PRKAG2 (AJ249976) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AAKG; AAKG2; CMH6; H91620p; WPWS
Vector	pCMV6 series
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AJ249976, the custom clone sequence may differ by one or more nucleotides ATGGGAAGCGCGGTTATGGACACCAAGAAGAAAAAAGATGTTTCCAGCCCCGGCGGGAGC GGCGGCAAGAAAAATGCCAGCCAGAAGAGGCGTTCGCTGCGCGTGCACATTCCGGACCTG AGCTCCTTCGCCATGCCGCTCCTGGACGGAGACCTGGAGGGTTCCGGAAAGCATTCCTCT CGAAAGGTGGACAGCCCCTTCGGCCCGGGCAGCCCCTCCAAAGGGTTCTTCTCCAGAGGC CCCCAGCCCCGGCCCTCCAGCCCCATGTCTGCACCTGTGAGGCCCAAGACCAGCCCCGGC TCTCCCAAAACCGTGTTCCCGTTCTCCTACCAGGAGTCCCCGCCACGCTCCCCTCGACGC ATGAGCTTCAGTGGGATCTTCCGCTCCTCCTCCAAAGAGTCTTCCCCCAACTCCAACCCT GCTACCTCGCCCGGGGGCATCAGGTTTTTCTCCCGCTCCAGAAAAACCTCCGGCCTCTCC TCCTCTCCGTCAACACCCACCCAAGTGACCAAGCAGCACACGTTTCCCCTGGAATCCTAT AAGCACGAGCCTGAACGGTTAGAGAATCGCATCTATGCCTCGTCTTCCCCCCCGGACACA GGGCAGAGGTTCTGCCCGTCTTCCTTCCAGAGCCCGACCAGGCCTCCACTGGCATCACCG ACACACTATGCTCCCTCCAAAGCCGCGGCGCTGGCGGCGGCCCTGGGACCCGCGGAAGCC GGCATGCTGGAGAAGCTGGAGTTCGAGGACGAAGCAGTAGAAGACTCAGAAAGTGGTGTT TACATGCGATTCATGAGGTCACACAAGTGTTATGACATCGTTCCAACCAGTTCAAAGCTT GTTGTCTTTGATACTACATTACAAGTTAAAAAGGCCTTCTTTGCTTTGGTAGCCAACGGT GTCCGAGCAGCGCCACTGTGGGAGAGTAAAAAACAAAGTTTTGTAGGAATGCTAACAATT ACAGATTTCATAAATATACTACATAGATACTATAAATCACCTATGGTACAGATTTATGAA TTAGAGGAACATAAAATTGAAACATGGAGGGAGCTTTATTTACAAGAAACATTTAAGCCT TTAGTGAATATATCTCCAGATGCAAGCCTCTTCGATGCTGTATACTCCTTGATCAAAAAT AAAATCCACAGATTGCCCGTTATTGACCCTATCAGTGGGAATGCACTTTATATACTTACC CACAAAAGAATCCTCAAGTTCCTCCAGCTTTTTATGTCTGATATGCCAAAGCCTGCTTTC ATGAAGCAGAACCTGGATGAGCTTGGAATAGGAACGTACCACAACATTGCCTTCATACAT CCAGACACTCCCATCATCAAAGCCTTGAACATATTTGTGGAAAGACGAATATCAGCTCTG CCTGTTGTGGATGAGTCAGGAAAAGTTGTAGATATTTATTCCAAATTTGATGTAATTAAT CTTGCTGCTGAGAAAACATACAATAACCTAGATATCACGGTGACCCAGGCCCTTCAGCAC CGTTCACAGTATTTTGAAGGTGTTGTGAAGTGCAATAAGCTGGAAATACTGGAGACCATC GTGGACAGAATAGTAAGAGCTGAGGTCCATCGGTTGGTGGTGGTAAATGAAGCAGATAGT ATTGTGGGTATTATTTCCCTGTCGGACATTCTGCAAGCCCTGATCCTCACACCAGCAGGT GCCAAACAAAAGGAGACAGAAACGGAG
Restriction Sites	Please inquire
ACCN	AJ249976
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AJ249976.1, CAB65116.1
RefSeq Size	2062 bp
RefSeq ORF	1710 bp
Locus ID	51422
Domains	CBS
Protein Families	Druggable Genome
Protein Pathways	Adipocytokine signaling pathway, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway
Gene Summary	AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This gene is a member of the AMPK gamma subunit family. Mutations in this gene have been associated with Wolff-Parkinson-White syndrome, familial hypertrophic cardiomyopathy, and glycogen storage disease of the heart. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jan 2015]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.