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Calpastatin (CAST) (NM_001750) Human Untagged Clone
CAT＃: SC314485
CAST (untagged)-Human calpastatin (CAST), transcript variant 1
CNY 5,192.00
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CNY 1,999.00
CNY 2,700.00
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Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BS-17; PLACK |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_001750 edited
ATGTCCCAGCCCGGCCAGAAGCCCGCCGCCTCCCCGCGGCCCCGGCGAGCAGCCGCCGCC CGCCGCACCCATGAGCATGTCAGTGAAAAAACCAGTGAATCGCCTTCCAAACCAGGAGAA AAGAAAGGATCAGATGAGAAAAAAGCAGCAAGCCTCGGCAGCAGTCAATCCTCCAGAACC TATGCTGGTGGAACAGCCTCGGCCACCAAGGTGTCAGCTTCCTCTGGTGCAACCAGCAAG TCTTCCAGTATGAATCCCACAGAAACCAAGGCCATTCCAGTCAGCCAACAGATGGAAGGA CCACATCTTCCTAACAAGAAAAAACACAAAAAACAGGCTGTAAAAACAGAACCTGAGAAG AAGTCACAGTCAACCAAGCTGTCTGTGGTTCATGAGAAAAAATCCCAAGAAGGAAAGCCA AAAGAACACACAGAGCCAAAAAGCCTACCCAAGCAGGCATCAGATACAGGAAGTAACGAT GCTCACAATAAAAAAGCAGTTTCCAGATCAGCTGAACAGCAGCCATCAGAGAAATCAACA GAACCAAAGACTAAACCACAAGACATGATTTCTGCTGGTGGAGAGAGTGTTGCTGGTATC ACTGCAATATCTGGCAAGCCGGGTGACAAGAAAAAAGAAAAGAAATCATTAACCCCAGCT GTGCCAGTTGAATCTAAACCGGATAAACCATCGGGAAAGTCAGGCATGGATGCTGCTTTG GATGACTTAATAGATACTTTAGGAGGACCTGAAGAAACTGAAGAAGAAAATACAACGTAT ACTGGACCAGAAGTTTCAGATCCAATGAGTTCCACCTACATAGAGGAATTGGGTAAAAGA GAAGTCACAATTCCTCCAAAATATAGGGAACTATTGGCTAAAAAGGAAGGGATCACAGGG CCTCCTGCAGACTCTTCGAAACCCATAGGGCCAGATGATGCTATAGACGCCTTGTCATCT GACTTCACCTGTGGGTCGCCTACAGCTGCTGGAAAGAAAACTGAAAAAGAGGAATCTACA GAAGTTTTAAAAGCTCAGTCATCAGGGACAGTCAGAAGTGCTGCTCCACCCCAAGAGAAG AAAAGAAAGGTGGAGAAGGATACAATGAGTGATCAAGCACTCGAGGCTCTGTCGGCTTCA CTGGGCACCCGGCAAGCAGAACCTGAGCTCGACCTCCGCTCAATTAAGGAAGTCGATGAG GCAAAAGCTAAAGAAGAAAAACTAGAGAAGTGTGGTGAGGATGATGAAACAATCCCATCT GAGTACAGATTAAAACCAGCCACGGATAAAGATGGAAAACCACTATTGCCAGAGCCTGAA GAAAAACCCAAGCCTCGGAGTGAATCAGAACTCATTGATGAACTTTCAGAAGATTTTGAC CGGTCTGAATGTAAAGAGAAACCATCTAAGCCAACTGAAAAGACAGAAGAATCTAAGGCC GCTGCTCCAGCTCCTGTGTCGGAGGCTGTGTGTCGGACCTCCATGTGTAGTATACAGTCA GCACCCCCTGAGCCGGCTACCTTGAAGGGCACAGTGCCAGATGATGCTGTAGAAGCCTTG GCTGATAGCCTGGGGAAAAAGGAAGCAGATCCAGAAGATGGAAAACCTGTGATGGATAAA GTCAAGGAGAAGGCCAAAGAAGAAGACCGTGAAAAGCTTGGTGAAAAAGAAGAAACAATT CCTCCTGATTATAGATTAGAAGAGGTCAAGGATAAAGATGGAAAGCCACTCCTGCCAAAA GAGTCTAAGGAACAGCTTCCACCCATGAGTGAAGACTTCCTTCTGGATGCTTTGTCTGAG GACTTCTCTGGTCCACAAAATGCTTCATCTCTTAAATTTGAAGATGCTAAACTTGCTGCT GCCATCTCTGAAGTGGTTTCCCAAACCCCAGCTTCAACGACCCAAGCTGGAGCCCCACCC CGTGATACCTCGCAGAGTGACAAAGACCTCGATGATGCCTTGGATAAACTCTCTGACAGT CTAGGACAAAGGCAGCCTGACCCAGATGAGAACAAACCAATGGAAGATAAAGTAAAGGAA AAAGCTAAAGCTGAACATAGAGACAAGCTTGGAGAAAGAGATGACACTATCCCACCTGAA TACAGACATCTCCTGGATGATAATGGACAGGACAAACCAGTGAAGCCACCTACAAAGAAA TCAGAGGATTCAAAGAAACCTGCAGATGACCAAGACCCCATTGATGCTCTCTCAGGAGAT CTGGACAGCTGTCCCTCCACTACAGAAACCTCACAGAACACAGCAAAGGATAAGTGCAAG AAGGCTGCTTCCAGCTCCAAAGCACCTAAGAATGGAGGTAAAGCGAAGGATTCAGCAAAG ACAACAGAGGAAACTTCCAAGCCAAAAGATGACTAA >OriGene 5' read for NM_001750 unedited
GGCAACGGAGATTTTGTATACGACTCACTTAGGGCGGCCGCGAATTCGGCACGAGCCTCC CTCTCTCCCTGGCAGGACTCCCCGCCAGGCCTCCCCGCCACTCTCCGCGGCGCATTCCGG GAGGCAGCGGCCGCAGCGGCCTCGCCATGTCCCAGCCCGGCCAGAAGCCCGCCGCCTCCC CGCGGCCCCGGCGAGCAGCCGCCGCCCGCCGCACCCATGAGCATGTCAGTGAAAAAACCA GTGAATCGCCTTCCAAACCAGGAGAAAAGAAAGGATCAGATGAGAAAAAAGCAGCAAGCC TCGGCAGCAGTCAATCCTCCAGAACCTATGCTGGTGGAACAGCCTCGGCCACCAAGGTGT CAGCTTCCTCTGGTGCAACCAGCAAGTCTTCCAGTATGAATCCCACAGAAACCAAGGCCA TTCCAGTCAGCCAACAGATGGAAGGACCACATCTTCCTAACAAGAAAAAACACAAAAAAC AGGCTGTAAAAACAGAACCTGAGAAGAAGTCACAGTCAACCAAGCTGTCTGTGGTTCATG AGAAAAAATCCCAAGAAGGAAAGCCAAAAGAACACACAGAGCCAAAAAGCCTACCCAAGC AGGCATCAGATACAGGAAGTAACGATGCTCACAATAAAAAAGCAGTTTCCAGATCAGCTG AACAGCAGCCATCAGAGAAATCAACAGAACCAAAGACTAAACCCACAGACATGATNTCTG CTGGTGGAGAGAGTGTTGCTGGTATCACTGCAATATCTGGCAA >Forward primer walk for NM_001750 unedited
AACAACTCTGATGCCTTGGAAAACTCTCTGACAGTCTAGGACAAAGGCAGCCTGACCCAG ATGAGAACAAACCAATGGAAGATAAAGTAAAGGAAAAAGCTAAAGCTGAACATAGAGACA AGCTTGGAGAAAGAGATGACACTATCCCACCTGAATACAGACATCTCCTGGATGATAATG GACAGGACAAACCAGTGAAGCCACCTACAAAGAAATCAGAGGATTCAAAGAAACCTGCAG ATGACCAAGACCCCATTGATGCTCTCTCAGGAGATCTGGACAGCTGTCCCTCCACTACAG AAACCTCACAGAACACAGCAAAGGATAAGTGCAAGAAGGCTGCTTCCAGCTCCAAAGCAC CTAAGAATGGAGGTAAAGCGAAGGATTCAGCAAAGACAACAGAGGAAACTTCCAAGCCAA AAGATGACTAAAGAAATACAAGTTAAGGTATCTGGTATCTGCATGTAAAATCTTCAGCTG GTGGATGGTGACTTTTGAAGAACAAAAGGCTTTGGCAACAGAAAACAATTGTTCTGGGTG ATTTCTAGAATGGTTTTTGTTGAGTCTCTGAACATCCTAAAATATTGGTTTGTTATTCTT TTCCAGAAAGAAAATGAATTTGACTGGTTCACCTGTGTACTGAGTATTGATAAAACTTTG AATTTTTTTAATTGCCTTCAATTGGGAGAGAACAGCTTTATATTTGTAAAAAATATATTT TGATAAAGTTTCTTAAACGCACCACCAAAAAACAAAAAAAAAAGCCTAAGTGAATTTTTG CACATTCTACACACAGTGCCTGGAACACCCACTTGTATTTACGTCCGACCTTAATTTTTT TTGTAGCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001750 |
| Insert Size | 4500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001750.4, NP_001741.3 |
| RefSeq Size | 2438 bp |
| RefSeq ORF | 2127 bp |
| Locus ID | 831 |
| UniProt ID | P20810 |
| Domains | Calpain_inhib |
| Gene Summary | The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211776 | CAST (Myc-DDK-tagged)-Human calpastatin (CAST), transcript variant 1 |
CNY 5,792.00 |
|
| RC211776L1 | Lenti ORF clone of Human calpastatin (CAST), transcript variant 1, Myc-DDK-tagged |
CNY 8,192.00 |
|
| RC211776L2 | Lenti ORF clone of Human calpastatin (CAST), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| RC211776L3 | Lenti ORF clone of Human calpastatin (CAST), transcript variant 1, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC211776L4 | Lenti ORF clone of Human calpastatin (CAST), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| RG211776 | CAST (tGFP-tagged) - Human calpastatin (CAST), transcript variant 1 |
CNY 7,392.00 |
|
| SC318276 | CAST (untagged)-Human calpastatin (CAST), transcript variant 1 |
CNY 15,300.00 |
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