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Ataxin 1 (ATXN1) (NM_000332) Human Untagged Clone
CAT＃: SC314762
ATXN1 (untagged)-Human ataxin 1 (ATXN1), transcript variant 1
CNY 5,976.00
CNY 7,220.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ATX1; D6S504E; SCA1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_000332 edited
GAAACAGTCACCGTGGAGGGGGGACGGCGAAAAATGAAATCCAACCAAGAGCGGAGCAAC GAATGCCTGCCTCCCAAGAAGCGCGAGATCCCCGCCACCAGCCGGTCCTCCGAGGAGAAG GCCCCTACCCTGCCCAGCGACAACCACCGGGTGGAGGGCACAGCATGGCTCCCGGGCAAC CCTGGTGGCCGGGGCCACGGGGGCGGGAGGCATGGGCCGGCAGGGACCTCGGTGGAGCTT GGTTTACAACAGGGAATAGGTTTACACAAAGCATTGTCCACAGGGCTGGACTACTCCCCG CCCAGCGCTCCCAGGTCTGTCCCCGTGGCCACCACGCTGCCTGCCGCGTACGCCACCCCG CAGCCAGGGACCCCGGTGTCCCCCGTGCAGTACGCTCACCTGCCGCACACCTTCCAGTTC ATTGGGTCCTCCCAATACAGTGGAACCTATGCCAGCTTCATCCCATCACAGCTGATCCCC CCAACCGCCAACCCCGTCACCAGTGCAGTGGCCTCGGCCGCAGGGGCCACCACTCCATCC CAGCGCTCCCAGCTGGAGGCCTATTCCACTCTGCTGGCCAACATGGGCAGTCTGAGCCAG ACGCCGGGACACAAGGCTGAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAT CAGCATCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCACCTCAGCAGG GCTCCGGGGCTCATCACCCCGGGGTCCCCCCCACCAGCCCAGCAGAACCAGTACGTCCAC ATTTCCAGTTCTCCGCAGAACACCGGCCGCACCGCCTCTCCTCCGGCCATCCCCGTCCAC CTCCACCCCCACCAGACGATGATCCCACACACGCTCACCCTGGGGCCCCCCTCCCAGGTC GTCATGCAATACGCCGACTCCGGCAGCCACTTTGTCCCTCGGGAGGCCACCAAGAAAGCT GAGAGCAGCCGGCTGCAGCAGGCCATCCAGGCCAAGGAGGTCCTGAACGGTGAGATGGAG AAGAGCCGGCGGTACGGGGCCCCGTCCTCAGCCGACCTGGGCCTGGGCAAGGCAGGCGGC AAGTCGGTTCCTCACCCGTACGAGTCCAGGCACGTGGTGGTCCACCCGAGCCCCTCAGAC TACAGCAGTCGTGATCCTTCGGGGGTCCGGGCCTCTGTGATGGTCCTGCCCAACAGCAAC ACGCCCGCAGCTGACCTGGAGGTGCAACAGGCCACTCATCGTGAAGCCTCCCCTTCTACC CTCAACGACAAAAGTGGCCTGCATTTAGGGAAGCCTGGCCACCGGTCCTACGCGCTCTCA CCCCACACGGTCATTCAGACCACACACAGTGCTTCAGAGCCACTCCCGGTGGGACTGCCA GCCACGGCCTTCTACGCAGGGACTCAACCCCCTGTCATCGGCTACCTGAGCGGCCAGCAG CAAGCAATCACCTACGCCGGCAGCCTGCCCCAGCACCTGGTGATCCCCGGCACACAGCCC CTGCTCATCCCGGTCGGCAGCACTGACATGGAAGCGTCGGGGGCAGCCCCGGCCATAGTC ACGTCATCCCCCCAGTTTGCTGCAGTGCCTCACACGTTCGTCACCACCGCCCTTCCCAAG AGCGAGAACTTCAACCCTGAGGCCCTGGTCACCCAGGCCGCCTACCCAGCCATGGTGCAG GCCCAGATCCACCTGCCTGTGGTGCAGTCCGTGGCCTCCCCGGCGGCGGCTCCCCCTACG CTGCCTCCCTACTTCATGAAAGGCTCCATCATCCAGTTGGCCAACGGGGAGCTAAAGAAG GTGGAAGACTTAAAAACAGAAGATTTCATCCAGAGTGCAGAGATAAGCAACGACCTGAAG ATCGACTCCAGCACCGTAGAGAGGATTGAAGACAGCCATAGCCCGGGCGTGGCCGTGATA CAGTTCGCCGTCGGGGAGCACCGAGCCCAGGTCAGCGTTGAAGTTTTGGTAGAGTATCCT TTTTTTGTGTTTGGACAGGGCTGGTCATCCTGCTGTCCGGAGAGAACCAGCCAGCTCTTT GATTTGCCGTGTTCCAAACTCTCAGTTGGGGATGTCTGCATCTCGCTTACCCTCAAGAAC CTGAAGAACGGCTCTGTTAAAAAGGGCCAGCCCGTGGATCCCGCCAGCGTCCTGCTGAAG CACTCAAAGGCCGACGGCCTGGCGGGCAGCAGACACAGGTATGCCGAGCAGGAAAACGGA ATCAACCAGGGGAGTGCCCAGATGCTCTCTGAGAATGGCGAACTGAAGTTTCCAGAGAAA ATGGGATTGCCTGCAGCGCCCTTCCTCACCAAAATAGAACCCAGCAAGCCCGCGGCAACG AGGAAGAGGAGGTGGTCGGCGCCAGAGAGCCGCAAACTGGAGAAGTCAGAAGACGAACCA CCTTTGACTCTTCCTAAGCCTTCTCTAATTCCTCAGGAGGTTAAGATTTGCATTGAAGGC CGGTCTAATGTAGGCAAGTAGAGGCAGCGTGGGGGAAAGGAAACGTG |
| Restriction Sites | Please inquire |
| ACCN | NM_000332 |
| Insert Size | 2500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The ORF of this clone has been fully sequenced and found to be a perfect match to NM_000332.2. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000332.2, NP_000323.2 |
| RefSeq Size | 10692 bp |
| RefSeq ORF | 2448 bp |
| Locus ID | 6310 |
| UniProt ID | P54253 |
| Domains | AXH |
| Gene Summary | The autosomal dominant cerebellar ataxias (ADCA) are a heterogeneous group of neurodegenerative disorders characterized by progressive degeneration of the cerebellum, brain stem and spinal cord. Clinically, ADCA has been divided into three groups: ADCA types I-III. ADCAI is genetically heterogeneous, with five genetic loci, designated spinocerebellar ataxia (SCA) 1, 2, 3, 4 and 6, being assigned to five different chromosomes. ADCAII, which always presents with retinal degeneration (SCA7), and ADCAIII often referred to as the `pure' cerebellar syndrome (SCA5), are most likely homogeneous disorders. Several SCA genes have been cloned and shown to contain CAG repeats in their coding regions. ADCA is caused by the expansion of the CAG repeats, producing an elongated polyglutamine tract in the corresponding protein. The expanded repeats are variable in size and unstable, usually increasing in size when transmitted to successive generations. The function of the ataxins is not known. This locus has been mapped to chromosome 6, and it has been determined that the diseased allele contains 40-83 CAG repeats, compared to 6-39 in the normal allele, and is associated with spinocerebellar ataxia type 1 (SCA1). Alternative splicing results in multiple transcript variants, with one variant encoding multiple distinct proteins, ATXN1 and Alt-ATXN1, due to the use of overlapping alternate reading frames. [provided by RefSeq, Nov 2017] Transcript Variant: This variant (1) encodes multiple distinct proteins due to the use of alternative translation initiation codons. The longer 815 aa protein (ATXN1, PMID:23705062) represents the canonical protein, while a shorter protein (Alt-ATXN1, PMID:23705062) that uses a different reading frame has also been described. This RefSeq represents the longer protein, ATXN1. Variant 2 also encodes ATXN1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Loss of Function of ATXN1 Increases Amyloid ?-Protein Levels by Potentiating ?-Secretase Processing of ?-Amyloid Precursor Protein *
,null,
The Journal of Biological Chemistry
,PubMed ID 20097758
[ATXN1]
|
|
Loss of Function of ATXN1 Increases Amyloid ß-Protein Levels by Potentiating ß-Secretase Processing of ß-Amyloid Precursor Protein
,Can Zhang, Andrew Browne, Daniel Child, Jason R. DiVito, Jesse A. Stevenson, and Rudolph E. Tanzi,
J. Biol. Chem., Mar 2010; 285: 8515 - 8526
[ATXN1]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222862 | ATXN1 (Myc-DDK-tagged)-Human ataxin 1 (ATXN1), transcript variant 1 |
CNY 5,968.00 |
|
| RC222862L1 | Lenti ORF clone of Human ataxin 1 (ATXN1), transcript variant 1, Myc-DDK-tagged |
CNY 8,368.00 |
|
| RC222862L2 | Lenti ORF clone of Human ataxin 1 (ATXN1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC222862L3 | Lenti ORF clone of Human ataxin 1 (ATXN1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC222862L4 | Lenti ORF clone of Human ataxin 1 (ATXN1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG222862 | ATXN1 (tGFP-tagged) - Human ataxin 1 (ATXN1), transcript variant 1 |
CNY 4,370.00 |
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