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Adducin 2 (ADD2) (NM_017488) Human Untagged Clone
CAT＃: SC314313
ADD2 (untagged)-Human adducin 2 (beta) (ADD2), transcript variant 4
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ADDB |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC314313 representing NM_017488.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGCGAAGAGACGGTCCCCGAGGCTGCCTCGCCGCCGCCCCCGCAGGGGCAGCCTTACTTTGACCGC TTCTCAGAGGACGACCCCGAGTACATGCGCCTTCGCAACCGGGCGGCGGACCTGCGGCAGGACTTCAAC CTGATGGAGCAGAAGAAGCGCGTCACCATGATCCTGCAGAGTCCCTCTTTCAGGGAGGAGCTGGAAGGC CTCATCCAGGAGCAGATGAAGAAGGGGAACAACTCCTCCAACATCTGGGCCCTGCGACAGATCGCGGAC TTCATGGCCAGCACCTCCCACGCAGTCTTCCCGACATCTTCCATGAATGTCTCCATGATGACGCCTATC AATGACCTCCACACAGCTGACTCCCTGAACCTGGCCAAAGGGGAGCGGCTCATGCGGTGCAAGATCAGC AGTGTCTACCGACTCCTGGACCTCTATGGCTGGGCCCAGCTGAGTGACACCTATGTCACGTTGAGAGTC AGCAAGGAGCAGGACCACTTCCTGATCAGCCCTAAGGGAGTTTCTTGCAGTGAAGTCACAGCGTCCAGC CTGATCAAGGTGAACATTCTGGGAGAGGTGGTGGAGAAGGGCAGCAGCTGCTTCCCAGTGGACACCACA GGCTTCTGTCTGCACTCGGCCATCTATGCAGCGAGGCCCGACGTGCGCTGCATCATCCACCTGCACACA CCGGCCACAGCAGCGGTGTCGGCCATGAAGTGGGGCCTCCTGCCTGTCTCCCACAATGCCCTGCTGGTG GGGGACATGGCCTATTATGACTTCAATGGGGAAATGGAGCAGGAAGCCGATCGGATCAACCTGCAGAAG TGCCTTGGACCCACCTGCAAGATCCTGGTGCTAAGAAACCATGGAGTGGTTGCTCTGGGTGACACGGTA GAGGAGGCATTTTACAAGATCTTCCACCTGCAGGCTGCATGTGAGATACAGGTGTCGGCTCTGTCCAGT GCCGGGGGAGTGGAGAACCTCATCCTCCTGGAGCAGGAGAAGCACCGGCCCCATGAGGTGGGCTCCGTG CAGTGGGCCGGGAGCACCTTTGGGCCTATGCAGAAGAGTCGGCTGGGGGAGCATGAGTTTGAGGCCCTC ATGAGGATGCTGGACAACCTGGGCTACAGAACAGGTTACACGTATCGCCACCCCTTTGTTCAAGAGAAA ACCAAACACAAAAGTGAGGTGGAGATTCCAGCCACGGTCACAGCCTTCGTGTTTGAGGAGGACGGTGCC CCGGTGCCCGCCCTGCGACAGCATGCCCAGAAGCAGCAGAAGGAGAAGACCCGCTGGCTCAATACGCCC AACACCTACCTGCGGGTCAATGTGGCCGATGAGGTCCAGAGGAGCATGGGCAGCCCCCGACCCAAGACC ACGTGGATGAAGGCTGACGAGGTGGAGAAATCCAGCAGTGGCATGCCGATTCGCATCGAAAACCCAAAC CAATTTGTGCCTCTCTATACTGACCCCCAGGAAGTACTGGAGATGAGGAACAAGATTCGAGAACAAAAC CGACAAGATGTGAAGTCAGCGGGGCCTCAGTCCCAGCTCCTGGCGAGCGTCATTGCCGAGAAGAGCCGA AGCCCGTCTACAGAGAGCCAGCTGATGTCCAAGGGAGACGAGGATACCAAAGACGATTCAGAGGAGACG GTGCCCAACCCCTTCAGCCAACTCACTGACCAGGAGTTGGAGGAGTACAAGAAAGAGGTGGAGAGGAAG AAACTAGAACTTGATGAGACAGGACAGGAACGAGAGCCAGGCTCTGGTCCGGCCGTGTGCGAGTTCTTC AGCGTTGCCCTCCACATCTGGAGTAACATATTGGAGAGAAAGAAACTGCCCCAGAAGAGCCTGGCTCAC CTGCAAAGTCTGCACCTGCTTCTCCAGTGCAGAGCCCAGCGAAGGAGGCAGAGACAAAGAGCCCTTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_017488 |
| Insert Size | 1932 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_017488.3 |
| RefSeq Size | 4119 bp |
| RefSeq ORF | 1932 bp |
| Locus ID | 119 |
| UniProt ID | P35612 |
| MW | 72.7 kDa |
| Gene Summary | Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jun 2010] Transcript Variant: This variant (4) includes an alternate exon in the 3' coding region, which results in a frameshift, compared to variant 1. The resulting protein (isoform e) has a distinct C-terminus and is shorter than isoform a. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207895 | ADD2 (Myc-DDK-tagged)-Human adducin 2 (beta) (ADD2), transcript variant 4 |
CNY 4,784.00 |
|
| RC207895L3 | Lenti ORF clone of Human adducin 2 (beta) (ADD2), transcript variant 4, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC207895L4 | Lenti ORF clone of Human adducin 2 (beta) (ADD2), transcript variant 4, mGFP tagged |
CNY 6,460.00 |
|
| RG207895 | ADD2 (tGFP-tagged) - Human adducin 2 (beta) (ADD2), transcript variant 4 |
CNY 6,384.00 |
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