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PDCD4 (NM_145341) Human Untagged Clone
CAT＃: SC313564
PDCD4 (untagged)-Human programmed cell death 4 (neoplastic transformation inhibitor) (PDCD4), transcript variant 2
CN¥ 7,320.00
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热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | H731 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_145341 edited
ATGACCAAATATCCTGATAACTTAAGTGACTCTCTCTTTTCCGGTGATGAAGAAAATGCT GGGACTGAGGAAGTAAAGAATGAAATAAATGGAAATTGGATTTCAGCATCCTCCATTAAC GAAGCTAGAATTAATGCCAAGGCAAAAAGGCGACTAAGGAAAAACTCATCCCGGGACTCT GGCAGAGGCGATTCGGTCAGCGACAGTGGGAGTGACGCCCTTAGAAGTGGATTAACTGTG CCAACCAGTCCAAAGGGAAGGTTGCTGGATAGGCGATCCAGATCTGGGAAAGGAAGGGGA CTACCAAAGAAAGGTGGTGCAGGAGGCAAAGGTGTCTGGGGTACACCTGGACAGGTGTAT GATGTGGAGGAGGTGGATGTGAAAGATCCTAACTATGATGATGACCAGGAGAACTGTGTT TATGAAACTGTAGTTTTGCCTTTGGATGAAAGGGCATTTGAGAAGACTTTAACACCAATC ATACAGGAATATTTTGAGCATGGAGATACTAATGAAGTTGCGGAAATGTTAAGAGATTTA AATCTTGGTGAAATGAAAAGTGGAGTACCAGTGTTGGCAGTATCCTTAGCATTGGAGGGG AAGGCTAGTCATAGAGAGATGACATCTAAGCTTCTTTCTGACCTTTGTGGGACAGTAATG AGCACAACTGATGTGGAAAAATCATTTGATAAATTGTTGAAAGATCTACCTGAATTAGCA CTGGATACTCCTAGAGCACCACAGTTGGTGGGCCAGTTTATTGCTAGAGCTGTTGGAGAT GGAATTTTATGTAATACCTATATTGATAGTTACAAAGGAACTGTAGATTGTGTGCAGGCT AGAGCTGCTCTGGATAAGGCTACCGTGCTTCTGAGTATGTCTAAAGGTGGAAAGCGTAAA GATAGTGTGTGGGGCTCTGGAGGTGGGCAGCAATCTGTCAATCACCTTGTTAAAGAGATT GATATGCTGCTGAAAGAATATTTACTCTCTGGAGACATATCTGAAGCTGAACATTGCCTT AAGGAACTGGAAGTACCTCATTTTCACCATGAGCTTGTATATGAAGCTATTATAATGGTT TTAGAGTCAACTGGAGAAAGTACATTTAAGATGATTTTGGATTTATTAAAGTCCCTTTGG AAGTCTTCTACCATTACTGTAGACCAAATGAAAAGAGGTTATGAGAGAATTTACAATGAA ATTCCGGACATTAATCTGGATGTCCCACATTCATACTCTGTGCTGGAGCGGTTTGTAGAA GAATGTTTTCAGGCTGGAATAATTTCCAAACAACTCAGAGATCTTTGTCCTTCAAGGGGC AGAAAGCGTTTTGTAAGCGAAGGAGATGGAGGTCGTCTTAAACCAGAGAGCTACTGA >OriGene 5' read for NM_145341 unedited
GAAAATCGTTCTTCAATTTGTNAATACCAACCTCACTATAGGGGCGGGCCGCGNATATTC GGCACGAGGGTTCGGGGCCGGCTGACCAGNAACCTGGGCGAGCAGCGGCGGGGGCCCGAG GGATTCTGAAGGAAGATTTCCATTAGGTAATTTGTTTAATCAGTGCAAGCGAAATTAAGG GAAAATGGATGTAGAAAATGAGCAGATACTGAATGTAAACCCTGCAGGGTATTTTCCCTA ATTCTCCATGGTGCTTCAATAGCATGTTATTATCATAAAAATGAACAGTTTTGTGGAATA GATGACCAAATATCCTGATAACTTAAGTGACTCTCTCTTTTCCGGTGATGAAGAAAATGC TGGGACTGAGGAAGTAAAGAATGAAATAAATGGAAATTGGATTTCAGCATCCTCCATTAA CGAAGCTAGAATTAATGCCAAGGCAAAAAGGCGACTAAGGAAAAACTCATCCCGGGACTC TGGCAGAGGCGATTCGGTCAGCGACAGTGGGAGTGACGCCCTTAGAAGTGGATTAACTGT GCCAACCAGTCCAAAGGGAAGGTTGCTGGATAGGCGATCCAGATCTGGGAAAGGAAGGGG ACTACCAAAGAAAGGTGGTGCAGGAGGCAAAGGTGTCTGGGGTACACCTGGACAGGTGTA TGATTGGAAGAGTTGGGGATTTTTNNAAAGATTCTAACTATGATGATGACCANGNAGAAC TGTGTTTATGAAACTGGTATTTTGCCTTTGGATGAAAGGGCATTTGAAAAGACTTTAACA CCCATCCTACAGGAATTTTTTGAGCATGGAAATACTAATGGAGTTGCGGAAATGTTAAGA AGATTTAATCTTGGTGAAATTGAAAGTGGGATTCCCGTGTTTGGCAGTTTCCTTAACTTG GAGGGGGAAGGCTAGTTCATAAAAAA >Forward primer walk for NM_145341 unedited
ATCTTTAGAGCATGGAGAACTAATGAAGTTGCGGATATGTTAAGAGATTTAAATCTTGGT GAAATGAAAAGTGGAGTACCAGTGTTGGCAGTATCCTTAGCATTGGAGGGGAAGGCTAGT CATAGAGAGATGACATCTAAGCTTCTTTCTGACCTTTGTGGGACAGTAATGAGCACAACT GATGTGGAAAAATCATTTGATAAATTGTTGAAAGATCTACCTGAATTAGCACTGGATACT CCTAGAGCACCACAGTTGGTGGGCCAGTTTATTGCTAGAGCTGTTGGAGATGGAATTTTA TGTAATACCTATATTGATAGTTACAAAGGAACTGTAGATTGTGTGCAGGCTAGAGCTGCT CTGGATAAGGCTACCGTGCTTCTGAGTATGTCTAAAGGTGGAAAGCGTAAAGATAGTGTG TGGGGCTCTGGAGGTGGGCAGCAATCTGTCAATCACCTTGTTAAAGAGATTGATATGCTG CTGAAAGAATATTTACTCTCTGGAGACATATCTGAAGCTGAACATTGCCTTAAGGAACTG GAAGTACCTCATTTTCACCATGAGCTTGTATATGAAGCTATTATAATGGTTTTAGAGTCA ACTGGAGAAAGTACATTTAAGATGATTTTGGATTTATTAAAGTCCCTTTGGAAGTCTTCT ACCATTACTGTAGACCAAATGAAAAGAGGTTATGAGAGAATTTACAATGAAATTCCGGAC ATTAATCTGGATGTCCCACATTCATACTCTGTGCTGGAGCGGTTTGTAGAAGAATGTTTT CAGGCTGGAATAATTTCCAAACAACTCAGAGATCTTTGTCCTTCATGGGGCAGAAAGCGT TTTGTAGCGAAGAGATGGAGTCGTCTTAACCCAGGAGAGCTACTGAATATTAAGAACTCT TGCAGTCTTAGAATGTTATAAAAAATATATATTCTGAATTGTAAGAAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_145341 |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to differ from the protein associated to this reference by a single amino acid. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_145341.2, NP_663314.1 |
| RefSeq Size | 3675 bp |
| RefSeq ORF | 1377 bp |
| Locus ID | 27250 |
| UniProt ID | Q53EL6 |
| Domains | MA3 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010] Transcript Variant: This variant (2) includes an alternate exon in the 5' coding region and utilizes a downstream start codon, compared to variant 1. The resulting protein (isoform 2) has a shorter and distinct N-terminus, compared to isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222260 | PDCD4 (Myc-DDK-tagged)-Human programmed cell death 4 (neoplastic transformation inhibitor) (PDCD4), transcript variant 2 |
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| RC222260L3 | Lenti-ORF clone of PDCD4 (Myc-DDK-tagged)-Human programmed cell death 4 (neoplastic transformation inhibitor) (PDCD4), transcript variant 2 |
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| RC222260L4 | Lenti-ORF clone of PDCD4 (mGFP-tagged)-Human programmed cell death 4 (neoplastic transformation inhibitor) (PDCD4), transcript variant 2 |
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| RG222260 | PDCD4 (tGFP-tagged) - Human programmed cell death 4 (neoplastic transformation inhibitor) (PDCD4), transcript variant 2 |
CN¥ 5,040.00 |
