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MDMX (MDM4) (NM_002393) Human Untagged Clone
CAT＃: SC310334
MDM4 (untagged)-Human Mdm4 p53 binding protein homolog (mouse) (MDM4), transcript variant 1
CNY 5,488.00
| Cited in 2 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BMFS6; HDMX; MDMX; MRP1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_002393 edited
TGGAGCTGCCGTAAGTTTTACCAACAGACTGCAGTTTCTTCACTACCAAAATGACATCAT TTTCCACCTCTGCTCAGTGTTCAACATCTGACAGTGCTTGCAGGATCTCTCCTGGACAAA TCAATCAGGTACGACCAAAACTGCCGCTTTTGAAGATTTTGCATGCAGCAGGTGCGCAAG GTGAAATGTTCACTGTTAAAGAGGTCATGCACTATTTAGGTCAGTACATAATGGTGAAGC AACTTTATGATCAGCAGGAGCAGCATATGGTATATTGTGGTGGAGATCTTTTGGGAGAAC TACTGGGACGTCAGAGCTTCTCCGTGAAAGACCCAAGCCCTCTCTATGATATGCTAAGAA AGAATCTTGTCACTTTAGCCACTGCTACTACAGATGCTGCTCAGACTCTCGCTCTCGCAC AGGATCACAGTATGGATATTCCAAGTCAAGACCAACTGAAGCAAAGTGCAGAGGAAAGTT CCACTTCCAGAAAAAGAACTACAGAAGACGATATCCCCACACTGCCTACCTCAGAGCATA AATGCATACATTCTAGAGAAGATGAAGACTTAATTGAAAATTTAGCCCAAGATGAAACAT CTAGGCTGGACCTTGGATTTGAGGAGTGGGATGTAGCTGGCCTGCCTTGGTGGTTTTTAG GAAACTTGAGAAGCAACTATACACCTAGAAGTAATGGCTCAACTGATTTACAGACAAATC AGGATGTGGGTACTGCCATTGTTTCAGATACTACAGATGACTTGTGGTTTTTGAATGAGT CAGTATCAGAGCAGTTAGGTGTTGGAATAAAAGTTGAAGCTGCTGATACTGAACAAACAA GTGAAGAAGTAGGGAAAGTAAGTGACAAAAAGGTGATTGAAGTGGGAAAAAATGATGACC TGGAGGACTCTAAGTCCTTAAGTGATGATACCGATGTAGAGGTTACCTCTGAGGATGAGT GGCAGTGTACTGAATGCAAGAAATTTAACTCTCCAAGCAAGAGGTACTGTTTTCGTTGTT GGGCCTTGAGGAAGGATTGGTATTCAGATTGTTCAAAGTTAACCCATTCTCTCTCCACGT CTGATATCACTGCCATACCTGAAAAGGAAAATGAAGGAAATGATGTCCCTGATTGTCGAA GAACCATTTCGGCTCCTGTCGTTAGACCTAAAGATGCGTATATAAAGAAAGAAAACTCCA AACTTTTTGATCCCTGCAACTCAGTGGAATTCTTGGATTTGGCTCACAGTTCTGAAAGCC AAGAGACCATCTCAAGCATGGGAGAACAGTTAGATAACCTTTCTGAACAGAGAACAGATA CAGAAAACATGGAGGATTGCCAGAATCTCTTGAAGCCATGTAGCTTATGTGAGAAAAGAC CACGAGACGGGAACATTATTCATGGAAGGACGGGCCATCTTGTCACTTGTTTTCACTGTG CCAGAAGACTAAAGAAGGCTGGGGCTTCATGCCCTATTTGCAAGAAAGAGATTCAGCTGG TTATTAAGGTTTTTATAGCATAATGGTAGTACGAACATAAAAATGCATTTATTCAGTTCA CTTACCACATTATTTGAAAATCAATCCTTTATTTAATTTTATTTCCAACCTGTCAGAGAA TGTTCTTAGGCATCAAAATCCAAGGTAGCTG >OriGene 5' read for NM_002393 unedited
GTCAAGGTCAGAATTGTATACGACTCATATAGGCGGCCGCCAGTGTGATGGAATCTGCAG AATTCGCCCTTTGGAGCTGCCGTAAGTTTTACCAACAGACTGCAGTTTCTTCACTACCAA AATGACATCATTTTCCACCTCTGCTCAGTGTTCAACATCTGACAGTGCTTGCAGGATCTC TCCTGGACAAATCAATCAGGTACGACCAAAACTGCCGCTTTTGAAGATTTTGCATGCAGC AGGTGCGCAAGGTGAAATGTTCACTGTTAAAGAGGTCATGCACTATTTAGGTCAGTACAT AATGGTGAAGCAACTTTATGATCAGCAGGAGCAGCATATGGTATATTGTGGTGGAGATCT TTTGGGAGAACTACTGGGACGTCAGAGCTTCTCCGTGAAAGACCCAAGCCCTCTCTATGA TATGCTAAGAAAGAATCTTGTCACTTTAGCCACTGCTACTACAGATGCTGCTCAGACTCT CGCTCTCGCACAGGATCACAGTATGGATATTCCAAGTCAAGACCAACTGAAGCAAAGTGC AGAGGAAAGTTCCACTTCCAGAAAAAGAACTACAGAAGACGATATCCCCACACTGCCTAC CTCAGAGCATAAATGCATACATTCTAGAGAAGATGAAGACTTAATTGAAAATTTAGCCCA AGATGAAACATCTAGGCTGGACCTTNGGATTGAGGAGTGGGATGTAGCTGGCCTGCCTTG GTGGTTTTTAGGAAACTTGAGAAGCACTATACACTAGGAAGTATGGCTCAACTGATTTAC AGACAAATCANGATGTGGGGTACTGGCCATTGTTTCAGATACTACAGATGACTTGTGGGT TTTTGAATGAATCAGTATCAGAAGCGTTTAGGTGTTTGGATAA >OriGene 3' genomic read for NM_002393 unedited
CTTTGGNNGATGGCACTTNCCAGGNCCAGNAAAGCACTGGGGNAGGGTCACAGGATGCCA CCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCGAATTCAGATCTGGTACCGAG CTCGGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGCCCTTCAGCTACCTTGGAT TTTGATGCCTAAGAACATTCTCTGACAGGTTGGAAATAAAATTAAATAAAGGATTGATTT TCAAATAATGTGGTAAGTGAACTGAATAAATGCATTTTTATGTTCGTACTACCATTATGC TATAAAAACCTTAATAACCAGCTGAATCTCTTTCTTGCAAATAGGGCATGAAGCCCCAGC CTTCTTTAGTCTTCTGGCACAGTGAAAACAAGTGACAAGATGGCCCGTCCTTCCATGAAT AATGTTCCCGTCTCGTGGTCTTTTCTCACATAAGCTACATGGCTTCAAGAGATTCTGGCA ATCCTCCATGTTTTCTGTATCTGTTCTCTGTTCAGAAAGGTTATCTAACTGTTCTCCCAT GCTTGAGATGGTCTCTTGGCTTTCAGAACTGTGAGCCAAATCCAAGAATTCCACTGAGTT GCAGGGATCAAAAAGTTTGGAGTTTTCTTTCTTTATATACGCATCTTTAGGTCTAACGAC AGGAGCCGAAATGGTTCTTCGACAATCAGGGACATCATTTCCTTCATTTTCCTTTTCAGG TATGGCAGTGATATCAGACGTGGAGAGAGAATGGGTTAACTTTGAACAATCTGAATACCA ATCCTTCCTCAGGCCCCAACACGAAAACAGTACCTCTTGCTTGGAGAGTAAAATTTCTGC TTCAGTACACTGCCACTCATCCTAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_002393 |
| Insert Size | 1700 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | There are 1 nucleotide differences between the OriGene clone and the NCBI reference ORF. OriGene considers these to be polymorphisms and to reflect the natural differences between individuals. These result in the substitution of 1 amino acid. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002393.2, NP_002384.2 |
| RefSeq Size | 2554 bp |
| RefSeq ORF | 1473 bp |
| Locus ID | 4194 |
| UniProt ID | O15151 |
| Domains | SWIB |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | p53 signaling pathway |
| Gene Summary | This gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Feb 2011] Transcript Variant: This variant (1, also known as MDM4-FL or HDMX) represents the predominant transcript, and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Searching for dual inhibitors of the MDM2-p53 and MDMX-p53 protein-protein interaction by a scaffold-hopping approach
,Zaytsev, A;Dodd, B;Magnani, M;Ghiron, C;Golding, BT;Griffin, RJ;Liu, J;Lu, X;Micco, I;Newell, DR;Padova, A;Robertson, G;Lunec, J;Hardcastle, IR;,
Chem Biol Drug Des
,PubMed ID 25388787
[MDM4]
|
|
EEF1A2 inactivates p53 via PI3K/AKT/mTOR-dependent stabilization of MDM4 in hepatocellular carcinoma
,Pellegrino, R;Calvisi, DF;Neumann, O;Kolluru, V;Wesely, J;Chen, X;Wang, C;Wuestefeld, T;Ladu, S;Elgohary, N;Bermejo, JL;Radlwimmer, B;Zörnig, M;Zender, L;Dombrowski, F;Evert, M;Schirmacher, P;Longerich, T;,
Hepatology
,PubMed ID 24285179
[MDM4]
|
Documents
| Product Manuals |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209620 | MDM4 (Myc-DDK-tagged)-Human Mdm4 p53 binding protein homolog (mouse) (MDM4), transcript variant 1 |
CNY 5,488.00 |
|
| RC209620L1 | Lenti ORF clone of Human Mdm4 p53 binding protein homolog (mouse) (MDM4), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC209620L2 | Lenti ORF clone of Human Mdm4 p53 binding protein homolog (mouse) (MDM4), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC209620L3 | Lenti ORF clone of Human Mdm4 p53 binding protein homolog (mouse) (MDM4), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC209620L4 | Lenti ORF clone of Human Mdm4 p53 binding protein homolog (mouse) (MDM4), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| RG209620 | MDM4 (tGFP-tagged) - Human Mdm4 p53 binding protein homolog (mouse) (MDM4), transcript variant 1 |
CNY 7,088.00 |
|
| SC322031 | MDM4 (untagged)-Human Mdm4 p53 binding protein homolog (mouse) (MDM4), transcript variant 1 |
CNY 5,488.00 |
