCGCAAGGAGGAGGTGCTCATCCTCAGG ACCCAGATCGTGAGCGCCGACCAGCGGCGACTCGCCGGCAGGAACGCGGAGCCGAACATTAATGCCAGA TCAAGTTGGCCTAACAGTGAAAAGCATGTTGACCAGGAGGATGCCATTGAGGCCTATCACGGGGTCTGC CAGACAAACAGCAAGACTGAGGATTGGGGATATTTAAATGAAGATGGAGAACTCGGCTTGGCCTACCAA GGCCTAAAGCAAGTTGCCAGGCTGCTGGAGGCTCAGCTGCAGGCCCAGAGCCTGGAGCATGAGGAGGAG GTGGAGCATCTCAAGGCTCAGCTCGAGGCCCTGAAGGAGGAGATGGACAAACAGCAGCAGACCTTCTGC CAGACGCTACTGCTCTCCCCAGAGGCCCAGGTGGAATTCGGCGTTCAGCAGGAAATATCCCGGCTGACC AACGAGAATCTGGACCTTAAAGAACTGGTAGAAAAGCTGGAAAAGAATGAGAGGAAGCTCAAAAAGCAA CTGAAGATTTACATGAAGAAAGCCCAGGACCTAGAAGCTGCCCAGGCATTGGCCCAGAGTGAGAGGAAG CGCCATGAGCTCAACAGGCAGGTCACGGTCCAGCGGAAAGAGAAGGATTTCCAGGGCATGCTGGAGTAC CACAAAGAGGACGAGGCCCTCCTCATCCGGAACCTGGTGACAGACTTGAAGCCCCAGATGCTGTCGGGC ACAGTGCCCTGTCTCCCCGCCTACATCCTCTACATGTGCATCCGGCACGCGGACTACACCAACGACGAT CTCAAGGTGCACTCCCTGCTGACCTCCACCATCAACGGCATTAAGAAAGTCCTGAAAAAGCACAATGAT GACTTTGAGATGACGTCATTCTGGTTATCCAACACCTGCCGCCTTCTTCACTGTCTGAAGCAGTACAGC GGGGATGAGGGCTTCATGACTCAGAACACTGCAAAGCAGAATGAACACTGTCTTAAGAATTTTGACCTC ACCGAATACCGTCAGGTGCTGAGTGACCTTTCCATTCAGATCTACCAGCAGCTCATTAAAATTGCCGAG GGCGTGTTACAGCCGATGATAGTTTCTGCCATGTTGGAAAATGAGAGCATTCAGGGTCTATCTGGTGTG AAGCCCACCGGCTACCGGAAGCGCTCCTCCAGCATGGCAGATGGGGATAACTCATACTGCCTGGAAGCT ATCATCCGCCAGATGAATGCCTTTCATACAGTCATGTGTGACCAGGGCTTGGACCCTGAGATCATCCTG CAGGTATTCAAACAGCTCTTCTACATGATCAACGCAGTGACTCTTAACAACCTGCTCTTGCGGAAGGAC GTCTGCTCTTGGAGCACAGGCATGCAACTCAGGTACAATATAAGTCAGCTTGAGGAGTGGCTTCGGGGA AGAAACCTTCACCAGAGTGGAGCAGTTCAGACCATGGAACCTCTGATCCAAGCAGCCCAGCTCCTGCAA TTAAAGAAGAAAACCCAGGAGGACGCAGAGGCTATCTGCTCCCTGTGTACCTCCCTCAGCACCCAGCAG ATTGTCAAAATTTTAAACCTTTATACTCCCCTGAATGAATTTGAAGAACGGGTAACAGTGGCCTTTATA CGAACAATCCAGGCACAACTACAAGAGCGGAATGACCCTCAGCAACTGCTATTAGATGCCAAGCACATG TTTCCTGTTTTGTTTCCATTTAATCCATCTTCTCTAACCATGGACTCAATCCACATCCCAGCGTGTCTC AATCTGGAATTCCTCAATGAAGTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001080467
Insert Size	5547 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001080467.2
RefSeq Size	9520 bp
RefSeq ORF	5547 bp
Locus ID	4645
UniProt ID	Q9ULV0
MW	213.7 kDa
Gene Summary	The protein encoded by this gene, together with other proteins, may be involved in plasma membrane recycling. Mutations in this gene are associated with microvillous inclusion disease. [provided by RefSeq, Sep 2009]

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