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UBE4A (NM_004788) Human Untagged Clone
CAT＃: SC315144
UBE4A (untagged)-Human ubiquitination factor E4A (UBE4A), transcript variant 1
CN¥ 7,480.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | E4; UBOX2; UFD2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC315144 representing NM_004788.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACAGACCAGGAGAATAACAACAACATCTCAAGTAACCCCTTTGCTGCTCTTTTTGGCTCCCTGGCT GATGCCAAACAGTTTGCGGCAATCCAAAAAGAGCAGCTGAAGCAACAATCTGATGAACTCCCAGCTAGC CCAGATGACTCGGATAATAGCGTGTCAGAGAGCCTGGATGAATTCGATTACTCTGTGGCTGAGATTAGC CGCTCATTCCGATCACAGCAGGAAATATGTGAGCAACTCAACATCAATCACATGATCCAAAGGATCTTC CTTATTACTCTGGACAACAGTGATCCCAGCTTGAAAAGCGGGAATGGCATCCCTAGCCGTTGTGTGTAT TTGGAAGAAATGGCAGTAGAGCTAGAAGATCAAGACTGGCTTGATATGAGCAATGTTGAGCAGGCCCTC TTCGCTCGCTTATTACTTCAAGATCCAGGCAACCACTTAATTAACATGACTTCTTCTACAACGCTAAAT CTCTCTGCTGATCGAGATGCAGGAGAGAGGCACATTTTTTGTTACCTTTACTCCTGCTTCCAGAGAGCC AAGGAAGAGATTACCAAAGTTCCAGAGAACCTGCTACCCTTTGCAGTGCAGTGCAGAAACCTCACTGTG TCCAATACCCGAACAGTTCTTCTCACCCCAGAGATCTATGTTGACCAAAACATCCATGAGCAACTGGTA GATTTGATGTTAGAAGCCATCCAGGGAGCCCGTGAGTACATGAACAAGATCTATTTTGAAGATGTAACT GAGTTTCTGGAAGAGGTCATTGAAGCCTTGATATTGGATGAGGAAGTTAGAACATTTCCAGAAGTCATG ATTCCAGTGTTTGATATTTTATTGGGCCGAATAAAAGATCTAGAGCTCTGTCAGATCCTTTTGTATGCA TATCTGGATATTCTTCTCTATTTCACTAGGCAAAAAGATATGGCAAAGGTTTTTGTAGAATACATTCAG CCCAAGGACCCTACCAATGGGCAAATGTACCAGAAGACCTTGCTGGGAGTAATTCTGAGTATCTCCTGC TTATTAAAGACTCCGGGTGTTGTAGAAAATCATGGCTACTTTTTGAATCCATCTCGTTCCAGCCCCCAG GAGATCAAAGTACAGGAGGCCAACATCCATCAGTTCATGGCTCAGTTCCACGAAAAGATCTACCAGATG CTGAAGAACTTACTCCAGCTCTCTCCAGAAACCAAACACTGTATCTTGTCCTGGCTTGGAAACTGTTTG CATGCAAATGCAGGCCGCACCAAGATTTGGGCCAATCAGATGCCAGAAATCTTTTTCCAAATGTATGCC TCAGATGCTTTCTTTCTGAATCTGGGTGCTGCTCTCCTGAAGCTATGCCAGCCATTTTGCAAACCCAGA TCCTCTCGGCTCCTCACCTTTAATCCCACATACTGTGCCCTCAAGGAGTTGAATGATGAAGAACGAAAA ATTAAAAATGTACACATGAGAGGTTTGGACAAAGAAACCTGTTTGATCCCAGCTGTGCAGGAGCCGAAG TTTCCACAGAACTACAACCTTGTAACAGAGAACCTTGCTCTGACAGAGTACACCTTGTACTTGGGATTT CACAGGTTGCATGATCAGATGGTAAAAATCAACCAAAATCTGCATCGGCTGCAGGTTGCCTGGCGGGAT GCTCAGCAAAGTTCTAGCCCTGCTGCTGACAATCTTCGTGAGCAGTTTGAACGACTGATGACCATCTAT CTTTCTACCAAGACTGCCATGACAGAGCCACAAATGCTACAAAACTGCCTAAACTTGCAGGTGTCCATG GCTGTTCTACTGGTTCAACTGGCCATAGGCAATGAGGGCTCACAGCCAATAGAGCTAACCTTTCCTTTG CCAGATGGCTACAGCTCTTTGGCTTATGTGCCAGAATTTTTTGCAGATAACCTGGGTGATTTTCTCATT TTTCTCCGCCGCTTTGCCGATGACATTTTGGAGACATCAGCAGATTCCCTGGAGCATGTCCTTCACTTT ATCACCATTTTCACTGGAAGCATAGAAAGAATGAAGAATCCCCACCTGAGGGCCAAACTAGCAGAGGTG TTGGAAGCAGTGATGCCCCACCTGGATCAGACCCCAAATCCCTTGGTATCCAGTGTGTTCCACCGGAAA CGTGTGTTCTGCAACTTTCAGTATGCACCCCAACTTGCAGAGGCTCTAATCAAGGTTTTTGTGGACATC GAATTTACAGGAGACCCCCATCAATTTGAACAGAAGTTTAATTACCGCCGTCCCATGTATCCTATCCTA AGATACATGTGGGGGACAGATACCTATCGGGAGAGCATTAAGGATTTGGCTGACTATGCCTCTAAGAAT TTAGAAGCCATGAATCCCCCACTTTTCCTCCGCTTTCTTAACCTGCTAATGAATGATGCCATCTTCCTT TTGGATGAAGCCATACAGTATTTGAGCAAGATAAAGATTCAGCAAATTGAGAAGGATCGAGGTGAATGG GATAGTCTGACTCCAGAAGCCCGCCGAGAAAAGGAGGCTGGCCTACAGATGTTTGGACAGCTGGCACGT TTCCATAACATCATGTCCAATGAAACAATCGGTACCCTTGCCTTTCTCACATCAGAGATCAAGTCACTC TTTGTGCATCCCTTCCTGGCTGAGCGCATCATCTCCATGTTGAACTACTTCCTGCAACACCTGGTTGGC CCCAAGATGGGTGCCTTAAAAGTCAAGGACTTCAGCGAATTTGACTTCAAACCCCAGCAGCTTGTATCA GATATCTGCACTATCTACTTAAATCTTGGGGATGAGGAGAATTTCTGTGCCACTGTGCCCAAGGATGGA CGTTCCTATTCCCCAACTCTCTTTGCACAGACAGTTCGAGTCTTGAAGAAAATAAATAAGCCTGGGAAT ATGATTATGGCTTTCAGCAACTTGGCAGAGAGAATCAAGTCTCTTGCAGACCTCCAACAACAGGAAGAG GAAACCTATGCAGATGCCTGTGATGAGTTCCTGGATCCCATTATGAGCACACTGATGTGTGACCCTGTG GTGCTGCCATCTTCCAGAGTCACTGTGGATAGATCCACCATTGCAAGACATTTGCTCAGTGACCAAACA GATCCCTTTAACCGTAGTCCCCTCACCATGGACCAGATCCGGCCAAACACAGAACTAAAAGAAAAAATC CAACGGTGGCTTGCAGAGAGGAAACAACAAAAGGAGCAACTTGAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_004788 |
| Insert Size | 3222 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004788.2 |
| RefSeq Size | 6144 bp |
| RefSeq ORF | 3222 bp |
| Locus ID | 9354 |
| UniProt ID | Q14139 |
| Domains | U-box |
| Protein Families | Druggable Genome |
| Protein Pathways | Ubiquitin mediated proteolysis |
| MW | 123.5 kDa |
| Gene Summary | This gene encodes a member of the U-box ubiquitin ligase family. The encoded protein is involved in multiubiquitin chain assembly and plays a critical role in chromosome condensation and separation through the polyubiquitination of securin. Autoantibodies against the encoded protein may be markers for scleroderma and Crohn's disease. A pseudogene of this gene is located on the long arm of chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2011] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217407 | UBE4A (Myc-DDK-tagged)-Human ubiquitination factor E4A (UBE4A), transcript variant 1 |
CN¥ 7,472.00 |
|
| RC217407L1 | Lenti ORF clone of Human ubiquitination factor E4A (UBE4A), transcript variant 1, Myc-DDK-tagged |
CN¥ 9,872.00 |
|
| RC217407L2 | Lenti ORF clone of Human ubiquitination factor E4A (UBE4A), transcript variant 1, mGFP tagged |
CN¥ 12,640.00 |
|
| RC217407L3 | Lenti ORF clone of Human ubiquitination factor E4A (UBE4A), transcript variant 1, Myc-DDK-tagged |
CN¥ 12,640.00 |
|
| RC217407L4 | Lenti ORF clone of Human ubiquitination factor E4A (UBE4A), transcript variant 1, mGFP tagged |
CN¥ 12,640.00 |
|
| RG217407 | UBE4A (tGFP-tagged) - Human ubiquitination factor E4A (UBE4A), transcript variant 1 |
CN¥ 11,780.00 |
