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KIAA1543 (CAMSAP3) (NM_001080429) Human Untagged Clone
CAT＃: SC315878
CAMSAP3 (untagged)-Human KIAA1543 (KIAA1543), transcript variant 1
CN¥ 24,700.00
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Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | KIAA1543; NEZHA; PPP1R80 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC315878 representing NM_001080429.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGTGGAGGCGGCGCCCCCCGGGCCCGGGCCGCTGCGGAGGACCTTTCTAGTGCCCGAGATCAAGTCG CTGGACCAGTACGATTTCTCGCGGGCCAAGGCGGCGGCCAGCCTGGCGTGGGTGCTGCGGGCCGCGTTC GGGGGCGCAGAGCACGTGCCCCCGGAGCTGTGGGAGCCCTTCTATACCGACCAGTACGCGCAGGAGCAT GTGAAGCCCCCGGTGACACGGCTGCTGCTCTCAGCCGAGCTCTACTGCAGAGCCTGGCGCCAGGCACTG CCACAGCTTGAAACACCCCCCAACCCCTCTGCACTGCTGGCCCTGCTGGCGCGGAGGGGCACAGTGCCT GCTTTGCCCGAGCGCCCGGTGCGCGAGGCCGACCTGAGGCACCAGCCCATTCTCATGGGAGCCCACCTA GCTGTCATTGATGCCCTCATGGCTGCCTTTGCCTTCGAGTGGACAAAGACCCTGCCAGGTCCCTTGGCC CTGACCAGCTTGGAGCACAAGCTGCTTTTCTGGGTGGACACGACCGTCCGGCGGCTGCAGGAGAAGACC GAGCAGGAAGCGGCCCAGCGAGCCTCTCCAGCAGCCCCTGCAGACGGGGCGGCCCCGGCGCAGCCCTCG TGCCCTACGCGCTGGTACTGGAAGCTGGTTCCTCACGCAATTGCCTTCTGTTTGAAGGAGTCGGGGAGC AAACCCCCCATGATCCGATACCGCAAGGACCGTGTGGTGGCGCGACGTGCCCCCTGCTTCCCGACGGTG ACCAGCCTCCAGGACCTGGCCAGTGGGGCCGCGCTGGCCGCCACCATCCACTGCTATTGTCCCCAGCTG CTTCGACTTGAGGAGGTGTGCTTGAAGGACCCCATGTCTGTGGCGGACAGCCTGTACAACCTCCAGCTC GTGCAGGATTTCTGTGCCTCTCGCCTTCCTCGTGGCTGCCCCCTGTCCCTTGAGGACTTGCTGTACGTC CCACCGCCACTCAAGGTCAACTTGGTGGTGATGCTGGCCGAGTTGTTCATGTGTTTTGAGGTGCTCAAG CCCGACTTTGTGCAAGTGAAGGACTTGCCCGATGGTCACGCTGCCTCCCCCCGGGGCACTGAGGCCTCC CCACCTCAGAACAACAGCGGCAGTAGTTCTCCTGTCTTCACCTTCCGCCACCCGCTTCTGTCATCTGGT GGCCCCCAGTCCCCACTCCGAGGATCCACAGGCTCCCTGAAGTCTTCCCCGTCCATGTCCCATATGGAG GCCCTGGGCAAGGCCTGGAACCGGCAGCTCAGCCGTCCCCTCTCCCAGGCTGTGTCATTCAGCACCCCC TTTGGCCTGGACAGCGACGTGGATGTCGTCATGGGAGACCCTGTGCTCCTCCGCTCTGTGAGCTCGGAC AGCCTGGGCCCCCCGCGTCCCGCGCCGGCCAGGACCCCCACCCAGCCACCCCCGGAGCCTGGTGACCTG CCCACCATCGAGGAAGCTCTGCAGATCATCCACAGTGCCGAGCCCCGGCTCCTCCCAGATGGGGCGGCC GACGGCAGCTTCTACCTCCACTCCCCTGAGGGGCCCTCCAAGCCATCCCTGGCCTCCCCCTACCTGCCC GAGGGGACCTCCAAACCACTGTCCGACAGGCCCACCAAAGCACCAGTGTACATGCCACACCCCGAGACC CCCTCGAAACCATCTCCCTGTCTGGTGGGGGAGGCATCGAAACCGCCAGCCCCATCCGAGGGGTCCCCG AAGGCGGTGGCTTCGTCCCCAGCAGCCACCAACTCCGAGGTGAAAATGACCAGCTTTGCAGAACGCAAG AAACAGCTGGTGAAGGCAGAGGCTGAGGCCGGAGCGGGGTCCCCCACGTCCACTCCGGCCCCGCCGGAG GCCCTGAGCTCGGAGATGAGTGAGCTCAGCGCCCGGCTGGAGGAGAAACGCAGAGCCATCGAGGCTCAG AAGCGACGGATTGAGGCCATATTCGCCAAGCACCGCCAGCGGCTGGGCAAAAGCGCCTTCCTGCAGGTG CAGCCGCGGGAAGCCTCTGGGGAGGCGGAAGCAGAGGCGGAGGAGGCCGATTCCGGTCCAGTCCCTGGT GGGGAGCGGCCCGCAGGCGAGGGCCAGGGTGAGCCAACCTCACGGCCCAAGGCAGTGACCTTCTCGCCA GACCTGGGCCCGGTGCCCCACGAGGGGCTGGGGGAATACAATCGAGCGGTCAGCAAGCTGAGTGCCGCC TTGAGCTCGCTGCAGCGGGACATGCAGAGGCTCACGGACCAGCAGCAGCGGCTCCTGGCCCCGCCCGAG GCCCCCGGATCCGCCCCACCACCTGCTGCGTGGGTCATCCCTGGCCCCACGACGGGGCCCAAAGCTGCA TCCCCCAGCCCCGCCCGGCGAGTCCCGGCCACCCGGCGCAGCCCTGGGCCCGGGCCCAGCCAGTCACCC CGCAGCCCGAAACACACGCGGCCAGCGGAGCTGCGGCTGGCACCCTTGACCAGGGTGCTTACGCCACCC CACGACGTAGACAGCCTCCCCCACCTGCGCAAGTTCTCGCCGAGCCAGGTGCCCGTGCAGACGCGCTCT TCCATCCTCCTGGCGGAGGAGACGCCCCCCGAGGAGCCAGCCGCCCGGCCGGGCCTCATCGAGATCCCG CTGGGCAGCCTGGCAGATCCCGCCGCCGAGGACGAGGGAGACGGGAGCCCCGCTGGTGCTGAGGATTCC TTGGAGGAGGAGGCGTCTTCGGAGGGGGAGCCCCGGGTGGGGCTGGGGTTCTTCTACAAGGATGAAGAC AAGCCTGAGGACGAGATGGCCCAAAAGCGGGCCAGCCTGCTGGAGCGGCAGCAGCGGCGAGCAGAGGAG GCGCGGCGGCGCAAGCAGTGGCAGGAGGTGGAGAAGGAACAGCGGAGGGAGGAGGCCGCGAGGCTGGCC CAAGAGGAGGCCCCGGGCCCAGCCCCGCTTGTGTCCGCAGTCCCGATGGCGACTCCAGCCCCTGCTGCC CGGGCTCCAGCCGAGGAGGAGGTGGGCCCCCGGAAGGGGGACTTCACGCGGCAGGAGTACGAGCGCCGG GCCCAGCTGAAGCTGATGGACGACCTCGATAAGGTGCTGCGGCCCCGGGCTGCGGGGTCCGGGGGTCCA GGTCGGGGCGGGCGGAGGGCCACCCGGCCTCGCTCGGGTTGCTGTGACGACTCAGCCCTGGCACGAAGC CCAGCCCGCGGCCTGCTGGGCTCTCGGCTGAGCAAAATCTATTCCCAGTCCACCCTGTCACTGTCCACT GTGGCCAACGAGGCCCACAATAACCTCGGGGTGAAGAGGCCCACGTCTCGGGCTCCCTCCCCGTCAGGT CTCATGTCCCCAAGCCGCCTGCCTGGAAGCCGCGAACGGGACTGGGAAAATGGCAGCAATGCCTCCTCC CCAGCGTCAGTGCCCGAGTACACAGGTCCACGGCTGTACAAAGAACCCAGCGCCAAGTCCAACAAGTTC ATCATCCACAATGCCCTATCACACTGCTGCCTGGCGGGCAAGGTGAACGAACCGCAGAAGAATCGCATT CTGGAGGAAATTGAGAAAAGCAAGGCCAACCACTTCCTGATCCTCTTTCGCGACTCGAGCTGCCAGTTC CGGGCGCTCTACACGCTGTCGGGGGAGACAGAGGAGCTGTCGCGGCTGGCAGGGTATGGGCCCCGGACC GTCACGCCCGCCATGGTGGAAGGCATCTACAAGTACAACTCGGACCGCAAGCGCTTCACCCAGATCCCC GCCAAGACCATGTCCATGAGCGTCGATGCCTTCACCATCCAGGGACACCTCTGGCAGGGCAAGAAACCC ACCACTCCCAAGAAGGGCGGCGGCACCCCCAAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001080429 |
| Insert Size | 3831 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001080429.2 |
| RefSeq Size | 4201 bp |
| RefSeq ORF | 3831 bp |
| Locus ID | 57662 |
| UniProt ID | Q9P1Y5 |
| MW | 137.9 kDa |
| Gene Summary | Key microtubule-organizing protein that specifically binds the minus-end of non-centrosomal microtubules and regulates their dynamics and organization (PubMed:19041755, PubMed:23169647). Specifically recognizes growing microtubule minus-ends and autonomously decorates and stabilizes microtubule lattice formed by microtubule minus-end polymerization (PubMed:24486153). Acts on free microtubule minus-ends that are not capped by microtubule-nucleating proteins or other factors and protects microtubule minus-ends from depolymerization (PubMed:24486153). In addition, it also reduces the velocity of microtubule polymerization (PubMed:24486153). Required for the biogenesis and the maintenance of zonula adherens by anchoring the minus-end of microtubules to zonula adherens and by recruiting the kinesin KIFC3 to those junctional sites (PubMed:19041755). Required for orienting the apical-to-basal polarity of microtubules in epithelial cells: acts by tethering non-centrosomal microtubules to the apical cortex, leading to their longitudinal orientation (PubMed:27802168, PubMed:26715742). Plays a key role in early embryos, which lack centrosomes: accumulates at the microtubule bridges that connect pairs of cells and enables the formation of a non-centrosomal microtubule-organizing center that directs intracellular transport in the early embryo (By similarity). Couples non-centrosomal microtubules with actin: interaction with MACF1 at the minus ends of non-centrosomal microtubules, tethers the microtubules to actin filaments, regulating focal adhesion size and cell migration (PubMed:27693509). Plays a key role in the generation of non-centrosomal microtubules by accumulating in the pericentrosomal region and cooperating with KATNA1 to release non-centrosomal microtubules from the centrosome (PubMed:28386021). Through the microtubule cytoskeleton, also regulates the organization of cellular organelles including the Golgi and the early endosomes (PubMed:28089391). Through interaction with AKAP9, involved in translocation of Golgi vesicles in epithelial cells, where microtubules are mainly non-centrosomal (PubMed:28089391).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longer isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216127 | CAMSAP3 (Myc-DDK-tagged)-Human KIAA1543 (KIAA1543), transcript variant 1 |
CN¥ 8,888.00 |
|
| RC216127L1 | Lenti ORF clone of Human KIAA1543 (KIAA1543), transcript variant 1, Myc-DDK-tagged |
CN¥ 11,288.00 |
|
| RC216127L2 | Lenti ORF clone of Human KIAA1543 (KIAA1543), transcript variant 1, mGFP tagged |
CN¥ 11,500.00 |
|
| RC216127L3 | Lenti ORF clone of Human KIAA1543 (KIAA1543), transcript variant 1, Myc-DDK-tagged |
CN¥ 11,500.00 |
|
| RC216127L4 | Lenti ORF clone of Human KIAA1543 (KIAA1543), transcript variant 1, mGFP tagged |
CN¥ 11,500.00 |
|
| RG216127 | CAMSAP3 (tGFP-tagged) - Human KIAA1543 (KIAA1543), transcript variant 1 |
CN¥ 10,550.00 |
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