United States
Germany
Japan
United Kingdom
China
ENGASE (NM_001042573) Human Untagged Clone
CAT＃: SC311407
ENGASE (untagged)-Human endo-beta-N-acetylglucosaminidase (ENGASE)
CNY 14,250.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC311407 representing NM_001042573.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGGCCGCGGCGGTGACGGTCACCCGGTCGGCTACACGGCGGCGGCGGCGGCAGCTGCAGGGGCTG GCGGCCCCGGAGGCGGGGACGCAGGAGGAGCAGGAGGATCAGGAGCCGCGGCCGCGGCGGCGGCGGCCG GGAAGGAGCATCAAAGATGAAGAAGAAGAGACAGTCTTTCGAGAGGTGGTCAGTTTTTCCCCGGACCCC CTGCCAGTTAGATATTATGACAAGGACACCACCAAACCAATCAGCTTTTACTTGTCTTCGCTGGAGGAG CTCTTGGCGTGGAAGCCCCGCTTGGAGGATGGCTTTAATGTGGCCCTGGAGCCCCTGGCGTGTCGCCAG CCCCCTCTGAGCAGCCAGAGGCCCCGGACTTTGTTGTGTCATGACATGATGGGCGGGTACCTGGATGAC AGGTTCATTCAGGGCTCGGTGGTGCAGACTCCCTATGCTTTCTACCACTGGCAGTGCATCGACGTCTTT GTGTACTTCAGCCACCACACCGTCACCATTCCCCCAGTGGGCTGGACCAACACTGCCCACAGGCATGGG GTCTGCGTGCTGGGGACTTTCATCACGGAGTGGAATGAAGGGGGAAGGCTCTGTGAAGCCTTCCTGGCC GGGGATGAGCGCTCGTACCAGGCAGTGGCTGACCGGCTGGTCCAGATCACTCAGTTTTTTCGTTTTGAT GGCTGGCTGATCAACATCGAGAACTCGCTGAGTCTGGCCGCTGTGGGGAACATGCCTCCTTTCCTGCGG TACCTCACCACACAGCTGCACCGGCAGGTCCCAGGGGGCCTGGTGCTCTGGTATGACAGCGTGGTGCAA AGTGGGCAGCTCAAATGGCAAGACGAACTCAACCAGCACAACAGGGTCTTCTTTGATTCCTGCGACGGC TTCTTCACTAACTATAACTGGCGGGAGGAGCACTTGGAGCGGATGCTGGGGCAGGCTGGGGAGCGCCGG GCTGATGTGTACGTGGGCGTGGATGTGTTTGCTCGAGGGAACGTGGTCGGAGGCCGATTCGACACAGAC AAGTCGTTGGAGCTGATCCGAAAGCATGGCTTCTCCGTGGCTTTGTTTGCCCCCGGCTGGGTGTATGAG TGTCTGGAGAAGAAGGATTTCTTCCAGAACCAGGACAAGTTCTGGGGCCGACTGGAGCGTTATCTGCCC ACACATAGCATCTGCTCCTTGCCTTTCGTCACGTCCTTCTGCCTGGGCATGGGTGCACGGAGGGTCTGC TATGGCCAGGAAGAGGCGGTAGGGCCCTGGTACCACCTGAGCGCCCAGGAGATCCAGCCCTTGTTTGGA GAACACAGGCTGGGAGGGGATGGCCGGGGCTGGGTGAGGACGCACTGCTGCCTGGAGGATGCCTGGCAC GGAGGCAGCTCCCTGCTCGTCCGGGGTGTGATCCCACCGGAGGTTGGAAATGTGGCTGTGAGGTTATTT TCCCTGCAGGCCCCAGTGCCACCCAAGATTTACCTGTCCATGGTGTATAAGCTTGAGGGGCCCACGGAC GTCACAGTTGCTTTGGAGCTGACCACAGGGGATGCCGGCAGCTGCCACATCGGTGGCATCTCAGTGTTG AACGCAGAAACAAGCTCAAGACACAGCCTCCGACCCCTCCGGGTGCCCCCCACCAAGCTGGCCAGATGG GTGGGCCGCTGCGGCCGGCAGCTGAGTGGGGGCTGGGTCCAGCACTGCTACGAGGTGAGCCTGCGTGGG TGCCTGCTGCTAGACCTCCTCGTTTGCTTCTCACGGCCGCCGGGTAGTCGGGAGGAGGAGAGCTTCACC TGTCGGCTTGGAGAGATCCAGGTGGTGGACGCTGCCAGCCTGCTGGCCCCTCTGCCCCAGGTGCAGGCC GTCACCATCTCTCACATCCGCTGGCAGCCATCCGCCTCTGAGCGGGAGGGGCCCCCTGCTCTGCTCCAG CTCAGCTGCACCCTGCACTGGTCCTTCCTCCTCTCACAAGTCCGTTGCTTCCGAATCCACTGCTGGGGA GGGATGAGTGATGACTCTCCGGGCAGGGAGCTGCCGAGGCCAGAGATGCCCATGTTCCTGGGGTTGGCT TTTGCCACCCAGTACCGGATAGTGGACCTGCTGGTGGAAGCCGCCGGGCCCGGCCAGGATCGTCGCATG GAATTTCTGGTGGAGCCTGTCCCCAAGGAAGGGTTCCGGGTACCTCAGGCCGAGTGGGGCAGGGCAGTT CTGCTTTATTCAGCCCCTGCATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001042573 |
| Insert Size | 2232 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001042573.2 |
| RefSeq Size | 4494 bp |
| RefSeq ORF | 2232 bp |
| Locus ID | 64772 |
| UniProt ID | Q8NFI3 |
| Protein Pathways | Other glycan degradation |
| MW | 84 kDa |
| Gene Summary | This gene encodes a cytosolic enzyme which catalyzes the hydrolysis of peptides and proteins with mannose modifications to produce free oligosaccharides. [provided by RefSeq, Feb 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211494 | ENGASE (Myc-DDK-tagged)-Human endo-beta-N-acetylglucosaminidase (ENGASE) |
CNY 5,440.00 |
|
| RC211494L3 | Lenti ORF clone of Human endo-beta-N-acetylglucosaminidase (ENGASE), Myc-DDK-tagged |
CNY 9,690.00 |
|
| RC211494L4 | Lenti ORF clone of Human endo-beta-N-acetylglucosaminidase (ENGASE), mGFP tagged |
CNY 9,690.00 |
|
| RG211494 | ENGASE (tGFP-tagged) - Human endo-beta-N-acetylglucosaminidase (ENGASE) |
CNY 8,550.00 |
