United States
Germany
Japan
United Kingdom
China
MBD1 (NM_015846) Human Untagged Clone
CAT＃: SC314167
MBD1 (untagged)-Human methyl-CpG binding domain protein 1 (MBD1), transcript variant 1
CNY 9,790.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CXXC3; PCM1; RFT |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_015846 edited
ATGGCTGAGGACTGGCTGGACTGCCCGGCCCTGGGCCCTGGCTGGAAGCGCCGCGAAGTC TTTCGCAAGTCAGGGGCCACCTGTGGACGCTCAGACACCTATTACCAGAGCCCCACAGGA GACAGGATCCGAAGCAAAGTTGAGCTGACTCGATACCTGGGCCCTGCGTGTGATCTCACC CTCTTCGACTTCAAACAAGGCATCTTGTGCTATCCAGCCCCCAAGGCCCATCCCGTGGCA GTTGCCAGCAAGAAGCGAAAGAAGCCTTCAAGGCCAGCCAAGACTCGGAAACGTCAGGTT GGACCCCAGAGTGGTGAGGTCAGGAAGGAGGCCCCGAGGGATGAGACCAAGGCTGACACT GACACAGCCCCAGCTTCATTCCCTGCTCCTGGGTGCTGTGAGAACTGTGGAATCAGCTTC TCAGGGGATGGCACCCAAAGGCAGCGGCTCAAAACGTTGTGCAAAGACTGTCGAGCACAG AGAATTGCCTTCAACCGGGAACAGAGAATGTTTAAGCGTGTGGGCTGTGGGGAGTGTGCA GCCTGCCAGGTAACAGAAGACTGTGGGGCCTGCTCCACCTGCCTCCTGCAGCTGCCCCAT GATGTGGCATCGGGGCTGTTCTGCAAGTGTGAACGGAGACGCTGCCTCCGGATTGTGGAA AGGAGCCGAGGGTGTGGAGTATGCCGGGGCTGTCAGACCCAAGAGGATTGTGGCCATTGC CCCATCTGCCTTCGCCCTCCCCGCCCTGGTCTCAGGCGCCAGTGGAAATGTGTCCAGCGA CGTTGCCTACGGGGTAAACATGCCCGCCGCAAGGGAGGCTGTGACTCCAAGATGGCTGCC AGGCGGCGCCCCGGAGCCCAGCCACTGCCTCCACCACCCCCATCACAGTCCCCAGAGCCC ACAGAGCCGCACCCCAGAGCCCTGGCCCCCTCGCCACCTGCCGAGTTCATCTATTACTGT GTAGACGAGGACGAGCTACAGCCCTACACGAACCGCCGGCAGAACCGCAAGTGCGGGGCC TGTGCAGCCTGCCTACGGCGGATGGACTGTGGCCGCTGCGACTTCTGCTGCGACAAGCCC AAATTCGGGGGCAGCAACCAGAAGCGCCAGAAGTGTCGTTGGCGCCAATGCCTGCAGTTT GCCATGAAGCGGCTGCTGCCCAGTGTCTGGTCAGAGTCTGAGGATGGGGCAGGATCGCCC CCACCTTACCGTCGTCGAAAGAGGCCCAGCTCTGCCCGACGGCACCATCTTGGCCCTACC TTGAAGCCCACCTTGGCTACACGCACAGCCCAACCAGACCATACCCAGGCTCCAACGAAG CAGGAAGCAGGTGGTGGCTTTGTGCTGCCCCCGCCTGGCACTGACCTTGTGTTTTTACGG GAAGGCGCAAGCAGTCCTGTGCAGGTGCCGGGCCCTGTTGCAGCTTCCACAGAAGCCCTG TTGCAGGAGGCCCAGTGCTCTGGCCTGAGTTGGGTTGTGGCCTTACCCCAGGTGAAGCAA GAGAAGGCGGATACCCAGGACGAGTGGACACCAGGCACAGCTGTCCTGACTTCTCCCGTA TTGGTGCCTGGCTGCCCTAGCAAGGCAGTAGACCCAGGCCTGCCTTCTGTGAAGCAAGAG CCACCTGACCCAGAGGAGGACAAGGAGGAGAACAAGGATGATTCTGCCTCCAAATTGGCC CCAGAGGAAGAGGCAGGAGGGGCTGGCACACCCGTGATCACGGAGATTTTCAGCCTGGGT GGAACCCGCTTCCGAGATACAGCAGTCTGGTTGCCAAGGTCCAAAGACCTTAAAAAACCT GGAGCTAGAAAGCAGTAG |
| Restriction Sites | Please inquire |
| ACCN | NM_015846 |
| Insert Size | 3200 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015846.2, NP_056671.2 |
| RefSeq Size | 2961 bp |
| RefSeq ORF | 1818 bp |
| Locus ID | 4152 |
| UniProt ID | Q9UIS9 |
| Protein Families | Druggable Genome, Transcription Factors |
| Gene Summary | The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains multiple domains: MBD at the N-terminus that functions both in binding to methylated DNA and in protein interactions; several CXXC-type zinc finger domains that mediate binding to non-methylated CpG dinucleotides; transcriptional repression domain (TRD) at the C-terminus that is involved in transcription repression and in protein interactions. Numerous alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Feb 2011] Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform 1. Variants 1 and 14 encode the same isoform. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220914 | MBD1 (Myc-DDK-tagged)-Human methyl-CpG binding domain protein 1 (MBD1), transcript variant 1 |
CNY 4,504.00 |
|
| RC220914L3 | Lenti ORF clone of Human methyl-CpG binding domain protein 1 (MBD1), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC220914L4 | Lenti ORF clone of Human methyl-CpG binding domain protein 1 (MBD1), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RG220914 | MBD1 (tGFP-tagged) - Human methyl-CpG binding domain protein 1 (MBD1), transcript variant 1 |
CNY 5,040.00 |
