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DNA polymerase eta (POLH) (NM_006502) Human Untagged Clone
CAT＃: SC314503
POLH (untagged)-Human polymerase (DNA directed), eta (POLH)
CNY 13,780.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | RAD30; RAD30A; XP-V; XPV |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC314503 representing NM_006502.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCTACTGGACAGGATCGAGTGGTTGCTCTCGTGGACATGGACTGTTTTTTTGTTCAAGTGGAGCAG CGGCAAAATCCTCATTTGAGGAATAAACCTTGTGCAGTTGTACAGTACAAATCATGGAAGGGTGGTGGA ATAATTGCAGTGAGTTATGAAGCTCGTGCATTTGGAGTCACTAGAAGTATGTGGGCAGATGATGCTAAG AAGTTATGTCCAGATCTTCTACTGGCACAAGTTCGTGAGTCCCGTGGGAAAGCTAACCTCACCAAGTAC CGGGAAGCCAGTGTTGAAGTGATGGAGATAATGTCTCGTTTTGCTGTGATTGAACGTGCCAGCATTGAT GAGGCTTACGTAGATCTGACCAGTGCTGTACAAGAGAGACTACAAAAGCTACAAGGTCAGCCTATCTCG GCAGACTTGTTGCCAAGCACTTACATTGAAGGGTTGCCCCAAGGCCCTACAACGGCAGAAGAGACTGTT CAGAAAGAGGGGATGCGAAAACAAGGCTTATTTCAATGGCTCGATTCTCTTCAGATTGATAACCTCACC TCTCCAGACCTGCAGCTCACCGTGGGAGCAGTGATTGTGGAGGAAATGAGAGCAGCCATAGAGAGGGAG ACTGGTTTTCAGTGTTCAGCTGGAATTTCACACAATAAGGTCCTGGCAAAACTGGCCTGTGGACTAAAC AAGCCCAACCGCCAAACCCTGGTTTCACATGGGTCAGTCCCACAGCTCTTCAGCCAAATGCCCATTCGC AAAATCCGTAGTCTTGGAGGAAAGCTAGGGGCCTCTGTCATTGAGATCCTAGGGATAGAATACATGGGT GAACTGACCCAGTTCACTGAATCCCAGCTCCAGAGTCATTTTGGGGAGAAGAATGGGTCTTGGCTATAT GCCATGTGCCGAGGGATTGAACATGATCCAGTTAAACCCAGGCAACTACCCAAAACCATTGGCTGTAGT AAGAACTTCCCAGGAAAAACAGCTCTTGCTACTCGGGAACAGGTACAATGGTGGCTGTTGCAATTAGCC CAGGAACTAGAGGAGAGACTGACTAAAGACCGAAATGATAATGACAGGGTAGCCACCCAGCTGGTTGTG AGCATTCGTGTACAAGGAGACAAACGCCTCAGCAGCCTGCGCCGCTGCTGTGCCCTTACCCGCTATGAT GCTCACAAGATGAGCCATGATGCATTTACTGTCATCAAGAACTGTAATACTTCTGGAATCCAGACAGAA TGGTCTCCTCCTCTCACAATGCTTTTCCTCTGTGCTACAAAATTTTCTGCCTCTGCCCCTTCATCTTCT ACAGACATCACCAGCTTCTTGAGCAGTGACCCAAGTTCTCTGCCAAAGGTGCCAGTTACCAGCTCAGAA GCTAAGACCCAGGGAAGTGGCCCAGCGGTGACAGCCACTAAGAAAGCAACCACGTCTCTGGAATCATTC TTCCAAAAAGCTGCAGAAAGGCAGAAAGTTAAAGAAGCTTCGCTTTCATCTCTTACTGCTCCCACTCAG GCTCCCATGAGCAATTCACCATCCAAGCCCTCATTACCTTTTCAAACCAGTCAAAGTACAGGAACTGAG CCCTTCTTTAAGCAGAAAAGTCTGCTTCTAAAGCAGAAACAGCTTAATAATTCTTCAGTTTCTTCCCCC CAACAAAACCCATGGTCCAACTGTAAAGCATTACCAAACTCTTTACCAACAGAGTATCCAGGGTGTGTC CCTGTTTGTGAAGGGGTGTCGAAGCTAGAAGAATCCTCTAAAGCAACTCCTGCAGAGATGGATTTGGCC CACAACAGCCAAAGCATGCACGCCTCTTCAGCTTCCAAATCTGTGCTGGAGGTGACTCAGAAAGCAACC CCAAATCCAAGTCTTCTAGCTGCTGAGGACCAAGTGCCCTGTGAGAAGTGTGGCTCCCTGGTACCGGTA TGGGATATGCCAGAACACATGGACTATCATTTTGCATTGGAGTTGCAGAAATCCTTTTTGCAGCCCCAC TCTTCAAACCCCCAGGTTGTTTCTGCCGTATCTCATCAAGGCAAAAGAAATCCCAAGAGCCCTTTGGCC TGCACTAATAAACGCCCCAGGCCTGAGGGCATGCAAACATTGGAATCATTTTTTAAGCCATTAACACAT TAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_006502 |
| Insert Size | 2142 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006502.2 |
| RefSeq Size | 8412 bp |
| RefSeq ORF | 2142 bp |
| Locus ID | 5429 |
| UniProt ID | Q9Y253 |
| Domains | IMS |
| Protein Families | Druggable Genome |
| MW | 78.4 kDa |
| Gene Summary | This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217354 | POLH (Myc-DDK-tagged)-Human polymerase (DNA directed), eta (POLH) |
CNY 5,224.00 |
|
| RC217354L1 | Lenti ORF clone of Human polymerase (DNA directed), eta (POLH), Myc-DDK-tagged |
CNY 7,624.00 |
|
| RC217354L2 | Lenti ORF clone of Human polymerase (DNA directed), eta (POLH), mGFP tagged |
CNY 7,700.00 |
|
| RC217354L3 | Lenti ORF clone of Human polymerase (DNA directed), eta (POLH), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC217354L4 | Lenti ORF clone of Human polymerase (DNA directed), eta (POLH), mGFP tagged |
CNY 7,700.00 |
|
| RG217354 | POLH (tGFP-tagged) - Human polymerase (DNA directed), eta (POLH) |
CNY 6,824.00 |
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