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POLE2 (NM_002692) Human Untagged Clone
CAT＃: SC313869
POLE2 (untagged)-Human polymerase (DNA directed), epsilon 2 (p59 subunit) (POLE2), transcript variant 1
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DPE2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC313869 representing NM_002692.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGCCGGAGCGGCTGCGGAGCCGGGCGCTCTCCGCCTTCAAGTTGCGGGGCTTGCTGCTCCGTGGT GAAGCTATTAAGTACCTCACAGAAGCTCTTCAGTCTATCAGTGAATTAGAGCTTGAAGATAAACTGGAA AAGATAATTAATGCAGTTGAGAAGCAACCCTTGTCATCAAACATGATTGAACGATCTGTGGTGGAAGCA GCAGTCCAGGAATGCAGTCAGTCTGTTGATGAAACTATAGAGCACGTTTTCAATATCATAGGAGCATTT GATATTCCACGCTTTGTGTACAATTCAGAAAGAAAAAAATTTCTTCCTCTGTTAATGACCAACCACCCT GCACCAAATTTATTTGGAACACCAAGAGATAAAGCAGAGATGTTTCGTGAGCGATATACCATTTTGCAC CAGAGGACCCACAGGCATGAATTATTTACTCCTCCGGTGATAGGTTCTCACCCTGATGAAAGCGGAAGC AAATTCCAGCTTAAAACAATAGAAACCTTATTGGGTAGTACAACCAAAATCGGAGATGCGATTGTTCTT GGAATGATAACGCAGTTAAAAGAGGGAAAATTTTTTCTGGAAGATCCTACTGGAACAGTCCAACTAGAC CTTAGTAAAGCTCAGTTCCATAGTGGTTTATACACAGAGGCATGCTTTGTCTTAGCAGAAGGTTGGTTT GAAGATCAAGTGTTTCATGTCAATGCCTTTGGATTTCCACCCACTGAGCCCTCTAGTACTACTAGGGCA TACTATGGAAATATTAATTTTTTTGGAGGTCCTTCTAATACATCTGTGAAGACTTCTGCAAAACTAAAA CAGCTAGAAGAGGAGAATAAAGATGCTATGTTTGTGTTTTTATCTGATGTTTGGTTGGACCAGGTGGAA GTATTGGAAAAACTTCGCATAATGTTTGCTGGTTATTCACCAGCACCTCCAACCTGCTTTATTCTGTGT GGTAATTTTTCATCTGCACCATATGGAAAAAATCAAGTTCAAGCTTTGAAAGATTCCCTAAAAACTTTG GCAGATATAATATGTGAATACCCAGATATTCACCAAAGTAGTCGTTTTGTGTTTGTACCTGGTCCAGAG GATCCTGGATTTGGTTCCATCTTACCAAGGCCACCACTTGCTGAAAGCATCACTAATGAATTCAGACAA AGGGTACCATTTTCAGTTTTTACTACTAATCCTTGCAGAATTCAGTACTGTACACAGGAAATTACTGTC TTCCGTGAAGACTTAGTAAATAAAATGTGCAGAAACTGCGTCCGTTTTCCTAGCAGCAATTTGGCTATT CCTAATCACTTTGTAAAGACTATCTTATCCCAAGGACATCTGACTCCCCTACCTCTTTATGTCTGCCCA GTGTATTGGGCATATGACTATGCTTTGAGAGTGTATCCTGTGCCCGATCTACTTGTCATTGCAGACAAA TATGATCCTTTCACTACGACAAATACCGAATGCCTCTGCATAAACCCTGGCTCTTTTCCAAGAAGTGGA TTTTCATTCAAAGTTTTTTATCCTTCTAATAAGACAGTAGAAGATAGCAAACTTCAAGGCTTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_002692 |
| Insert Size | 1584 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002692.3 |
| RefSeq Size | 1861 bp |
| RefSeq ORF | 1584 bp |
| Locus ID | 5427 |
| UniProt ID | P56282 |
| Domains | DNA_pol_E_B |
| Protein Pathways | Base excision repair, DNA replication, Metabolic pathways, Nucleotide excision repair, Purine metabolism, Pyrimidine metabolism |
| MW | 59.5 kDa |
| Gene Summary | DNA polymerase epsilon, which is involved in DNA repair and replication, is composed of a large catalytic subunit and a small accessory subunit. The protein encoded by this gene represents the small subunit (B). Defects in this gene have been linked to colorectal cancer and to combined immunodeficiency. [provided by RefSeq, Jan 2017] Transcript Variant: This variant (1) encodes the longest isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222113 | POLE2 (Myc-DDK-tagged)-Human polymerase (DNA directed), epsilon 2 (p59 subunit) (POLE2), transcript variant 1 |
CNY 3,928.00 |
|
| RC222113L3 | Lenti ORF clone of Human polymerase (DNA directed), epsilon 2 (p59 subunit) (POLE2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC222113L4 | Lenti ORF clone of Human polymerase (DNA directed), epsilon 2 (p59 subunit) (POLE2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG222113 | POLE2 (tGFP-tagged) - Human polymerase (DNA directed), epsilon 2 (p59 subunit) (POLE2), transcript variant 1 |
CNY 5,528.00 |
