SMPDL3A (BC018999) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	0610010C24Rik; acid sphingomyelinase-like phosphodiesterase 3a; AI529588; ASM-like phosphodiesterase 3a; ASM3A; ASML3; ASML3A; OTTMUSP00000028536; sphingomyelin phosphodiesterase, acid-like 3A; yR36GH4.1
Vector	pCMV6 series
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC018999, the custom clone sequence may differ by one or more nucleotides ATGGCGCTGGTGCGCGCACTCGTCTGCTGCCTGCTGACTGCCTGGCACTGCCGCTCCGGC CTCGGGCTGCCCGTGGCGCCCGCAGGCGGCAGGAATCCTCCTCCGGCGATAGGACAGTTT TGGCATGTGACTGACTTACACTTAGACCCTACTTACCACATCACAGATGACCACACAAAA GTGTGTGCTTCATCTAAAGGTGCAAATGCCTCCAACCCTGGCCCTTTTGGAGATGTTCTG TGTGATTCTCCATATCAACTTATTTTGTCAGCATTTGATTTTATTAAAAATTCTGGACAA GAAGCATCTTTCATGATATGGACAGGGGATAGCCCACCTCATGTTCCTGTACCTGAACTC TCAACAGACACTGTTATAAATGTGATCACTAATATGACAACCACCATCCAGAGTCTCTTT CCAAATCTCCAGGTTTTCCCTGCGCTGGGTAATCATGACTATTGGCCACAGGATCAACTG CCTGTAGTCACCAGTAAAGTGTACAATGCAGTAGCAAACCTCTGGAAACCATGGCTAGAT GAAGAAGCTATTAGTACTTTAAGGAAAGGTGGTTTTTATTCACAGAAAGTTACAACTAAT CCAAACCTTAGGATCATCAGTCTAAACACAAACTTGTACTACGGCCCAAATATAATGACA CTGAACAAGACTGACCCAGCCAACCAGTTTGAATGGCTAGAAAGTACATTGAACAACTCT CAGCAGAATAAGGAGAAGGTGTATATCATAGCACATGTTCCAGTGGGGTATCTGCCATCT TCACAGAACATCACAGCAATGAGAGAATACTATAATGAGAAATTGATAGATATTTTTCAA AAATACAGTGATGTCATTGCAGGACAATTTTATGGACACACTCACAGAGACAGCATTATG GTTCTTTCAGATAAAAAAGGAAGTCCAGTAAATTCTTTGTTTGTGGCTCCTGCTGTTACA CCAGTGAAGAGTGTTTTAGAAAAACAGACCAACAATCCTGGTATCAGACTGTTTCAGTAT GATCCTCGTGATTATAAATTATTGGATATGTTGCAGTATTACTTGAATCTGACAGAGGCG AATCTAAAGGGAGAGTCCATCTGGAAGCTGGAGTATATCCTGACCCAGACCTACGACATT GAAGATTTGCAGCCGGAAAGTTTATATGGATTAGCTAAACAATTTACAATCCTAGACAGT AAGCAGTTTATAAAATACTACAATTACTTCTTTGTGAGTTATGACAGCAGTGTAACATGT GATAAGACATGTAAGGCCTTTCAGATTTGTGCAATTATGAATCTTGATAATATTTCCTAT GCAGATTGCCTCAAACAGCTTTATATAAAGCACAATTAC
Restriction Sites	Please inquire
ACCN	BC018999
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC018999.2, AAH18999.1
RefSeq Size	1759 bp
RefSeq ORF	1362 bp
Locus ID	10924
Domains	Metallophos
Protein Families	Secreted Protein
Gene Summary	Has in vitro nucleotide phosphodiesterase activity with nucleoside triphosphates, such as ATP (PubMed:25288789, PubMed:26783088). Has in vitro activity with p-nitrophenyl-TMP (PubMed:25288789). Has lower activity with nucleoside diphosphates, and no activity with nucleoside monophosphates (PubMed:25288789, PubMed:26783088). Has in vitro activity with CDP-choline, giving rise to CMP and phosphocholine. Has in vitro activity with CDP-ethanolamine (PubMed:26783088). Does not have sphingomyelin phosphodiesterase activity (PubMed:25288789, PubMed:26783088).[UniProtKB/Swiss-Prot Function]

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