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PPHLN1 (NM_016488) Human Untagged Clone
CAT＃: SC310364
PPHLN1 (untagged)-Human periphilin 1 (PPHLN1), transcript variant 1
CNY 7,320.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CR; HSPC206; HSPC232 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC310364 representing NM_016488.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTGGTCTGAGGGACGATATGAATATGAAAGAATTCCGAGAGAACGAGCACCTCCTCGAAGTCATCCC AGTGATGGCTACAATAGACTAGTTAATATTGTGCCAAAGAAACCACCACTGCTAGACAGACCTGGTGAA GGAAGCTACAATAGATATTACAGTCATGTTGATTACCGAGACTATGACGAGGGCCGCAGTTTTTCTCAT GATCGAAGAAGTGGTCCACCTCACAGAGGAGATGAATCTGGTTATAGATGGACAAGAGACGATCATTCT GCAAGCAGGCAACCTGAATACAGGGACATGAGAGATGGCTTTAGAAGAAAAAGTTTCTACTCTTCCCAT TATGCGAGAGAGCGGTCTCCTTATAAAAGGGACAATACTTTTTTCAGAGAATCACCTGTTGGCCGAAAG GATTCTCCACACAGCAGATCTGGTTCCAGTGTCAGTAGCAGAAGCTACTCTCCAGAAAGGAGCAAATCA TACTCTTTCCATCAGTCTCAACATAGAAAGTCCGTGCGTCCTGGTGCCTCCTACAAACGGCAGAATGAA GGAAATCCTGAAAGAGATAAAGAGAGGCCTGTCCAGTCTTTGAAAACATCAAGAGATACTTCACCCTCA AGTGGTTCAGCAGTTTCTTCATCAAAGGTGTTAGACAAACCCAGTAGGCTAACTGAAAAGGAACTTGCT GAGGCTGCAAGCAAGTGGGCTGCTGAAAAGCTAGAGAAATCAGATGAAAGTAACTTGCCTGAAATTTCT GAGTATGAGGCGGGATCCACAGCACCATTGTTTACTGACCAGCCAGAGGAACCTGAGTCAAACACAACA CATGGGATAGAATTATTTGAAGATAGTCAGCTAACCACTCGCTCTAAAGCAATAGCATCAAAAACCAAA GAGATTGAACAGGTTTACCGACAAGACTGTGAAACTTTCGGGATGGTGGTGAAAATGCTGATTGAAAAA GATCCTTCATTAGAAAAGTCTATACAGTTTGCATTGAGGCAGAATTTACATGAAATAGAGTCTGCAGGA CAGACTTGGCAGCAGGTACCCCCTGTGAGGAACACTGAAATGGATCACGACGGAACCCCCGAAAACGAA GGCGAAGAGACTGCGCAGTCAGCCCCGCAGCCCCCGCAGGCCCCGCAGCCCCTGCAGCCCCGGAAGAAA CGGGTTCGTCGGACGACACAGCTTCGTCGGACGACAGGAGCCCCAGACATTACCTGGGGGATGCTGAAG AAGACAACTCAGGAGGCTGAGAGGATCCTGCTCCGAACACAGACACCATTCACTCCAGAAAATTTGTTC CTTGCTATGCTCTCTGTTGTACATTGCAACTCACGCAAGGATGTAAAGCCAGAAAACAAGCAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_016488 |
| Insert Size | 1377 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016488.6 |
| RefSeq Size | 3484 bp |
| RefSeq ORF | 1377 bp |
| Locus ID | 51535 |
| UniProt ID | Q8NEY8 |
| MW | 52.7 kDa |
| Gene Summary | The protein encoded by this gene is one of the several proteins that become sequentially incorporated into the cornified cell envelope during the terminal differentiation of keratinocyte at the outer layers of epidermis. This protein interacts with periplakin, which is known as a precursor of the cornified cell envelope. The cellular localization pattern and insolubility of this protein suggest that it may play a role in epithelial differentiation and contribute to epidermal integrity and barrier formation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215736 | PPHLN1 (Myc-DDK-tagged)-Human periphilin 1 (PPHLN1), transcript variant 1 |
CNY 3,656.00 |
|
| RC215736L1 | Lenti ORF clone of Human periphilin 1 (PPHLN1), transcript variant 1, Myc-DDK-tagged |
CNY 5,990.00 |
|
| RC215736L2 | Lenti ORF clone of Human periphilin 1 (PPHLN1), transcript variant 1, mGFP tagged |
CNY 5,990.00 |
|
| RC215736L3 | Lenti ORF clone of Human periphilin 1 (PPHLN1), transcript variant 1, Myc-DDK-tagged |
CNY 5,990.00 |
|
| RC215736L4 | Lenti ORF clone of Human periphilin 1 (PPHLN1), transcript variant 1, mGFP tagged |
CNY 5,990.00 |
|
| RG215736 | PPHLN1 (tGFP-tagged) - Human periphilin 1 (PPHLN1), transcript variant 1 |
CNY 4,470.00 |
