United States
Germany
Japan
United Kingdom
China
KCNT1 (NM_020822) Human Untagged Clone
CAT＃: SC311132
KCNT1 (untagged)-Human potassium channel, subfamily T, member 1 (KCNT1)
CNY 13,128.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | bA100C15.2; DEE14; EIEE14; ENFL5; KCa4.1; SLACK; Slo2.2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>NCBI ORF sequence for NM_020822, the custom clone sequence may differ by one or more nucleotides
ATGCCACTCCCTGACGGGGCGCGGACCCCGGGGGGCGTCTGCCGGGAGGCGCGCGGCGGG GGCTACACCAACCGGACCTTCGAGTTTGACGACGGCCAATGCGCCCCCAGGCGGCCCTGC GCGGGGGACGGCGCGCTCCTGGACACCGCCGGCTTCAAGATGAGCGACCTGGACTCCGAG GTGCTGCCCTTGCCGCCGCGCTACCGCTTCCGGGACCTGCTGCTGGGCGACCCGTCCTTC CAGAACGACGACAGGGTCCAGGTGGAGTTCTACGTCAACGAGAACACCTTCAAGGAGCGG CTCAAGCTGTTCTTCATCAAAAACCAAAGATCGAGCCTGAGGATCCGGCTGTTCAACTTC TCCCTGAAGCTGCTCACCTGCCTGCTCTACATTGTGCGCGTCCTGCTCGATGACCCGGCC CTGGGCATCGGATGCTGGGGCTGCCCAAAGCAGAACTACTCCTTCAATGACTCGTCCTCC GAGATCAACTGGGCTCCTATTCTGTGGGTGGAGAGAAAGATGACACTGTGGGCGATCCAG GTCATCGTGGCCATAATAAGCTTCCTGGAGACGATGCTTCTCATCTACCTCAGCTACAAA GGCAACATCTGGGAGCAGATCTTCCGCGTGTCCTTCGTCCTGGAGATGATCAACACTCTG CCCTTCATCATCACGATCTTCTGGCCGCCGCTGCGGAACCTGTTCATCCCCGTCTTTCTG AACTGCTGGCTGGCCAAGCACGCGCTGGAAAACATGATTAATGACTTCCACCGTGCCATC CTGCGGACACAGTCAGCCATGTTCAACCAGGTCCTCATCCTCTTCTGCACCCTGCTGTGC CTCGTTTTCACGGGGACCTGCGGCATCCAGCACCTGGAGCGGGCGGGCGAGAACCTGTCC CTCCTGACCTCCTTCTACTTCTGCATCGTCACCTTCTCCACCGTGGGCTACGGTGACGTC ACGCCCAAGATCTGGCCATCGCAGCTGCTGGTGGTCATCATGATCTGCGTGGCCCTCGTG GTGCTCCCACTGCAGTTCGAGGAGCTCGTCTACCTCTGGATGGAGCGGCAGAAGTCAGGG GGCAACTACAGCCGCCACCGTGCGCAGACGGAGAAGCACGTGGTCCTGTGTGTCAGCTCC CTCAAGATCGACCTTCTCATGGACTTCCTGAACGAGTTCTACGCCCACCCCCGGCTCCAG GACTATTACGTGGTCATCCTGTGCCCCACGGAGATGGATGTCCAGGTGCGCAGAGTCCTG CAGATCCCTCTGTGGTCCCAGCGGGTCATCTACCTCCAGGGCTCTGCACTCAAAGACCAG GACCTCATGCGAGCCAAGATGGACAATGGGGAGGCCTGCTTCATCCTCAGCAGCAGGAAC GAGGTGGACCGCACGGCTGCAGACCACCAGACCATCCTGCGCGCCTGGGCCGTGAAGGAC TTCGCCCCCAACTGCCCCCTCTACGTCCAGATCCTCAAACCTGAAAACAAGTTTCACGTC AAGTTTGCTGACCACGTGGTGTGTGAGGAGGAGTGCAAGTACGCCATGCTGGCGCTGAAC TGCATCTGCCCGGCGACCTCCACCCTCATCACCCTGCTGGTGCACACGTCCCGCGGCCAG GAGGGACAGGAGTCTCCGGAGCAGTGGCAGCGCATGTATGGGCGCTGCTCCGGCAACGAG GTGTACCACATCCGCATGGGTGACAGCAAGTTCTTCCGCGAGTACGAGGGCAAGAGCTTC ACCTACGCGGCCTTCCACGCCCACAAGAAGTATGGCGTGTGCCTCATCGGGCTGAAGCGG GAGGACAACAAGAGCATCCTGCTGAACCCGGGGCCCCGGCACATCCTGGCCGCCTCTGAC ACCTGCTTCTACATCAACATCACCAAGGAGGAGAACTCGGCCTTCATCTTCAAGCAGGAG GAGAAGCGGAAGAAGAGGGCCTTCTCGGGGCAGGGGCTGCACGAGGGTCCGGCCCGCCTG CCCGTGCACAGCATCATCGCCTCCATGGGGACAGTGGCCATGGACCTGCAGGGCACAGAG CACCGGCCTACGCAGAGCGGCGGTGGGGGCGGGGGCAGCAAGCTGGCACTGCCCACGGAG AACGGCTCGGGCAGCCGGCGGCCCAGCATCGCGCCCGTCCTGGAACTGGCCGACAGCTCA GCCCTGCTGCCCTGCGACCTGCTGAGCGACCAGTCGGAGGATGAGGTGACGCCGTCGGAC GACGAGGGGCTCTCCGTGGTAGAGTATGTGAAGGGCTACCCTCCCAACTCGCCCTACATC GGCAGCTCCCCAACCCTGTGCCACCTCCTGCCTGTGAAAGCCCCCTTCTGCTGCCTGCGG CTGGACAAGGGCTGCAAGCACAACAGCTATGAAGACGCCAAGGCCTACGGGTTCAAGAAC AAGCTGATCATCGTCTCGGCAGAGACGGCCGGCAATGGGCTGTACAACTTCATCGTGCCA CTGCGGGCCTACTACAGATCCCGCAAGGAGCTGAACCCCATCGTGCTGCTGCTGGACAAC AAGCCCGACCACCACTTCCTGGAAGCCATCTGCTGCTTCCCCATGGTCTACTACATGGAG GGCTCTGTGGACAACCTGGACAGCCTGCTGCAGTGTGGCATCATCTATGCGGACAACCTG GTGGTGGTGGACAAGGAGAGCACCATGAGCGCCGAGGAGGACTACATGGCGGACGCCAAG ACCATCGTCAACGTGCAGACCATGTTCCGGCTCTTCCCCAGCCTCAGCATCACCACGGAG CTCACCCACCCTTCCAACATGCGCTTCATGCAGTTCCGCGCCAAGGACAGCTACTCTCTG GCTCTTTCCAAACTAGAAAAGAGGGAGCGAGAGAATGGCTCCAACCTGGCCTTCATGTTC CGCCTGCCGTTCGCCGCCGGCCGCGTCTTCAGCATCAGCATGTTGGACACACTGCTCTAC CAGTCCTTCGTGAAGGACTACATGATCACCATCACCCGGCTGCTGCTGGGCCTGGACACC ACGCCGGGCTCGGGGTACCTCTGTGCCATGAAAATCACCGAGGGCGACCTGTGGATCCGC ACGTACGGCCGCCTCTTCCAGAAGCTCTGCTCCTCCAGCGCCGAGATCCCCATTGGCATC TACCGGACAGAGAGCCACGTCTTCTCCACCTCGGAGCCCCACGACCTCAGAGCCCAGTCC CAGATCTCGGTGAACGTGGAGGACTGTGAGGACACACGGGAAGTGAAGGGGCCCTGGGGC TCCCGCGCTGGCACCGGAGGCAGCTCCCAGGGCCGCCACACGGGCGGCGGTGACCCCGCA GAGCACCCACTGCTACGGCGCAAGAGCCTGCAGTGGGCCCGGAGGCTGAGCCGCAAGGCG CCCAAGCAGGCAGGCCGGGCGGCGGCCGCGGAGTGGATCAGCCAGCAGCGCCTCAGCCTG TACCGGCGCTCTGAGCGCCAGGAGCTCTCCGAGCTGGTGAAGAACCGCATGAAGCACCTG GGGCTGCCCACCACCGGCTACGACGAGATGAACGACCACCAGAACACCCTCTCCTACGTC CTCATCAACCCTCCGCCCGACACGAGGCTGGAGCCCAGTGACATTGTCTATCTCATCCGC TCCGACCCCCTGGCTCACGTGGCCAGCAGCTCCCAGAGCCGGAAGAGCAGCTGCAGCCAC AAGCTGTCGTCCTGCAACCCCGAGACTCGCGACGAGACACAGCTCTGA |
| Restriction Sites | Please inquire |
| ACCN | NM_020822 |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020822.1, NP_065873.1 |
| RefSeq Size | 4823 bp |
| RefSeq ORF | 3771 bp |
| Locus ID | 57582 |
| UniProt ID | Q5JUK3 |
| Protein Families | Druggable Genome, Ion Channels: Potassium, Transmembrane |
| Gene Summary | Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a sodium-activated potassium channel subunit which is thought to function in ion conductance and developmental signaling pathways. Mutations in this gene cause the early-onset epileptic disorders, malignant migrating partial seizures of infancy and autosomal dominant nocturnal frontal lobe epilepsy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012] Transcript Variant: This variant (1) encodes the longer isoform (1). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Functional Effects of Epilepsy Associated KCNT1 Mutations Suggest Pathogenesis via Aberrant Inhibitory Neuronal Activity
,null,
International Journal of Molecular Sciences
,PubMed ID 36499459
[KCNT1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214820 | KCNT1 (Myc-DDK-tagged)-Human potassium channel, subfamily T, member 1 (KCNT1) |
CNY 13,120.00 |
|
| RC214820L1 | Lenti-ORF clone of KCNT1 (Myc-DDK-tagged)-Human potassium channel, subfamily T, member 1 (KCNT1) |
CNY 15,520.00 |
|
| RC214820L2 | Lenti-ORF clone of KCNT1 (mGFP-tagged)-Human potassium channel, subfamily T, member 1 (KCNT1) |
CNY 15,520.00 |
|
| RC214820L3 | Lenti-ORF clone of KCNT1 (Myc-DDK-tagged)-Human potassium channel, subfamily T, member 1 (KCNT1) |
CNY 11,500.00 |
|
| RC214820L4 | Lenti-ORF clone of KCNT1 (mGFP-tagged)-Human potassium channel, subfamily T, member 1 (KCNT1) |
CNY 11,500.00 |
|
| RG214820 | KCNT1 (tGFP-tagged) - Human potassium channel, subfamily T, member 1 (KCNT1) |
CNY 14,720.00 |
