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VPS16 (NM_080413) Human Untagged Clone
CAT＃: SC314454
VPS16 (untagged)-Human vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16), transcript variant 3
CNY 13,400.00
Product images
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DYT30; hVPS16 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC314454 representing NM_080413.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGACTGCTACACGGCGAACTGGAACCCACTCGGGGACTCTGCCTTTTACCGGAAATATGAGCTGTAC AGCATGGACTGGGACCTGAAGGAGGAACTCAGGGATTGCCTGGTGGCTGCTGCACCCTATGGGGGCCCC ATTGCACTGCTGAGGAACCCCTGGCGGAAGGAGAAAGCTGCTAGTGTGAGGCCAGTGCTCGATATATAC TCTGCTTCCGGCATGCCTCTGGCCAGCCTGCTGTGGAAGAGTGGACCCGTGGTGTCCCTGGGCTGGTCA GCTGAGGAGGAGCTGCTCTGTGTGCAGGAAGATGGTGCTGTACTGGTTTATGGGCTTCATGGTGACTTC CGGAGACACTTCAGCATGGGCAATGAAGTGCTCCAGAACCGGGTTCTGGATGCCCGGATCTTTCACACT GAGTTTGGTTCCGGAGTGGCCATCCTCACAGGGGCCCACCGCTTCACCCTCAGTGCCAATGTGGGTGAC CTCAAACTCCGCCGGATGCCAGAGGTGCCAGGTCTGCAAAGTGCACCCTCCTGCTGGACTGTGCTGTGC CAGGACCGAGTGGCACACATTCTTCTGGCTGTGGGGCCTGACCTTTACCTCTTGGACCATGCAGCCTGC TCCGCAGTGACGCCCCCTGGCCTGGCCCCAGGAGTAAGCAGCTTCCTACAGATGGCTGTCTCCTTCACC TACCGACACCTGGCACTCTTCACAGACACAGGCTACATCTGGATGGGGACAGCATCACTCAAGGAGAAG CTATGTGAGTTCAACTGCAACATCCGGGCACCTCCAAAGCAGATGGTCTGGTGCAGCCGTCCTCGTAGC AAGGAGAGGGCCGTGGTGGTGGCCTGGGAAAGGCGGCTGATGGTGGTGGGCGATGCACCCGAGAGCATC CAATATAAGCAGCTCACCATCCAGGTGCTGCTGGACAGGCTCGTGTTGCGGAGACTTTACCCCCTGGCC ATCCAGATATGCGAGTACTTGCGCCTTCCTGAAGTACAGGGCGTCAGCAGGATCCTGGCCCACTGGGCC TGCTACAAGGTGCAACAGAAGGATGTCTCAGATGAGGATGTGGCTCGAGCCATTAACCAGAAGCTGGGG GACACGCCTGGTGTCTCTTACTCCGACATTGCTGCACGAGCCTATGGTTGTGGCCGCACGGAGCTGGCC ATCAAGCTGCTGGAGTATGAGCCACGCTCAGGGGAGCAGGTACCCCTTCTCCTAAAGATGAAGAGGAGC AAACTGGCACTAAGCAAGGCCATCGAGAGCGGGGACACTGACCTGGTGTTCACGGTGTTGCTGCACCTG AAGAACGAGCTGAACCGAGGAGATTTTTTCATGACCCTTCGGAATCAGCCCATGGCCCTCAGTTTGTAC CGACAGTTCTGTAAGCATCAGGAGCTAGAGACGCTGAAGGACCTTTACAATCAGGATGACAATCACCAG GAATTGGGCAGCTTCCACATCCGAGCCAGCTATGCTGCAGAAGAGCGTATTGAGGGGCGAGTAGCAGCT CTGCAGACAGCCGCCGATGCCTTCTACAAGGCCAAGAATGAGTTTGCAGCCAAGGCTACAGAGGATCAA ATGCGGCTCCTACGGCTGCAGCGGCGCCTAGAAGACGAGCTGGGGGGCCAGTTCCTAGACCTGTCTCTA CATGACACAGTTACCACCCTCATTCTTGGCGGTCACAACAAGCGTGCAGAGCAGCTGGCACGTGACTTC CGCATCCCTGACAAGAGGCTCTGGTGGCTGAAGCTGACTGCCCTGGCAGATTTGGAAGATTGGGAAGAG CTAGAGAAGTTTTCCAAGAGCAAGAAATCACCCATTGGCTACCTGCCTTTTGTGGAGATCTGCATGAAA CAACATAACAAATACGAAGCCAAGAAGTATGCTTCCCGCGTGGGTCCCGAGCAGAAGGTCAAGGCTTTG CTTCTTGTTGGCGATGTGGCTCAGGCTGCAGATGTGGCCATCGAACACCGGAATGAGGCTGAGCTGAGC CTCGTATTGTCCCACTGCACGGGAGCCACAGATGGGGCCACAGCTGACAAGATTCAACGGGCCAGGGCA CAAGCCCAGAAGAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_080413 |
| Insert Size | 2088 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_080413.2 |
| RefSeq Size | 2341 bp |
| RefSeq ORF | 2088 bp |
| Locus ID | 64601 |
| UniProt ID | Q9H269 |
| Domains | Vps16_C, Vps16_N |
| Protein Families | Druggable Genome |
| MW | 78.3 kDa |
| Gene Summary | Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps16 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009] Transcript Variant: This variant (3) lacks an internal sequence segment compared to transcript variant 1. It thus encodes a shorter isoform (3) which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217614 | VPS16 (Myc-DDK-tagged)-Human vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16), transcript variant 3 |
CNY 5,088.00 |
|
| RC217614L3 | Lenti-ORF clone of VPS16 (Myc-DDK-tagged)-Human vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16), transcript variant 3 |
CNY 9,220.00 |
|
| RC217614L4 | Lenti-ORF clone of VPS16 (mGFP-tagged)-Human vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16), transcript variant 3 |
CNY 9,220.00 |
|
| RG217614 | VPS16 (tGFP-tagged) - Human vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16), transcript variant 3 |
CNY 8,080.00 |
