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Osteopontin (SPP1) (NM_001040058) Human Untagged Clone
CAT＃: SC311077
SPP1 (untagged)-Human secreted phosphoprotein 1 (SPP1), transcript variant 1
CNY 2,400.00
CNY 6,270.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BNSP; BSPI; ETA-1; OPN |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_001040058 edited
GAATTCGGCACGAGGCAAACGCCGACCAAGGAAAACTCACTACCATGAGAATTGCAGTGA TTTGCTTTTGCCTCCTAGGCATCACCTGTGCCATACCAGTTAAACAGGCTGATTCTGGAA GTTCTGAGGAAAAGCAGCTTTACAACAAATACCCAGATGCTGTGGCCACATGGCTAAACC CTGACCCATCTCAGAAGCAGAATCTCCTAGCCCCACAGAATGCTGTGTCCTCTGAAGAAA CCAATGACTTTAAACAAGAGACCCTTCCAAGTAAGTCCAACGAAAGCCATGACCACATGG ATGATATGGATGATGAAGATGATGATGACCATGTGGACAGCCAGGACTCCATTGACTCGA ACGACTCTGATGATGTAGATGACACTGATGATTCTCACCAGTCTGATGAGTCTCACCATT CTGATGAATCTGATGAACTGGTCACTGATTTTCCCACGGACCTGCCAGCAACCGAAGTTT TCACTCCAGTTGTCCCCACAGTAGACACATATGATGGCCGAGGTGATAGTGTGGTTTATG GACTGAGGTCAAAATCTAAGAAGTTTCGCAGACCTGACATCCAGTACCCTGATGCTACAG ACGAGGACATCACCTCACACATGGAAAGCGAGGAGTTGAATGGTGCATACAAGGCCATCC CCGTTGCCCAGGACCTGAACGCGCCTTCTGATTGGGACAGCCGTGGGAAGGACAGTTATG AAACGAGTCAGCTGGATGACCAGAGTGCTGAAACCCACAGCCACAAGCAGTCCAGATTAT ATAAGCGGAAAGCCAATGATGAGAGCAATGAGCATTCCGATGTGATTGATAGTCAGGAAC TTTCCAAAGTCAGCCGTGAATTCCACAGCCATGAATTTCACAGCCATGAAGATATGCTGG TTGTAGACCCCAAAAGTAAGGAAGAAGATAAACACCTGAAATTTCGTATTTCTCATGAAT TAGATAGTGCATCTTCTGAGGTCAATTAAAAGGAGAAAAAATACAATTTCTCACTTTGCA TTTAGTCAAAAGAAAAAATGCTTTATAGCAAAATGAAAGAGAACATGAAATGCTTCTTTC TCAGTTTATTGGTTGAATGTGTATCTATTTGAGTCTGGAAATAACTAATGTGTTTGATAA TTAGTTTAGTTTGTGGCTTCATGGAAACTCCCTGTAAACTAAAAGCTTCAGGGTTATGTC TATGTTCATTCTATAGAAGAAATGCAAACTATCACTGTATTTTAATATTTGTTATTCTCT CATGAATAGAAATTTATGTAGAAGCAAACAAAATACTTTTACCCACTTAAAAAGAGAATA TAACATTTTATGTCACTATAATCTTTTGTTTTTTAAGTTAGTGTATATTTTGTTGTGATT ATCTTTTTGTGGTGTGAATAAATCTTTTATCTTGAATGTAAAAAAAAAAAAAAAAAACTC GAC >OriGene 5' read for NM_001040058 unedited
TACGACTCACTATAGGCGGCCGCGAATTCGGCACGAGGGGCAAACGCCGACCAAGGGAAA ACTCACTACCATGAGAATTGCAGTGATTTGCTTTTGCCTCCTAGGCATCACCTGTGCCAT ACCAGTTAAACAGGCTGATTCTGGAAGTTCTGAGGAAAAGCAGCTTTACAACAAATACCC AGATGCTGTGGCCACATGGCTAAACCCTGACCCATCTCAGAAGCAGAATCTCCTAGCCCC ACAGAATGCTGTGTCCTCTGAAGAAACCAATGACTTTAAACAAGAGACCCTTCCAAGTAA GTCCAACGAAAGCCATGACCACATGGATGATATGGATGATGAAGATGATGATGACCATGT GGACAGCCAGGACTCCATTGACTCGAACGACTCTGATGATGTAGATGACACTGATGATTC TCACCAGTCTGATGAGTCTCACCATTCTGATGAATCTGATGAACTGGTCACTGATTTTCC CACGGACCTGCCAGCAACCGAAGTTTTCACTCCAGTTGTCCCCACAGTAGACACATATGA TGGCCGAGGTGATAGTGTGGTTTATGGACTGAGGTCAAAATCTAAGAAGTTTCGCAGACC TGACATCCAGTACCCTGATGCTACAGACGAGGACATCACCTCACACATGGAAAGCGAGGA GTTGAATGGTGCTACAAGGCCATCCCCGTTGCCCAGGACCTGAACGCGCCTTCTGATTGG GACAGCCGTGGGAAGGACAGTTATGAAACGAGTCAGCTGGATGACCAGAGTGCTGAAACC CACAGNCACAAGCAGTCCAGATTATATAAGCGGAAAGCCATGATGAGAGCNATGAGCATT NCGATGTAATTGATATCAGGAACTTTNCAAGTTCAGCGTGATTNCNCAGNCATGAATTNA CAGCATGAGAATTGCTGGTTGGAACCCCAAGTAGGAGAAGGAAACACTGAAAATTCGATC TATGAATAAAAGGCTTTCTGGGGCATAAAGGAAAATCAATCTCCTTGGCTATCAAGAAAA GCTTTCAGGA >OriGene 3' read for NM_001040058 unedited
GGCTTTAACTATGNNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTAC ATTCAAGATAAAAGATTTATTCACACCACAAAAAGATAATCACAACAAAATATACACTAA CTTAAAAAACAAAAGATTATAGTGACATAAAATGTTATATTCTCTTTTTAAGTGGGTAAA AGTATTTTGTTTGCTTCTACATAAATTTCTATTCATGAGAGAATAACAAATATTAAAATA CAGTGATAGTTTGCATTTCTTCTATAGAATGAACATAGACATAACCCTGAAGCTTTTAGT TTACAGGGAGTTTCCATGAAGCCACAAACTAAACTAATTATCAAACACATTAGTTATTTC CAGACTCAAATAGATACACATTCAACCAATAAACTGAGAAAGAAGCATTTCATGTTCTCT TTCATTTTGCTATAAAGCATTTTTTCTTTTGACTAAATGCAAAGTGAGAAATTGTATTTT TTCTCCTTTTAATTGACCTCAGAAGATGCACTATCTAATTCATGAGAAATACGAAATTTC AGGTGTTTATCTTCTTCCTTACTTTTGGGGTCTACAACCAGCATATCTTCATGGCTGTGA AATTCATGGCTGTGGAATTCACGGCTGACTTTGGAAAGTTCCTGACTATCAATCACATCG GAATGCTCATTGCTCTCATCATTGGCTTTCCGCTTATATAATCTGGACTGCTTGTGGCTG TGGGTTTCAGCACTCTGGTCATCCAGCTGACTCGTTTCATAACTGTCCTTCCCACGGCTG TCCCAATCAGAAGGCGCGTTCAGGTCCTGGNGCACGGGGATGGCCTTGTATGCACCATTC AACTCCTCGCTTTCCTGTGTGAGGNGATGTCCTCGCCTGTAGCATCAGGGTACTGGATGT CAGGTCTGCC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001040058 |
| Insert Size | 1400 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001040058.1, NP_001035147.1 |
| RefSeq Size | 1641 bp |
| RefSeq ORF | 945 bp |
| Locus ID | 6696 |
| UniProt ID | P10451 |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | ECM-receptor interaction, Focal adhesion, Toll-like receptor signaling pathway |
| Gene Summary | The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] Transcript Variant: This variant (1) differs in the 5' UTR and coding sequence compared to variant 5. The resulting isoform (OPN-a, as described in PubMed: 7837791) has a shorter and distinct N-terminus compared to isoform e. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Lung cancer osteopontin isoforms exhibit angiogenic functional heterogeneity
,Justin D. Blasberg, Chandra M. Goparaju, Harvey I. Pass, and Jessica S. Donington,
J. Thorac. Cardiovasc. Surg., Jun 2010; 139: 1587 - 1593
[SPP1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204803 | SPP1 (Myc-DDK-tagged)-Human secreted phosphoprotein 1 (SPP1), transcript variant 1 |
CNY 3,600.00 |
|
| RC204803L1 | Lenti ORF clone of Human secreted phosphoprotein 1 (SPP1), transcript variant 1, Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC204803L2 | Lenti ORF clone of Human secreted phosphoprotein 1 (SPP1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC204803L3 | Lenti ORF clone of Human secreted phosphoprotein 1 (SPP1), transcript variant 1, Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC204803L4 | Lenti ORF clone of Human secreted phosphoprotein 1 (SPP1), transcript variant 1, mGFP tagged |
CNY 6,000.00 |
|
| RG204803 | SPP1 (tGFP-tagged) - Human secreted phosphoprotein 1 (SPP1), transcript variant 1 |
CNY 5,200.00 |
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