PPP2R5D (NM_180977) Human Untagged Clone

CAT#: SC310326

PPP2R5D (untagged)-Human protein phosphatase 2, regulatory subunit B', delta (PPP2R5D), transcript variant 3



  "NM_180977" in other vectors (4)

CNY 7,980.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
PPP2R5D mouse monoclonal antibody, clone OTI5E7 (formerly 5E7)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms B56D; B56delta; MRD35
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC310326 representing NM_180977.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCCCTATAAACTGAAAAAGGAGAAGGAGCTGTTTATCCAGAAGCTACGCCAGTGCTGTGTCCTCTTT
GACTTCGTGTCAGACCCACTCAGTGACCTCAAATTCAAGGAGGTGAAGCGGGCAGGACTCAACGAGATG
GTGGAGTACATCACCCATAGCCGTGATGTTGTCACTGAGGCCATTTACCCTGAGGCTGTCACCATGTTT
TCAGTGAACCTCTTCCGGACGCTGCCACCTTCATCGAATCCCACAGGGGCTGAGTTTGACCCAGAGGAA
GATGAGCCCACCCTGGAAGCTGCTTGGCCACATCTCCAGCTCGTGTATGAGTTCTTCTTACGTTTCCTT
GAGTCTCCTGATTTCCAGCCAAACATAGCCAAGAAGTACATCGACCAGAAGTTTGTACTTGCTCTCCTA
GACCTATTTGACAGTGAGGATCCTCGAGAGCGGGACTTCCTCAAGACCATTTTGCATCGCATCTATGGC
AAGTTTTTGGGGCTCCGGGCTTATATCCGTAGGCAGATCAACCACATCTTCTACAGGTTCATCTACGAG
ACGGAGCATCACAACGGGATTGCTGAGCTCCTGGAGATCCTGGGCAGCATCATCAATGGCTTTGCCCTG
CCCCTTAAAGAAGAGCACAAGATGTTCCTCATCCGTGTCCTACTTCCCCTTCACAAGGTCAAGTCCCTG
AGTGTCTACCACCCTCAGCTGGCATACTGTGTGGTACAATTCCTGGAGAAGGAGAGCAGTCTGACTGAG
CCGGTAATTGTGGGACTTCTCAAGTTTTGGCCCAAGACCCACAGCCCCAAGGAGGTGATGTTCTTGAAT
GAGCTGGAGGAGATTCTGGACGTCATTGAACCTTCTGAGTTCAGCAAAGTGATGGAACCCCTCTTCCGC
CAGCTGGCCAAGTGTGTCTCTAGCCCCCATTTCCAGGTGGCAGAGCGTGCTCTCTATTACTGGAACAAT
GAGTACATCATGAGCCTGATAAGTGACAATGCTGCCCGAGTCCTCCCCATCATGTTCCCTGCACTCTAC
AGGAACTCCAAGAGCCACTGGAACAAGACAATCCATGGACTGATCTATAATGCCCTGAAGTTGTTTATG
GAAATGAATCAGAAGCTGTTTGATGACTGCACACAACAATACAAGGCAGAGAAGCAGAAGGGCCGGTTC
CGAATGAAGGAAAGGGAAGAGATGTGGCAAAAAATCGAGGAGCTGGCCCGGCTTAATCCCCAGTATCCC
ATGTTCCGAGCCCCTCCACCACTGCCCCCTGTGTACTCGATGGAGACAGAGACCCCCACAGCTGAGGAC
ATCCAGCTTCTGAAGAGGACTGTGGAGACTGAGGCTGTTCAGATGCTAAAAGACATCAAGAAGGAGAAA
GTGCTGCTGCGGAGGAAGTCGGAGCTGCCCCAGGACGTGTACACCATCAAGGCACTGGAGGCGCACAAG
CGGGCGGAAGAGTTCCTAACTGCCAGCCAGGAGGCTCTCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_180977
Insert Size 1491 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_180977.2
RefSeq Size 2747 bp
RefSeq ORF 1491 bp
Locus ID 5528
UniProt ID Q14738
Protein Families Phosphatase
Protein Pathways Oocyte meiosis, Wnt signaling pathway
MW 58.5 kDa
Gene Summary The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a delta isoform of the regulatory subunit B56 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (3) lacks an in-frame segment of the coding region, compared to variant 1. It encodes a shorter isoform (3), that is missing an internal segment compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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