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SESN3 (NM_144665) Human Untagged Clone
CAT＃: SC313706
SESN3 (untagged)-Human sestrin 3 (SESN3)
CNY 7,890.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | SEST3 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_144665 edited
CCTAGCCTGGCGGGGGCGGGGGTCTGGAAGAGGGGGCGGGTGGAACAACGGGCCGGTGAG GAGCAGCCGCCGCCGTCTCCGCCGTCGCTGGGGCAGCTGCCGCGGTGGTCGCCTCTGGCA GTGCCGCTAGCTTTCAGGCAGTCGCCTCTCCTCCCGACATCCCGCCTTGAGGAGGCTGAG GCGGAATCGCGGCTGGCGGCGGGGGGACGGACCGGCCCTGCAGTGGCCGCAGTGACAGCC CGGACCCGGCCCTCCGCCCGCTCTCGCCTCGGCGCCCGCAGCCACGATGAACCGGGGCGG CGGCAGCCCGTCGGCCGCCGCCAACTACCTGCTCTGTACCAACTGCCGGAAAGTGCTGCG GAAGGATAAAAGAATCAGAGTGTCTCAACCCTTGACAAGAGGACCAAGTGCCTTTATTCC AGAGAAGGAAGTTGTCCAAGCAAACACAGTGGATGAACGTACTAACTTTCTTGTGGAAGA ATACTCTACATCCGGTCGTCTGGACAACATCACACAGGTCATGAGTTTACACACTCAGTA CCTGGAGTCTTTCTTGCGGAGCCAGTTTTACATGTTGCGCATGGATGGTCCCCTTCCTCT ACCATACAGGCACTATATTGCAATAATGGCTGCAGCTAGACATCAGTGTTCTTACTTAAT AAACATGCATGTGGATGAATTTTTAAAGACTGGAGGTATTGCTGAGTGGTTGAATGGTTT GGAATATGTGCCACAAAGACTGAAAAATCTTAATGAAATTAATAAGCTGCTAGCACATCG ACCTTGGCTGATCACAAAAGAGCACATTCAGAAACTTGTCAAAACTGGAGAAAATAATTG GTCTCTGCCTGAACTGGTACATGCTGTGGTCCTCCTGGCACATTATCATGCTTTGGCAAG CTTTGTTTTTGGTAGTGGTATCAATCCAGAGAGAGATCCAGAAATCTCCAATGGATTCAG GCTAATATCAGTCAACAATTTCTGCGTTTGTGATCTTGCTAATGACAACAACATAGAGAA TGCATCTCTTTCAGGCAGCAACTTTGGGATTGTGGATTCTCTAAGTGAGCTAGAGGCCTT AATGGAAAGGATGAAAAGACTTCAAGAAGAAAGGGAAGATGAAGAGGCGTCTCAAGAAGA AATGAGCACTCGTTTTGAAAAGGAGAAGAAAGAAAGTCTTTTTGTGGTCTCTGGAGATAC TTTTCATTCATTTCCTCATTCAGATTTTGAGGATGACATGATTATAACATCTGATGTCTC TCGATATATTGAAGACCCTGGTTTTGGGTATGAAGACTTTGCCAGACGAGGAGAAGAGCA TTTGCCAACATTCCGAGCTCAGGACTATACCTGGGAAAATCATGGGTTCTCCCTGGTGAA CAGACTTTATTCTGACATTGGACATCTTCTTGATGAAAAGTTTCGGATGGTCTACAATCT CACATATAACACTATGGCCACCCATGAGGATGTTGACACAACCATGCTGCGCAGAGCTTT ATTTAACTATGTTCACTGTATGTTTGGAATCAGGTATGATGACTATGATTATGGAGAAGT TAATCAATTACTTGAAAGAAGCCTGAAGGTTTACATTAAGACAGTGACCTGCTATCCTGA GAGAACTACAAAACGCATGTATGATAGTTACTGGCGGCAGTTCAAACACTCAGAAAAAGT TCATGTCAATCTACTTTTAATGGAAGCACGAATGCAAGCTGAACTTCTTTATGCTCTTCG TGCCATAACTCGGCATTTGACCTGAAGTATCACCCAAGGAAAATGTCATACATGTGTAAA GACCTTTCACATAAGATAAACATTGAAGCTATTTGTGATATAGCATCAAAAATTATTCAG TTCTCTAGTGTCAAAGTTTAGCCGTTTGTTTTTTTTTKTTTTTTGTTTTTTGCGGCTGTA ATGTGCAATGATGTGTTTTATTTTCCTTGATGCTTAACATTACTAACAATTGCAAAAATA ATACTGAGGAGCACTACTTTGCATTGTTTGTAGTTGGAGTTTTGGATACTGATCATAAAT CATGAATCTGGCGTATTAATGCTTAAAAAAAAAAAAAAAAAAAAAAAAAA >OriGene 5' read for NM_144665 unedited
CCGCGGGACCNCCNCCCCTTTCNCCCCCCGGTTCAGATTTGTATACGACTCACTTATAGG CGGCCGCGNATTCGGCACGAGCCTAGCCTGGCGGGGGCGGGGGTCTGGAAGAGGGGGCGG GTGGAACAACGGGCCGGTGAGGAGCAGCCGCCGCCGTCTCCGCCGTCGCTGGGGCAGCTG CCGCGGTGGTCGCCTCTGGCAGTGCCGCTAGCTTTCAGGCAGTCGCCTCTCCTCCCGACA TCCCGCCTTGAGGAGGCTGAGGCGGAATCGCGGCTGGCGGCGGGGGGACGGACCGGCCCT GCAGTGGCCGCAGTGACAGCCCGGACCCGGCCCTCCGCCCGCTCTCGCCTCGGCGCCCGC AGCCACGATGAACCGGGGCGGCGGCAGCCCGTCGGCCGCCGCCAACTACCTGCTCTGTAC CAACTGCCGGAAAGTGCTGCGGAAGGATAAAAGAATCAGAGTGTCTCAACCCTTGACAAG AGGACCAAGTGCCTTTATTCCAGAGAAGGAAGTTGTCCAAGCAAACACAGTGGATGAACG TACTAACTTTCTTGTGGAAGAATACTCTACATCCGGTCGTCTGGGACACATCACACAGGT CATGANGTTACACACTCAGTACCTGGAGTCTTTCTTGCGGAGCCAGTTTTACATGTTGCG CATGGATGGTCCCCTTCCTCTACCATACAGGCACTATATTGCAATAATGGCTGCAGCTAG ACATCAGTGTTCTTTACCTTATAACATGCATGTGGATGAATTTTTAAAGACTGGAGGTAT TGCTGAATGGTGAATGGTTTGGAATTTTGCCCCAAGACTGAAAATCTTATGAATTAATAG CTGTTACCCTTAACCTTGCTGATCCAAAGAACCTTCCTGACTTGTAAAACTGGAAAAAAA TTGGCTTTGCTGACTGGACATGTTGGGCCCTCCTGCCCTTAAATGCTTTGAAACTT >OriGene 3' read for NM_144665 unedited
GANGAGCCTGGGGNGGGGTCACAGGNATGCCACCCGGGTTCTGTTCAGGAAAAGCTATGA CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGCATTAA TACGCCAGATTCATGATTTATGATCAGTATCCAAAACTCCAACTACAAACAATGCAAAGT AGTGCTCCTCAGTATTATTTTTGCAATTGTTAGTAATGTTAAGCATCAAGGAAAATAAAA CACATCATTGCACATTACAGCCGCAAAAAACAAAAAACAAAAAAAAACAAACGGCTAAAC TTTGACACTAGAGAACTGAATAATTTTTGATGCTATATCACAAATAGCTTCAATGTTTAT CTTATGTGAAAGGTCTTTACACATGTATGACATTTTCCTTGGGTGATACTTCAGGTCAAA TGCCGAGTTATGGCACGAAGAGCATAAAGAAGTTCAGCTTGCATTCGTGCTTCCATTAAA AGTAGATTGACATGAACTTTTTCTGAGTGTTTGAACTGCCGCCAGTAACTATCATACATG CGTTTTGTAGTTCTCTCAGGATAGCAGGTCACTGTCTTAATGTAAACCTTCAGGCTTCTT TCAAGTAATTGATTAACTTCTCCATAATCATAGTCATCATACCTGATTCCAAACATACAG TGAACATAGTTAAATAAAGCTCTGCGCAGCATGGTTGTGTCAACATCCTCATGGGTGGCC ATAGTGTTATATGTGAGATTGTAGACCATCCGAAACTTTTCATCAAGAAGATGTCCAATG TCAGAATAAAGTCTGTTCACCAGGGGAGAACCCATGATTTTCCCAGGTTC |
| Restriction Sites | Please inquire |
| ACCN | NM_144665 |
| Insert Size | 2100 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_144665.2, NP_653266.2 |
| RefSeq Size | 1987 bp |
| RefSeq ORF | 1479 bp |
| Locus ID | 143686 |
| UniProt ID | P58005 |
| Domains | PA26 |
| Protein Pathways | p53 signaling pathway |
| Gene Summary | This gene encodes a member of the sestrin family of stress-induced proteins. The encoded protein reduces the levels of intracellular reactive oxygen species induced by activated Ras downstream of RAC-alpha serine/threonine-protein kinase (Akt) and FoxO transcription factor. The protein is required for normal regulation of blood glucose, insulin resistance and plays a role in lipid storage in obesity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Regulatory Effects of Sestrin 3 (SESN3) in BCR-ABL Expressing Cells
,Vakana, E;Arslan, AD;Szilard, A;Altman, JK;Platanias, LC;,
PLoS ONE e78780
,PubMed ID 24260131
[SESN3]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219818 | SESN3 (Myc-DDK-tagged)-Human sestrin 3 (SESN3) |
CNY 5,488.00 |
|
| RC219818L1 | Lenti ORF clone of Human sestrin 3 (SESN3), Myc-DDK-tagged |
CNY 6,180.00 |
|
| RC219818L2 | Lenti ORF clone of Human sestrin 3 (SESN3), mGFP tagged |
CNY 7,888.00 |
|
| RC219818L3 | Lenti ORF clone of Human sestrin 3 (SESN3), Myc-DDK-tagged |
CNY 6,180.00 |
|
| RC219818L4 | Lenti ORF clone of Human sestrin 3 (SESN3), mGFP tagged |
CNY 6,180.00 |
|
| RG219818 | SESN3 (tGFP-tagged) - Human sestrin 3 (SESN3) |
CNY 7,088.00 |
