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RASGRP1 (NM_005739) Human Untagged Clone
CAT＃: SC314725
RASGRP1 (untagged)-Human RAS guanyl releasing protein 1 (calcium and DAG-regulated) (RASGRP1), transcript variant 1
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CALDAG-GEFI; CALDAG-GEFII; IMD64; RASGRP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC314725 representing NM_005739.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGCACCCTGGGCAAGGCGAGAGAGGCTCCGCGGAAACCTTCCCATGGCTGCAGAGCTGCCTCTAAA GCAAGACTAGAGGCAAAGCCAGCCAACAGCCCCTTCCCCTCCCATCCCAGCTTGGCCCACATCACCCAG TTCCGAATGATGGTGTCTCTGGGACATTTAGCCAAAGGAGCCAGCCTGGACGATCTCATTGACAGCTGC ATTCAATCTTTTGATGCAGATGGAAACCTGTGTCGAAGTAACCAACTGTTGCAAGTCATGCTGACCATG CACCGAATTGTCATCTCCTCTGCAGAACTGCTCCAAAAAGTTATCACCCTCTATAAGGATGCTTTGGCA AAGAATTCACCAGGACTTTGCCTGAAGATCTGTTATTTTGTAAGGTATTGGATAACAGAATTCTGGGTC ATGTTTAAAATGGACGCCAGCTTGACAGACACTATGGAGGAGTTTCAGGAACTGGTGAAAGCTAAGGGT GAGGAGTTACATTGCCGCCTGATTGACACAACTCAAATCAATGCCCGTGACTGGTCCAGGAAACTTACT CAAAGGATAAAATCAAATACCAGCAAGAAACGGAAAGTCTCCCTGCTCTTTGACCATCTGGAACCAGAA GAGCTATCCGAGCACCTCACCTACCTTGAGTTCAAGTCTTTCCGGAGGATATCGTTCTCTGATTATCAG AATTACCTTGTAAATAGCTGTGTGAAGGAAAACCCCACCATGGAGCGATCTATTGCTCTGTGCAACGGC ATCTCCCAGTGGGTACAACTGATGGTTCTCAGCCGCCCCACGCCGCAGCTCCGAGCAGAAGTCTTCATC AAGTTCATCCAGGTGGCTCAGAAGCTCCACCAACTACAGAACTTCAATACACTGATGGCTGTGATAGGT GGGCTGTGTCACAGCTCAATCTCGAGGCTCAAGGAGACAAGTTCGCATGTCCCACATGAAATCAATAAG GTTCTCGGTGAGATGACTGAGCTGCTGTCCTCCTCCAGAAACTACGACAATTACCGGCGAGCCTATGGA GAGTGCACCGACTTCAAGATCCCCATTCTGGGTGTGCATCTCAAGGACCTCATCTCCCTGTATGAAGCC ATGCCTGACTATCTGGAGGACGGGAAAGTGAACGTCCATAAGCTACTGGCCCTATACAATCATATCAGT GAATTGGTCCAGCTGCAAGAGGTGGCCCCACCCTTGGAGGCTAACAAGGACTTGGTACACTTGCTGACG TTATCCCTGGATCTTTACTACACTGAGGATGAAATCTATGAGCTTTCCTATGCCCGGGAACCAAGGAAC CACAGAGCTCCACCACTAACACCTTCAAAGCCACCAGTAGTAGTGGACTGGGCTTCTGGAGTGTCTCCC AAACCTGATCCAAAAACCATTAGCAAACACGTCCAGAGGATGGTGGATTCTGTCTTCAAGAACTATGAT CACGACCAGGATGGATACATTTCTCAGGAAGAATTTGAAAAGATTGCTGCGAGTTTTCCATTTTCCTTC TGTGTGATGGACAAAGACAGGGAAGGCCTCATCAGCAGGGATGAGATCACAGCCTACTTCATGAGAGCC AGCTCAATCTATTCCAAGCTGGGCCTGGGCTTTCCTCACAACTTCCAAGAGACCACCTACCTGAAGCCC ACTTTTTGTGACAACTGTGCTGGATTTCTCTGGGGAGTGATCAAACAAGGATATCGATGTAAAGACTGC GGGATGAACTGTCACAAACAATGCAAAGATCTGGTTGTGTTTGAGTGTAAGAAGCGAGCCAAGAACCCA GTAGCTCCCACAGAGAACAACACTTCTGTGGGGCCAGTGTCCAACCTTTGCTCATTGGGAGCCAAAGAT CTGCTCCATGCACCTGAGGAAGGACCTTTTACATTCCCTAATGGGGAGGCTGTGGAACATGGTGAGGAG AGTAAGGATCGGACCATCATGCTGATGGGAGTGTCCTCACAGAAGATTTCTCTTCGGCTGAAGAGGGCT GTTGCCCACAAGGCCACCCAGACTGAATCACAGCCTTGGATTGGCAGTGAGGGCCCTTCAGGTCCCTTT GTGCTGTCTTCCCCAAGGAAGACAGCCCAGGATACTCTATATGTGCTTCCCAGTCCCACCTCTCCATGT CCTAGCCCAGTCTTGGTCAGAAAGCGGGCTTTTGTCAAGTGGGAGAATAAAGACTCCCTCATAAAATCA AAGGAGGAGCTCCGTCACCTCAGACTGCCTACCTACCAAGAACTGGAACAGGAAATAAATACTCTGAAA GCAGATAATGATGCCCTAAAGATCCAACTGAAATATGCACAGAAGAAAATAGAATCCCTCCAGCTTGAA AAAAGCAATCATGTCTTAGCTCAAATGGAGCAGGGTGACTGTTCTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_005739 |
| Insert Size | 2394 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005739.3 |
| RefSeq Size | 5048 bp |
| RefSeq ORF | 2394 bp |
| Locus ID | 10125 |
| UniProt ID | O95267 |
| Domains | RasGEFN, RasGEF, EFh, DAG_PE-bind |
| Protein Pathways | MAPK signaling pathway, T cell receptor signaling pathway |
| MW | 90.4 kDa |
| Gene Summary | This gene is a member of a family of genes characterized by the presence of a Ras superfamily guanine nucleotide exchange factor (GEF) domain. It functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. It activates the Erk/MAP kinase cascade and regulates T-cells and B-cells development, homeostasis and differentiation. Alternatively spliced transcript variants encoding different isoforms have been identified. Altered expression of the different isoforms of this protein may be a cause of susceptibility to systemic lupus erythematosus (SLE). [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) represents the longer transcript, and encodes the longer isoform (a). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212473 | RASGRP1 (Myc-DDK-tagged)-Human RAS guanyl releasing protein 1 (calcium and DAG-regulated) (RASGRP1), transcript variant 1 |
CNY 5,840.00 |
|
| RC212473L1 | Lenti ORF clone of Human RAS guanyl releasing protein 1 (calcium and DAG-regulated) (RASGRP1), transcript variant 1, Myc-DDK-tagged |
CNY 8,240.00 |
|
| RC212473L2 | Lenti ORF clone of Human RAS guanyl releasing protein 1 (calcium and DAG-regulated) (RASGRP1), transcript variant 1, mGFP tagged |
CNY 8,240.00 |
|
| RC212473L3 | Lenti ORF clone of Human RAS guanyl releasing protein 1 (calcium and DAG-regulated) (RASGRP1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC212473L4 | Lenti ORF clone of Human RAS guanyl releasing protein 1 (calcium and DAG-regulated) (RASGRP1), transcript variant 1, mGFP tagged |
CNY 8,240.00 |
|
| RG212473 | RASGRP1 (tGFP-tagged) - Human RAS guanyl releasing protein 1 (calcium and DAG-regulated) (RASGRP1), transcript variant 1 |
CNY 7,440.00 |
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