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DCAMKL2 (DCLK2) (NM_001040260) Human Untagged Clone
CAT＃: SC310962
DCLK2 (untagged)-Human doublecortin-like kinase 2 (DCLK2), transcript variant 1
CNY 14,820.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CL2; CLICK-II; CLICK2; CLIK2; DCAMKL2; DCDC3; DCDC3B; DCK2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC310962 representing NM_001040260.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCAGCACCAGGAGTATCGAGCTGGAGCACTTTGAGGAACGGGACAAAAGGCCGCGGCCGGGGTCG CGGAGAGGGGCCCCCAGCTCCTCCGGGGGCAGCAGCAGCTCGGGCCCCAAGGGGAACGGGCTCATCCCC AGTCCGGCGCACAGTGCCCACTGCAGCTTCTACCGCACGCGGACCCTGCAGGCCCTCAGCTCGGAGAAG AAGGCCAAGAAGGCGCGCTTCTACCGGAACGGGGACCGCTACTTCAAGGGCCTGGTGTTTGCCATCTCC AGCGACCGCTTCCGGTCCTTCGATGCGCTCCTCATAGAGCTCACCCGCTCCCTGTCGGACAACGTGAAC CTGCCCCAGGGTGTCCGCACTATCTACACCATCGACGGCAGCCGGAAGGTCACCAGCCTGGACGAGCTG CTGGAAGGTGAGAGTTACGTGTGTGCATCCAATGAACCATTTCGTAAAGTCGATTACACCAAAAATATT AATCCAAACTGGTCTGTGAACATCAAGGGTGGGACATCCCGAGCGCTGGCTGCTGCCTCCTCTGTGAAA AGTGAAGTAAAAGAAAGTAAAGATTTCATCAAACCCAAGTTAGTGACTGTGATTCGAAGTGGAGTGAAG CCTAGAAAAGCCGTGCGGATCCTTCTGAATAAAAAGACTGCTCATTCCTTTGAACAAGTCTTAACAGAT ATCACCGAAGCCATTAAACTAGACTCAGGAGTCGTCAAGAGGCTCTGCACCCTGGATGGAAAGCAGGTT ACTTGTCTGCAAGACTTTTTTGGTGATGACGATGTTTTTATTGCATGTGGACCAGAAAAATTTCGTTAT GCCCAAGATGACTTTGTCCTGGATCATAGTGAATGTCGTGTCCTGAAGTCATCTTATTCTCGATCCTCA GCTGTTAAGTATTCTGGATCCAAAAGCCCTGGGCCCTCTCGACGCAGCAAATCACCAGCTTCAGTTAAT GGAACTCCCAGCAGCCAACTTTCTACTCCTAAATCTACGAAATCCTCCAGTTCCTCTCCAACTAGTCCA GGAAGTTTCAGAGGATTAAAGCAGATTTCTGCTCATGGCAGATCTTCTTCCAATGTAAACGGTGGACCT GAGCTTGACCGTTGCATAAGTCCTGAAGGTGTGAATGGAAACAGATGCTCTGAATCATCAACTCTTCTT GAGAAATACAAAATTGGAAAGGTCATTGGTGATGGCAATTTTGCAGTAGTCAAAGAGTGTATAGACAGG TCCACTGGAAAGGAGTTTGCCCTAAAGATTATAGACAAAGCCAAATGTTGTGGAAAGGAACACCTGATT GAGAATGAAGTGTCAATACTGCGCCGAGTGAAACATCCCAATATCATTATGCTGGTCGAGGAGATGGAA ACAGCAACTGAGCTCTTTCTGGTGATGGAATTGGTCAAAGGTGGAGATCTCTTTGATGCAATTACTTCG TCGACCAAGTACACTGAGAGAGATGGCAGTGCCATGGTGTACAACTTAGCCAATGCCCTCAGGTATCTC CATGGCCTCAGCATCGTGCACAGAGACATCAAACCAGAGAATCTCTTGGTGTGTGAATATCCTGATGGA ACCAAGTCTTTGAAACTGGGAGACTTTGGGCTTGCGACTGTGGTAGAAGGCCCTTTATACACAGTCTGT GGCACACCCACTTATGTGGCTCCAGAAATCATTGCTGAAACTGGCTATGGCCTGAAGGTGGACATTTGG GCAGCTGGTGTGATCACATACATACTTCTCTGTGGATTCCCACCATTCCGAAGTGAGAACAATCTCCAG GAAGATCTCTTCGACCAGATCTTGGCTGGGAAGCTGGAGTTTCCGGCCCCCTACTGGGATAACATCACG GACTCTGCCAAGGAATTAATCAGTCAAATGCTTCAGGTAAATGTTGAAGCTCGGTGTACCGCGGGACAA ATCCTGAGTCACCCCTGGGTGTCAGATGATGCCTCCCAGGAGAATAACATGCAAGCTGAGGTGACAGGT AAACTAAAACAGCACTTTAATAATGCGCTCCCCAAACAGAACAGCACTACCACCGGGGTCTCCGTCATC ATGAACACGGCTCTAGATAAGGAGGGGCAGATTTTCTGCAGCAAGCACTGTCAAGACAGCGGCAGGCCT GGGATGGAGCCCATCTCTCCAGTTCCTCCCTCAGTGGAGGAGATCCCTGTGCCTGGGGAAGCAGTCCCG GCCCCCACCCCTCCGGAATCTCCCACCCCCCACCCTCCTCCCGCTGCCCCGGGTGGTGAGCGGGCAGGA ACCTGGCGCCGCCACCGAGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001040260 |
| Insert Size | 2301 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001040260.3 |
| RefSeq Size | 4274 bp |
| RefSeq ORF | 2301 bp |
| Locus ID | 166614 |
| UniProt ID | Q8N568 |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 83.6 kDa |
| Gene Summary | This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. Mouse studies show that the DCX gene, another family member, and this gene share function in the establishment of hippocampal organization and that their absence results in a severe epileptic phenotype and lethality, as described in human patients with lissencephaly. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Sep 2010] Transcript Variant: This variant (1) is known as exon-5-lacking transcript (PMIDs:15611072 and 18075264). It lacks an in-frame coding exon in the 5' region, as compared to variant 2. The resulting isoform (a) lacks an internal segment, as compared to isoform b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219931 | DCLK2 (Myc-DDK-tagged)-Human doublecortin-like kinase 2 (DCLK2), transcript variant 1 |
CNY 5,608.00 |
|
| RC219931L1 | Lenti-ORF clone of DCLK2 (Myc-DDK-tagged)-Human doublecortin-like kinase 2 (DCLK2), transcript variant 1 |
CNY 8,008.00 |
|
| RC219931L2 | Lenti-ORF clone of DCLK2 (mGFP-tagged)-Human doublecortin-like kinase 2 (DCLK2), transcript variant 1 |
CNY 9,980.00 |
|
| RC219931L3 | Lenti-ORF clone of DCLK2 (Myc-DDK-tagged)-Human doublecortin-like kinase 2 (DCLK2), transcript variant 1 |
CNY 9,980.00 |
|
| RC219931L4 | Lenti-ORF clone of DCLK2 (mGFP-tagged)-Human doublecortin-like kinase 2 (DCLK2), transcript variant 1 |
CNY 9,980.00 |
|
| RG219931 | DCLK2 (tGFP-tagged) - Human doublecortin-like kinase 2 (DCLK2), transcript variant 1 |
CNY 8,930.00 |
|
| SC323532 | DCLK2 (untagged)-Kinase deficient mutant (K422M) of Human doublecortin-like kinase 2 (DCLK2), transcript variant 1 |
CNY 5,616.00 |
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